GB/T 40257-2021Code of diagnosis for infection with taura syndrome virus—RT-PCR method (English PDF)

Foreword

This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for Standardization Work Part 1: Structure and Drafting Rules of Standardization Documents" Drafting.

1 Scope

This document specifies the requirements of the universally applicable nucleic acid detection technology in the diagnostic procedures for Taura syndrome, and is aimed at Taura syndrome virus. (taurasyndromevirus, TSV), which provides reagents or materials, equipment, samples, test procedures and The result is judged.

This document is applicable to the qualitative detection of TSV virulence in shrimp samples at various life stages for the epidemiological investigation of Taura syndrome. Inspection, diagnosis, quarantine and monitoring.

2 Normative references

The contents of the following documents constitute the indispensable clauses of this document through normative references in the text. Among them, dated quotations Only the version corresponding to that date is applicable to this document; for undated reference documents, the latest version (including all amendments) is applicable to this document.

GB/T 28630.4-2012 White Spot Syndrome (WSD) Diagnostic Procedure Part 4: Histopathological Diagnostic Method

3 Terms and definitions

There are no terms and definitions that need to be defined in this document.

4 Abbreviations

The following abbreviations apply to this document.

bp. base pair (basepair)

cDNA. complementary deoxyribonucleic acid (complementaryDNA)

DEPC. Diethyl pyrocarbonate (diethypyrocarbonate)

DNA. deoxyribonucleic acid (deoxyribonucleicacid)

dNTPs. deoxy-ribonucleosidetriphosphate

EB. Ethidium bromide (ethidium bromide)

EDTA. ethylenediaminetetraacetic acid (ethylenediaminetetraacetic acid)

M-MLV. Moloney Murine Leukemia Virus (moloney-murineleukeminvirus)

PCR. polymerase chain reaction (polymerase chain reaction)

RNA. ribonucleic acid (ribonucleicacid)

RT-PCR. reverse transcription-polymerase chain reaction (reversetranscription-polymerasechain reaction)

Taq. Thermus aquaticus (thermusaquaticus)

Tris. trishydroxymethylaminomethane (trishydroxymethylaminomethane)

TSV. taurasyndromevirus