GB/T 40255-2021Code of diagnosis for infection with hepatopancreatic parvovirus of penaeid shrimp—PCR method (English PDF)
对虾肝胰腺细小病毒病诊断规程 PCR检测法
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Issued by
SAMR; SAC
Level / Type
National · Recommended
Issue date
May 21, 2021
Implementation date
December 1, 2021
Scope
GB/T 40255-2021 is the English-translated version of 对虾肝胰腺细小病毒病诊断规程 PCR检测法.
GB/T 40255-2021 is the Chinese national diagnostic protocol for detecting infection of penaeid shrimp with hepatopancreatic parvovirus (HPV) by the polymerase chain reaction.
It describes a nucleic acid test that can be used within the diagnosis of the disease, listing the reagents and materials, the equipment, the samples, the test steps and how the result is judged.
The method is qualitative and can be applied to shrimp at every life stage, for epidemiological investigation, diagnosis, quarantine and monitoring. The standard lists the abbreviations used in the procedure, from DNA and dNTPs to EDTA, SDS, Taq polymerase and TE buffer, and refers to the histopathological method of GB/T 28630.4. It was issued on 21 May 2021 and has applied since 1 December 2021.
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Document preview — GB/T 40255-2021
National Standard of the People's Republic of China
- ICS
- 65.020.30
- Classification
- B 41
Issued by: State Administration for Market Regulation; Standardization Administration of the PRC
Contents
- Foreword
- 1 Scope
- 2 Normative references
- 3 Terms and definitions
- 4 Abbreviations
Foreword
This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for Standardization Work Part 1: Structure and Drafting Rules of Standardization Documents" Drafting.
1 Scope
This document specifies the requirements for nucleic acid detection techniques that are generally applicable in the diagnostic procedures for shrimp hepatopancreatic parvovirus disease.
Hepatopancreaticparvovirus (HPV), which provides the reagents or materials, equipment, samples, test Test steps and result determination.
This document is applicable to the qualitative detection of HPV infection in shrimp samples at various life stages for the purpose of detecting shrimp hepatopancreatic parvovirus disease. Epidemiological investigation, diagnosis, quarantine and monitoring.
2 Normative references
The contents of the following documents constitute the indispensable clauses of this document through normative references in the text. Among them, dated quotations Only the version corresponding to that date is applicable to this document; for undated reference documents, the latest version (including all amendments) is applicable to this document.
GB/T 28630.4-2012 White Spot Syndrome (WSD) Diagnostic Procedure Part 4: Histopathological Diagnostic Method
3 Terms and definitions
There are no terms and definitions that need to be defined in this document.
4 Abbreviations
The following abbreviations apply to this document.
bp. base pair (basepair)
DNA. deoxyribonucleic acid (deoxyribonucleicacid)
dNTPs. deoxy-ribonucleosidetriphosphate
EB. Ethidium bromide (ethidium bromide)
EDTA. ethylenediaminetetraacetic acid (ethylenediaminetetraacetic acid)
HCl. Hydrochloride (hydrogenchloride)
HPV. hepatopancreaticparvovirus
NaOH. sodium hydroxide (sodiumhydroxide)
PCR. polymerase chain reaction (polymerase chain reaction)
SDS. Sodiumdodecylsulfate (sodiumdodecylsulfate)
Taq. Thermus aquaticus (thermusaquaticus)
TE. Tris hydrochloric acid and EDTA buffer (EDTATris·HCl)
Tris. trishydroxymethylaminomethane (trishydroxymethylaminomethane)
......
This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 15 pages — is available in the English PDF.
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