GB/T 40744-2021Determination of solanesol in potato stems， leaves and their processed products—High performance liquid chromatography-mass spectrometry (English PDF)

Foreword

This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for Standardization Work Part 1: Structure and Drafting Rules of Standardization Documents" Drafting.

1 Scope

This document specifies the principle and test for the determination of total solanesol content in potato stems and leaves and its processed products by high performance liquid chromatography-mass spectrometry. Agents and materials, instruments and equipment, testing procedures, expression of analysis results, precision, etc.

This document is applicable to the determination of the total solanesol content in potato stems and leaves and their processed products.

2 Normative references

The content of the following documents constitutes an indispensable clause of this document through normative references in the text. Among them, dated quotations Only the version corresponding to the date is applicable to this document; for undated reference documents, the latest version (including all amendments) is applicable to this document.

GB/T 6682 Analytical laboratory water specifications and test methods

GB/T 8855 Sampling method for fresh fruits and vegetables

3 Terms and definitions

There are no terms and definitions that need to be defined in this document.

4 Principle

The free solanesol (including the ester state) in potato leaves is extracted with n-hexane, and the total solanesol is saponified with sodium hydroxide to release the bound state. Solanesol, then remove impurities with water, centrifuge, take the upper solution diluted with mobile phase to volume, filter through a membrane and use high performance liquid chromatography- Determination by mass spectrometer, quantitative calculation by external standard method.

5 Reagents and materials

Unless otherwise specified, the reagents used are all analytically pure, and the water should meet the requirements of first-grade water in GB/T 6682.

5.1 n-hexane (C6H14). analytically pure.

5.2 Methanol (CH4O). chromatographically pure.

5.3 Isopropanol (C3H8O). chromatographically pure.

5.4 Sodium hydroxide (NaOH). analytically pure.

5.5 Absolute ethanol (C2H6O) is analytically pure.

5.6 Solanesol standard product (C45H74O, CAS number. 13190-97-1). purity >=98%.

5.7 NaOH ethanol solution (0.02mol/L). Weigh 0.4g sodium hydroxide (5.4), dissolve it in absolute ethanol (5.5), and dilute to 500mL, stored in a polyethylene bottle, screw the cap tightly.

5.8 Mobile phase. methanol (5.2) : isopropanol (5.3) = 50:50 (volume ratio), filtered through an organic phase filter membrane (5.11) before use, and ultrasonically degassed.

5.9 Solanesol standard stock solution (500µg/mL). Weigh 50mg solanesol standard product (5.6), accurate to 0.0001g, and place it in 100mL […]