GB/T 28630.2-2012Diagnostic protocols for white spot disease - Part 2: Nested PCR method (English PDF)
白斑综合征(WSD)诊断规程 第2部分：套式PCR检测法
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Issued by
SAMR; SAC
Level / Type
National · Recommended
Issue date
July 31, 2012
Implementation date
November 1, 2012
Scope
GB/T 28630.2-2012 is the English-translated version of 白斑综合征(WSD)诊断规程 第2部分：套式PCR检测法.
GB/T 28630.2-2012 is the Chinese standard "Diagnostic protocols for white spot disease - Part 2: Nested PCR method". Its scope clause reads: This section GB/T 28630 specifies the nested PCR for detection of White Spot Syndrome pathogen required reagents and materials, equipment, Procedure and result judgment.
This section applies to a variety of samples of shrimp, a variety of biological and environmental samples of food organisms, and various other non-biological samples for White Spot Syndrome pathogens with high sensitivity qualitative detection of infected cases, for pathogen screening and diagnosis of diseases.
When used alone in this section do not apply to activity estimation and evaluation of the degree of host infected with the virus or the amount of infection. Its clauses include reagents and materials.
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Document preview — GB/T 28630.2-2012
National Standard of the People's Republic of China
- ICS
- 65.020.30
- Classification
- B 41
Issued by: State Administration for Market Regulation; Standardization Administration of the PRC
Contents
- 1 Scope
- 2 Normative references
- 3.2 Technical Principle
- 4 Reagents and materials
- 4.4 chlorine disinfectant
- 4.5 agarose
1 Scope
This section GB/T 28630 specifies the nested PCR for detection of White Spot Syndrome pathogen required reagents and materials, equipment,
Procedure and result judgment.
This section applies to a variety of samples of shrimp, a variety of biological and environmental samples of food organisms, and various other non-biological samples for
White Spot Syndrome pathogens with high sensitivity qualitative detection of infected cases, for pathogen screening and diagnosis of diseases.
When used alone in this section do not apply to activity estimation and evaluation of the degree of host infected with the virus or the amount of infection.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. Who does not note date reference documents, the latest edition (including any amendments) applies to this document.
Diagnostic protocols GB/T 28630.4-2012 spot syndrome (WSD) Part 4. Histopathological diagnosis method
Principle 3
Pathogens and pathological features of White Spot Syndrome 3.1
See GB/T 28630.4-2012 2.1.
3.2 Technical Principle
Polymerase chain reaction (polymerasechainreaction, PCR) is a DNA sample to be tested as a template, with the template
A pair of specific oligonucleotide sequences complementary to both ends of the sequence as a primer, in the presence of four kinds of deoxyribonucleoside triphosphates, use-dependent
DNA synthesis of DNA polymerase. After dozens of denaturation, annealing and extension reaction cycle, so that between the two primers on the template
DNA fragment obtained specifically between the amplification stages, is specifically amplified fragment was detected by electrophoresis and other means, which can reveal
Trace viral DNA. Nested PCR is the first step in the PCR amplified product, and then a pair of primers second step PCR amplification increase. Nested PCR reactions can be greatly increased sensitivity and specificity of PCR detection.
4 Reagents and materials
4.1
Unless otherwise stated in the analysis using only the confirmation of analytical grade chemicals and distilled or deionized water or equivalent purity.
4.2
ethanol.
4.3
liquid paraffin.
4.4 chlorine disinfectant
medical supplies or aquatic drugs.
4.5 agarose
electrophoresis grade.
......
This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 15 pages — is available in the English PDF.
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Related Standards
GB/T 28630.3-2012 — Diagnostic protocols for white spot disease - Part 3: In situ hybridization method
GB/T 28630.1-2012 — Diagnostic protocols for white spot disease - Part 1: Dot blot hybridization method with DNA probe
GB/T 28630.4-2012 — Diagnostic protocols for white spot disease - Part 4: Histopathology method
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