GB/T 28630.1-2012Diagnostic protocols for white spot disease - Part 1: Dot blot hybridization method with DNA probe (English PDF)
白斑综合征(WSD)诊断规程 第1部分：核酸探针斑点杂交检测法
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Issued by
SAMR; SAC
Level / Type
National · Recommended
Issue date
July 31, 2012
Implementation date
November 1, 2012
Scope
GB/T 28630.1-2012 is the English-translated version of 白斑综合征(WSD)诊断规程 第1部分：核酸探针斑点杂交检测法.
GB/T 28630.1-2012 is the Chinese standard "Diagnostic protocols for white spot disease - Part 1: Dot blot hybridization method with DNA probe". Its scope clause reads: This section GB/T 28630 provisions of the White Spot Syndrome DNA probe dot blot hybridization methods needed reagents and materials, equipment, Procedure and result judgment.
This section applies to shrimp as shrimp, young shrimp, shrimp larvae, juveniles and fertilized in vivo, chilled or frozen products and other white spot syndrome Virus sensitive crustaceans White Spot Syndrome pathogen screening, clinical diagnosis of the condition, the estimate does not apply to active viral infection. Its clauses include reagents and materials.
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Document preview — GB/T 28630.1-2012
National Standard of the People's Republic of China
- ICS
- 65.020.30
- Classification
- B 41
Issued by: State Administration for Market Regulation; Standardization Administration of the PRC
Contents
- 1 Scope
- 2 Normative references
- 3.2 Technical Principle
- 4 Reagents and materials
- 4.2 chlorine disinfectant
- 4.3 Hybrid Membrane
- 4.5 Sodium lauryl sarcosine (Sarkosyl)
- 4.6 sodium dodecyl sulfate (SDS)
- 4.7 disodium edetate (EDTA-Na2 · 2H2O)
- 4.8 mercaptoethanol
- 4.9 Tris balance phenol (saturated phenol)
1 Scope
This section GB/T 28630 provisions of the White Spot Syndrome DNA probe dot blot hybridization methods needed reagents and materials, equipment,
Procedure and result judgment.
This section applies to shrimp as shrimp, young shrimp, shrimp larvae, juveniles and fertilized in vivo, chilled or frozen products and other white spot syndrome
Virus sensitive crustaceans White Spot Syndrome pathogen screening, clinical diagnosis of the condition, the estimate does not apply to active viral infection.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated edition applies to this article
Member. Where not dated references, the latest edition (including any amendments) applies to this document.
Diagnostic protocols GB/T 28630.4-2012 spot syndrome (WSD) Part 4. Histopathological diagnosis method
Principle 3
Pathogens and pathological features of White Spot Syndrome 3.1
See GB/T 28630.4-2012 2.1.
3.2 Technical Principle
In non-radioactive marker --- digoxigenin labeled White Spot Syndrome Virus (WSSV) DNA fragment syndrome, or nucleic acid probes, and present in the
Samples of viral DNA specificity of nucleic acid hybridization, by an antigen - antibody reaction and enzyme - substrate chemical color reaction like display
Products in the presence of a virus.
4 Reagents and materials
4.1
Unless otherwise stated in the analysis using only recognized as analytical grade reagents and distilled or deionized water or equivalent purity.
4.2 chlorine disinfectant
medical supplies or aquatic drugs.
4.3 Hybrid Membrane
nitrocellulose membrane.
4.4
PCRDIGProbeSynthesisKit1).
4.5 Sodium lauryl sarcosine (Sarkosyl)
chemical reagents.
4.6 sodium dodecyl sulfate (SDS)
chemical reagents.
4.7 disodium edetate (EDTA-Na2 · 2H2O)
chemical reagents.
4.8 mercaptoethanol
biochemical reagents.
4.9 Tris balance phenol (saturated phenol)
biochemical reagents.
1) PCRDIGProbeSynthesisKit is provided by Roche, the trade name of the product. This information is given in order to facilitate the use of this section
Who does not imply endorsement of the product. If other equivalent product has the same effect, you can use these equivalent products.
......
This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 15 pages — is available in the English PDF.
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Related Standards
GB/T 28630.2-2012 — Diagnostic protocols for white spot disease - Part 2: Nested PCR method
GB/T 28630.3-2012 — Diagnostic protocols for white spot disease - Part 3: In situ hybridization method
GB/T 28630.4-2012 — Diagnostic protocols for white spot disease - Part 4: Histopathology method
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GB/T 28630.1-2012
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