GB/T 28630.4-2012Diagnostic protocols for white spot disease - Part 4: Histopathology method (English PDF)
白斑综合征(WSD)诊断规程 第4部分：组织病理学诊断法
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Issued by
SAMR; SAC
Level / Type
National · Recommended
Issue date
July 31, 2012
Implementation date
November 1, 2012
Scope
GB/T 28630.4-2012 is the English-translated version of 白斑综合征(WSD)诊断规程 第4部分：组织病理学诊断法.
GB/T 28630.4-2012 is the Chinese standard "Diagnostic protocols for white spot disease - Part 4: Histopathology method". Its scope clause reads: This section GB/T 28630 specifies the necessary reagents and materials, equipment, procedures spot syndrome histopathological diagnosis method And the results of determination.
This section applies to the onset of shrimp or other sensitive host confirmed and suspected infected hosts diagnosis. This section does not apply to latent infection or noninfectious virus carrying the specimens tested for the virus.
Principle 2 Pathogens and pathological features of White Spot Syndrome 2.1 White Spot Syndrome (whitespotsyndrome), also known as vitiligo (whitespotdisease), white spot syndrome, shrimp fulminant epidemic disease.
Spot Syndrome has acute onset and high mortality characteristics, symptoms onset stop feeding shrimp, unresponsive, or lying poolside or Floating in the water, some dying shrimp appear white patches on the carapace, sometimes accompanied by abdominal segment slightly red shrimp.
White Spot Syndrome pathogen is white spot virus (whitespotsyndromevirus, WSSV) also known as white spot syndrome virus (white spotvirus, WSV), which is a double-stranded DNA (dsDNA) viruses that can cause the majority of farmed shrimp has a fatal infection. virus Oblong particles having a capsule and a unique tail-like, diameter 120nm ~ 150nm, length 270nm ~ 290nm, its genome size About 300kb.
Virus replication in the nucleus, do not form a closed body (occlusionbody).
Moribund shrimp showed lesions in systemic ectodermal and mesodermal origin of the tissue, the virus infects the epithelial tissue includes a lower crustaceans (including Including surface and under the skin appendages of crustaceans, gastric epithelial cells and intestinal epithelium), hematopoietic tissue, gill, connective tissue, antennal gland, blood and lymphoid organs Wait.
Inclusion (inclusion) infection early in the nucleus was formed eosinophilic, with the rapid replication of the virus, causing the nuclear chromatin margination, Mast cell nucleus, basophilic inclusion bodies are getting stronger. Its clauses include reagents and materials.
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Document preview — GB/T 28630.4-2012
National Standard of the People's Republic of China
- ICS
- 65.020.30
- Classification
- B 41
Issued by: State Administration for Market Regulation; Standardization Administration of the PRC
Contents
- 1 Scope
- 2.2 Technical Principle
- 3 Reagents and materials
- 3.6 filtered seawater
1 Scope
This section GB/T 28630 specifies the necessary reagents and materials, equipment, procedures spot syndrome histopathological diagnosis method
And the results of determination.
This section applies to the onset of shrimp or other sensitive host confirmed and suspected infected hosts diagnosis.
This section does not apply to latent infection or noninfectious virus carrying the specimens tested for the virus.
Principle 2
Pathogens and pathological features of White Spot Syndrome 2.1
White Spot Syndrome (whitespotsyndrome), also known as vitiligo (whitespotdisease), white spot syndrome, shrimp fulminant epidemic disease. Spot Syndrome has acute onset and high mortality characteristics, symptoms onset stop feeding shrimp, unresponsive, or lying poolside or
Floating in the water, some dying shrimp appear white patches on the carapace, sometimes accompanied by abdominal segment slightly red shrimp.
White Spot Syndrome pathogen is white spot virus (whitespotsyndromevirus, WSSV) also known as white spot syndrome virus (white spotvirus, WSV), which is a double-stranded DNA (dsDNA) viruses that can cause the majority of farmed shrimp has a fatal infection. virus
Oblong particles having a capsule and a unique tail-like, diameter 120nm ~ 150nm, length 270nm ~ 290nm, its genome size
About 300kb. Virus replication in the nucleus, do not form a closed body (occlusionbody).
Moribund shrimp showed lesions in systemic ectodermal and mesodermal origin of the tissue, the virus infects the epithelial tissue includes a lower crustaceans (including
Including surface and under the skin appendages of crustaceans, gastric epithelial cells and intestinal epithelium), hematopoietic tissue, gill, connective tissue, antennal gland, blood and lymphoid organs
Wait. Inclusion (inclusion) infection early in the nucleus was formed eosinophilic, with the rapid replication of the virus, causing the nuclear chromatin margination,
Mast cell nucleus, basophilic inclusion bodies are getting stronger.
2.2 Technical Principle
Hematoxylin - eosin (HE) staining is a conventional staining method in histology and pathology. By staining solution --- hematoxylin - eosin
Were organized with the combination of different components of the cell, so that the nucleus, cytoplasm and other structures within the cell to produce a clear contrast between the blue and purple and red
Color, and it can be clearly observed in various pathological changes in the structure of various organizations.
3 Reagents and materials
3.1
Unless otherwise stated in the analysis using only recognized as analytical grade reagents and distilled or deionized water or equivalent purity.
3.2
paraffin wax (melting point 52 °C ~ 54 °C). Slice.
3.3
xylene.
3.4
ethanol.
3.6 filtered seawater
salinity of seawater 20 to 30 in the room for one week, the filter paper and 0.22µm membrane filter.
3.7-Davidson's AFA (Davidson'sAFA) fixative. See Appendix A of the provisions.
......
This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 15 pages — is available in the English PDF.
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Related Standards
GB/T 28630.5-2012 — Diagnostic protocols for white spot disease - Part 5: T-E staining method with fresh tissue
GB/T 28630.3-2012 — Diagnostic protocols for white spot disease - Part 3: In situ hybridization method
GB/T 28630.2-2012 — Diagnostic protocols for white spot disease - Part 2: Nested PCR method
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