GB 7413-2009Quarantine protocol for propagating tubers and seedlings of sweet potato in producing areas (English PDF)

Foreword

All the technical contents of this standard is mandatory. This standard replaces GB 7413-1987 "potato seedlings quarantine rules." This standard compared with GB 7413-1987, the main changes are as follows:

--- Modify the standard English name, standard structure, some of the terms;

--- Sweet potato seedlings quarantine procedures, detection and investigation of its aspects was handling the outbreak supplementary provisions. The Standard Appendix A, Appendix D, Appendix E, Appendix G and Appendix H of normative appendix, Appendix B, Appendix C and Appendix F for the data Appendix. This standard by the National Standardization Technical Committee on plant quarantine and centralized. This standard was drafted. National Agricultural Technology Extension Service Center, Anhui Province plant protection station. The main drafters of this standard. Item Yu, Huang Chao, WU Li-feng, Zhu Jing whole, Julie. This standard replaces the standards previously issued as follows:

--- GB 7413-1987. Sweet potato seedlings quarantine rules

1 Scope

GB 7413-2009 is the Chinese national standard on quarantine protocol for propagating tubers and seedlings of sweet potato in producing areas, in the field of agriculture. It carries no /T suffix, which in the Chinese system means compliance is mandatory: a product or a practice within its scope has to meet it to be lawfully made, sold or carried out in China. It was issued on 27 April 2009 by the General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China; Standardization Administration of China, and has been in force since 1 October 2009. Classification: ICS 65.020.01, CCS B16. This page is published from the official record of the standard held by the Chinese standards administration: the identification, the dates, the classification and the issuing body are taken from there. The clause text, the tables and the numeric limits are in the document itself, which is delivered complete in English translation.

This standard specifies the procedures and methods of potato seedlings quarantine. This standard applies to agricultural plant quarantine agencies to implement quarantine potato seedlings.

2 Terms and definitions

The following terms and definitions apply to this standard.

2.1 Plant quarantine bodies of plants and their products (including seeds and other propagating material) quarantine all work in the production process of the country of origin, including Including field survey, indoor testing, issuing certificates and supervise the production units to do the election, the selection and disease treatment and so on.

2.2 Plants, plant products or other regulated articles official visual inspection to determine if pests are present, compliance with phytosanitary Regulations.

2.3 To determine if pests are present or in addition to the visual inspection for the official identification of the pest carried out.

2.4 For areas threatened by its potential economic importance, but not in place in the region, or, having incurred but not widely distributed and official proof Pest governance.

2.5 Sweet potato tubers and potato seedlings.

3 should quarantine pests

3.1 administrative department of agriculture of the State Council published the National Agricultural plant quarantine pests.

3.2 provincial administrative department of agriculture announced supplementary agricultural plant quarantine pests.

4 quarantine accepted

Phytosanitary audit institutions and individuals potato seed production unit's "quarantine applications" (see Appendix A) and the provision of related Materials, to decide whether to accept it.

5 Prepare and technical guidance

Phytosanitary quarantine agencies to develop plans and potato seed breeding base quarantine guidance, sweet potato seedling production parameters prevention measures See Appendix B.

8 Visas

8.1 After field, indoor inspection found no quarantine pests of seedlings, plant quarantine agencies to issue "quarantine certificate" (see Annex Record H). "Quarantine certificate" valid for 1 year.

8.2 found quarantine pests, quarantine insecticide treatment shall be issued "quarantine certificate"; quarantine insecticide treatment failure And not the issue of "quarantine certificate" and inform the applicant or quarantine individuals.

9 File Management

Raw survey data in quarantine process, forms, samples and other data files you want to keep, save time less than 2 years.

Appendix A

(Normative) Quarantine application Table A. 1 quarantine application ID. The date Plant Names Species name Species (Miao) Source Production area It is expected to yield Production sites Production for the period from expiring Applicant Name (stamp). Address. Zip. Contact (Signed). Tel. Fax. Requirements for this document sending method Come to collect Express mail Standard Delivery

Appendix B

(Informative) Sweet Potato seed production prevention measures B. 1 seed to selection B.

