GB/T 45538-2025Determination of plant extracts antioxidant activity — Thin layer chromatography method (English PDF)

Foreword

This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for standardization work Part 1: Structure and drafting rules for standardization documents" Drafting.

Please note that some of the contents of this document may involve patents. The issuing organization of this document does not assume the responsibility for identifying patents.

This document is proposed and coordinated by the China National Institute of Standardization.

This document was drafted by: Jiangnan University, Shenzhen Institute of Standards and Technology, China National Institute of Standardization, Shenzhen Analysis and Testing Association, Shanxi Agricultural University, Beijing Tongrentang Health Pharmaceutical Co., Ltd., Beijing Academy of Science and Technology Analysis and Testing Institute (Beijing Institute of Chemical Analysis and Testing Center), Laiyang Inspection and Testing Center, Infinitus (China) Co., Ltd., Yunnan Yunke Special Plant Extraction Laboratory Co., Ltd.

Co., Ltd., Hebei Meiye Siwei Biotechnology Co., Ltd., Guangdong Yifang Pharmaceutical Co., Ltd., Nanjing Zhongke Pharmaceutical Co., Ltd., China Metrology Science Research Institute Institute, Liheweidao (Qingdao) Food Industry Co., Ltd., Food and Drug Environmental Inspection Institute (Shandong) Group Co., Ltd., Ingel Testing and Certification Certificate (Shandong) Co., Ltd.

The main drafters of this document are: Chen Yisheng, Zha Shenghua, Zhan Songkun, Zhang Hong, Wang Ke, Ouyang Jing, Xi Xingjun, Bian Ruifang, Shi Xudong, Xu Xueming, Wang Wei, Tao Hong, Lu Zhi, Wang Feifei, Liu Rui, Luo Wenhui, Lv Weisheng, Zhou Yajie, Feng Peng, Wang Shanshan, Zhang Yongchang, Liu Mingsheng, Xu Mingyu, Sun Xili, Li Yanhong, Xu Hong, Meng Xia, Wang Yanwei, Luo Bing.

Determination of antioxidant activity of plant extracts by thin layer chromatography

1 Scope

This document describes a method for determining the antioxidant activity of plant extracts by thin layer chromatography.

This document is applicable to the determination of antioxidant activity of extracts of chamomile, plantain, forsythia, bamboo leaves or sophora japonica, and the antioxidant activity of other plant extracts.

Oxidative activity assay was used as a reference.

2 Normative references

GB/T 6682

3 Terms and definitions

The following terms and definitions apply to this document.

4 Principle

Thin layer chromatography separation principle. Since the chromatographic stationary phase has different antioxidant activities in plant extracts (such as phenols, flavonoids The adsorption capacity of the mobile phase is different. When the mobile phase flows through the stationary phase, adsorption, desorption, re-adsorption and re-desorption will occur continuously.

This allows compounds with different antioxidant activities to be separated from each other.

2,2-Biphenyl-1-picrylhydrazyl (DPPH) impregnation color development principle. DPPH has a dark purple appearance and is evenly applied to a thin layer of color by impregnation.

The free radical scavenger on the stationary phase reacts with DPPH to significantly lighten its color, forming a light yellow on a dark purple background. Color spots.

The compounds with different antioxidant activities in plant extracts were separated by thin layer chromatography and DPPH immersion was used to analyze the antioxidant activity of the compounds.

Color reaction (the result of the color reaction is quantitatively detected by a thin layer chromatography scanner). The peak area of rutin is used as the reference peak area.

The total peak area of the plant extract was taken as the sample peak area, and the comprehensive peak area of the plant extract was expressed by the ratio of the sample peak area to the control peak area (Ai). Antioxidant activity.

5 Reagents and Materials

Unless otherwise stated, all reagents used in the analysis were of analytical grade.