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GB/T 23599-2025Pure culture of straw mushroom (English PDF)

草菇菌种

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Issued by

SAMR; SAC

Level / Type

National · Recommended

Issue date

June 30, 2025

Implementation date

January 1, 2026

Scope

GB/T 23599-2025 is the English-translated version of 草菇菌种.

GB/T 23599-2025 is the Chinese national standard covering the spawn from which Volvariella volvacea is grown — the parent, original and cultivated stock and now the liquid stock as well, the sensory requirements for each and the container specifications, the tests for angular sectoring and for the high-temperature inhibition line that betray a culture already damaged, the inspection and judgement rules, and the marking, packaging, transport and storage. The 2025 edition drops the microbiological, electrophoresis and ITS sequence requirements and adds liquid stock. It replaces GB/T 23599-2009, under the All China Federation of Supply and Marketing Cooperatives. In force from 1 January 2026. Issued on 30 June 2025, it has been in force since 1 January 2026, replacing GB/T 23599-2009.

Document preview — GB/T 23599-2025

National Standard of the People's Republic of China

ICS
65.020.99
Classification
B 39
Replacing
GB/T 23599-2009

Issued by: State Administration for Market Regulation; Standardization Administration of the PRC

Contents

  • Foreword
  • 1 Scope
  • 2 Normative references
  • 3 Terms and Definitions
  • 3.1 [Source. GB/T 12728-2006, 2.3.21, modified]
  • 3.2 Angular sectoring
  • 3.3 high-temperature line
  • 3.4 aerial hyphae

Foreword

This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for standardization work Part 1: Structure and drafting rules for standardization documents" Drafting.

This document replaces GB/T 23599-2009 “Volagus Species”. Compared with GB/T 23599-2009, except for structural adjustments and editorial changes, In addition to the dynamic, the main technical changes are as follows.

a) Added the terms and definitions of aerial hyphae, chlamydospores, primordia, biological efficiency and liquid culture (see 3.4 to 3.8);

b) The sensory requirements for mother stocks, solid stock and cultivars have been changed (see 4.1.2, 4.2.1.2 and 4.3.2, 4.1.2 and 4.2.2 of the 2009 edition). and 4.3.2);

c) The microbiological requirements for parent stocks, original stocks and cultivars have been deleted (see 4.1.3, 4.2.3 and 4.3.3 of the 2009 edition);

d) Deleted the electrophoresis pattern characteristics of mycelial soluble proteins, electrophoresis method and PDY medium formula in the electrophoresis method (see 2009 4.1.6, 5.4 and Appendix C of the IEEE International Standards and Regulations (2003);

e) Deleted ITS (internal transcribed spacer) sequence characteristics, analysis methods and analysis methods (see 4.1.7, 5.5 and Appendix of the 2009 edition) D);

f) The container specifications for solid stock seeds and cultivated seeds have been changed (see 4.2.1.1, 4.3.1, 4.2.1, 4.3.1 of the 2009 edition);

g) Added liquid stock (see 4.2.2);

h) The test method has been changed (see Chapter 5, Chapter 5 of the 2009 edition);

i) The judgment rules have been changed (see 6.2, 6.2 of the 2009 edition);

j) Changed the transport requirements (see 7.3, 7.3 of the 2009 edition);

k) The storage requirements have been changed (see 7.4, 7.4 of the 2009 edition).

Please note that some of the contents of this document may involve patents. The issuing organization of this document does not assume the responsibility for identifying patents.

This document is proposed and coordinated by the China Federation of Supply and Marketing Cooperatives.

This document was drafted by: Kunming Edible Fungi Research Institute of China National Federation of Supply and Marketing Cooperatives, Institute of Microbiology of Guangdong Academy of Sciences, Jiangsu An Hui Biotechnology Co., Ltd., Yunnan Edible Fungi Industry Development Research Institute, Yunnan Yunjun Technology (Group) Co., Ltd., Yunnan Supply and Marketing Cooperative Social Union, China National Federation of Supply and Marketing Cooperatives Nanjing Institute of Comprehensive Utilization of Wild Plants.

The main drafters of this document are: Hua Rong, Sun Dafeng, Wu Qingping, Liu Shaoxiong, Li Xuesong, Hu Huiping, Chen Junhua, Shang Lu'e, Wu Weijie, Yang Xiaobing, Dong Jiao, Zhao Chunyan, Yue Wansong, Liu Yinshan, He Jun, Deng Yayuan, Liu Yuanchao, Cai Manjun, Zhang Xiaohua, Huang Longhua, Gao Zhanghui, Lin Qunying, Wu Liangliang and Wu Xiaoxian.

The previous versions of this document and the documents it replaces are as follows.

— First published in 2009 as GB/T 23599-2009;

— This is the first revision. Straw mushroom strains

1 Scope

This document specifies the quality requirements, inspection rules, and marking, labeling, packaging, transportation and storage requirements of Volvariella volvacea. Species inspection method.

This document applies to the production, circulation and use of Volvariella volvacea strains.

2 Normative references

GB/T 191

GB/T 12728

NY/T 528-2010

NY/T 1284

3 Terms and Definitions

The terms and definitions defined in GB/T 12728 and the following apply to this document.

3.1 [Source. GB/T 12728-2006, 2.3.21, modified]

The phenomenon that colonies with different genetic genes inhibit each other to produce no-growth zones or form linear edges of different forms.

3.2 Angular sectoring

The phenomenon that the mycelium becomes thinner, grows slowly, and has abnormal angular surface features due to local mutation of the mycelium or infection by a virus.

[Source. GB/T 12728-2006, 2.5.17]

3.3 high-temperature line

High temperature suppression line During the cultivation process, edible fungi are adversely affected by high temperature and insufficient ventilation, and the culture appears to be ring-shaped yellow, dark, or the mycelium becomes thinner. Weak phenomenon.

[Source. GB/T 12728-2006, 2.5.18, modified]

3.4 aerial hyphae

Mycelium growing in the surface space of the culture medium.

[Source. GB/T 12728-2006, 2.2.7]

......
This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 15 pages — is available in the English PDF.

Referenced standards

Editions of GB/T 23599

EditionTitleRevisionStatus
GB/T 23599-2025Pure culture of straw mushroomcurrent editionCurrent
GB/T 23599-2009Pure culture of straw mushroomprevious editionIn force

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