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GB/T 28630.5-2012Diagnostic protocols for white spot disease - Part 5: T-E staining method with fresh tissue (English PDF)

白斑综合征(WSD)诊断规程第5部分：新鲜组织的T-E染色法

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Issued by

SAMR; SAC

Level / Type

National · Recommended

Issue date

July 31, 2012

Implementation date

November 1, 2012

Scope

GB/T 28630.5-2012 is the English-translated version of 白斑综合征(WSD)诊断规程第5部分：新鲜组织的T-E染色法.

GB/T 28630.5-2012 is the Chinese standard "Diagnostic protocols for white spot disease - Part 5: T-E staining method with fresh tissue". Its scope clause reads: This section GB/T 28630 regulations required TE Spot Syndrome fresh tissue staining reagents and materials, equipment, operation Steps and results determined.

This section applies to diagnostic laboratories in the field or the sick dying shrimp shrimp larvae, juvenile or adult shrimp for rapid diagnosis, it does not apply Noninfectious specimens carry latent infection or viral diagnosis. Its clauses include reagents and materials; equipment.

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Document preview — GB/T 28630.5-2012

National Standard of the People's Republic of China

ICS: 65.020.30
Classification: B 41

Issued by: State Administration for Market Regulation; Standardization Administration of the PRC

Foreword
1 Scope
2 Normative references
3.2 Technical Principle
4 Reagents and materials

1 Scope

This section GB/T 28630 regulations required TE Spot Syndrome fresh tissue staining reagents and materials, equipment, operation

Steps and results determined.

This section applies to diagnostic laboratories in the field or the sick dying shrimp shrimp larvae, juvenile or adult shrimp for rapid diagnosis, it does not apply

Noninfectious specimens carry latent infection or viral diagnosis.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated edition applies to this article

Member. Where not dated references, the latest edition (including any amendments) applies to this document.

Diagnostic protocols GB/T 28630.4-2012 spot syndrome (WSD) Part 4. Histopathological diagnosis method

Principle 3

Pathogens and pathological features of White Spot Syndrome 3.1

See GB/T 28630.4-2012 2.1.

3.2 Technical Principle

Trypan blue containing a weakly basic group and a strongly acidic group, weakly basic groups which may be combined with a nucleic acid, and strongly acidic groups exist

In so weak acid and trypan blue dye eosin intermixing without precipitation occurs. Thus, a mixture of trypan blue and eosin will be able to make fresh once

Tissue infected with blue nuclei, cytoplasm can be infected red.

4 Reagents and materials

4.1

Unless otherwise stated in the analysis using only recognized as analytical grade reagents and distilled or deionized water or equivalent purity.

4.2 TE Staining Solution

See Appendix A of the provisions.

5 Equipment

5.1

scalpel.

5.2

pointed tweezers.

5.3

medical scissors.

5.4

slide.

5.5

coverslip.

5.6 Optical Microscope

with high magnification (40 ×) and oil immersion objective (100 ×).

......
This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 15 pages — is available in the English PDF.

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