GB/T 21675-2022Diagnostic techniques for African horse sickness (English PDF)

Foreword

This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for Standardization Work Part 1: Structure and Drafting Rules for Standardization Documents" drafting.

This document replaces GB/T 21675-2008 "African Horse Sickness Diagnostic Technique". Compared with GB/T 21675-2008, except for structural adjustment and Apart from editorial changes, the main technical changes are as follows.

--- Added the chapter "Abbreviations" (see Chapter 4);

--- Added "biosecurity measures" (see Chapter 5);

--- Added "General Real-timeRT-PCR method" (see 8.3);

--- Added "type-specific Real-timeRT-PCR method" (see 8.4);

--- Added "blocking ELISA antibody detection method" (see 8.6);

--- Deleted "separation of suckling mice" (see 4.1.4 of the 2008 edition);

--- Deleted "micro-complement fixation reaction" (see 4.2.2 of the 2008 edition).

Introduction

A non-contact viral infectious disease caused by equine animals, characterized by changes in the respiratory system and circulatory system, often causing horses, mules At least two species of Culicoides can spread the disease. AHSV belongs to the genus Orbivirus of the family Reoviridae. The disease is currently known to have 9 serotypes type. The World Organization for Animal Health (WOAH) lists it as a notifiable animal disease. my country defines it as a first-class animal disease.

The occurrence of this disease has a certain seasonality and regionality, and it is more common in hot and humid seasons. Once it is introduced, it is often endemic or outbreaks. broadcast quickly. Thick frost, high dry terrain, natural barriers and other climatic and geographical conditions that affect the reproduction or activities of vector insects can significantly reduce the disease.

The content of diagnostic techniques in this document includes clinical diagnosis, etiological diagnosis methods and serological diagnosis methods.

The revision of this document refers to the WOAH Manual of Diagnostic Tests and Vaccines for Terrestrial Animals (2019), and incorporates relevant Chinese technologies.

1 Scope

This document specifies the clinical diagnosis of African horse sickness, sample collection and processing, as well as virus isolation and identification, RT-PCR, general-purpose Real-timeRT-PCR, type-specific Real-timeRT-PCR, indirect ELISA antibody detection, blocking ELISA antibody detection and other experiments Technical requirements for laboratory diagnostic methods.

This document is applicable to the diagnosis, detection, quarantine, surveillance and epidemiological investigation of AHS in horses, mules, donkeys and other equines.

2 Normative references

The content in the following documents constitutes the essential provisions of this document through normative references in the text, among which, the dated references For documents, only the version corresponding to the date is applicable to this document; for undated reference documents, the latest version (including all amendments) is applicable to this document.

GB 19489 General Requirements for Laboratory Biosafety

3 Terms and Definitions

This document does not have terms and definitions that need to be defined.

4 Abbreviations

The following abbreviations apply to this document.

ABTS: 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (2,2'-azino-di-[3-ethyl-benzothiazoline]-6- sulphonic acid)

CPE: cytopathic effect (cytopathic effect)

Ct: cycle threshold (cyclethreshold)

Cy5: cyanine fluorescent dye (cyanine5)

FAM: 6-carboxyfluorescein (6-carboxy-fluorescein)

MGB: DNA minor groove binder (minorgroovebinder)