1.1 seedlings to be elected in the region free from quarantine pests occur. Selection of more than 3 years not planted to sweet potatoes as a disease-free seed Tian, strict selection, seedling selection. B.

1.2 There shall be selected from the outbreak area in isolation conditions (not potato species within around 1km range, seedlings or ground irrigation system on independence Tour without quarantine pests) plots. B. 2 seed selection B.

2.1 Seed collected from non-quarantine pests region occur. B.

2.2 The nursery of seedlings one by one check, choose healthy seedlings. Suspicious diseased (block) indoor identification. B. 3 seed to prevention measures B.

3.1 prohibited without quarantine seedlings seedlings into the ground. B.

3.2 non-outbreak areas pigs, cattle, sheep and farmyard manure into the ground seed. B.

3.3 seedlings to the tools dedicated to new home, the person responsible. B.

3.4 seedlings occur require timely pest control. B. 4 seed pit entry management B.

4.1 seed single income, single place, a single pit storage. B.

4.2 before seed disinfection pit pit entry response. B.

4.3 strictly check the selection of disease-free seed potatoes, dry pit entry. B.

4.4 Storage of secondary person responsible for the management.

Appendix C

(Informative) Field stem rot nematode symptoms C. 1 seedling symptoms Seedbed emergence little yellow dwarf, the white part of the stem base seedlings appear mottled, after changes to black, there is a gap in the stem cut open, brown or pith Purple, broken little stream does not flow or white pulp. C.

2 Field Period Symptoms Early symptoms of growth, seedling vine short mid performance, near-surface cracks potato Shui epidermis, leaf yellowing from the bottom up, these disorders Like the victim is caused by the underground tubers. C. Tuber symptoms on

3 There are three common types. One is bran heart-shaped, from the onset of tuber size, color and other aspects of normal potato no significant difference between the epidermis Intact, but the interior due to the tubers after stimulation by the nematode, thin-walled cell dehydration, shrinkage white spongy (or powder), a large gap, called "Bran Heart." Since the latter part of the soil bacteria opportunistic infections, therefore rendering brown and white heart-shaped bran (severe skin was dark brown - liver color). This type is more direct infection caused by the nematode with seedlings; the other is split skin type, tuber skin cracking, dehydration shrinkage. This class Type directly from the soil nematode infestation caused by sucking; third type is mixed, the performance of the internal hollowing, crack external skin.

Appendix D

(Normative) Quarantine field survey record sheet Table D. 1 quarantine field survey record sheet Computer File ID. Survey Location Plant Names cultivar name Survey date plant growth period Approved acreage yield Chinese name Latin name Area sampling area happened Sample number of trees number of trees killed The outbreak situation Plots name survey unit survey Quantity Instars disease classification classification count count (the highest grade) Egg larvae pupa adult 012 345 Subtotal Quarantine agencies Conclusion The epidemic recorder (Signed) Party (Signed) inspectors (Signed) Remark

Appendix E

(Normative) Pest survey sampling record sheet Table E. 1 pest survey sampling record sheet Numbering. Production/operator address and zip code Contact/contact person in charge phone Survey Sampling Location Date sample Numbering Botanical name (Chinese name and scientific name) Variety name plant Growth Period Investigation on behalf of The number or area plant source Descriptions of the symptoms. Occurrence and Prevention and the reasons. Sampling methods, location and sampling ratio. Remarks. Phytosanitary Authority (stamp). Preparer (Signed). year month day Production/operator Site person in charge year month day

Note. This unitary two together, the first joint plant quarantine agencies filing, second copy is examined units.

Appendix F

(Informative) Stem rot nematode indoor test methods F. Sample

1 In potato breeding period, we found seedbed sparse seedlings growing weak, even rotten yellow dwarf seedlings showing symptoms should check each tree seedlings, seedling disease and will be Rhizosphere soil taken back to the laboratory for nematode Isolation and identification. During the field, in the sweet potatoes and cut dry matter yield planing, the disease is the best time of the survey Of collecting tubers showing symptoms, back to the laboratory for nematode Isolation and identification. F. Save 2 samples The collected samples back to the lab separated in time, if not separated in time, samples can be stored in a refrigerator 4 °C ~ 10 °C in. F. 3 separate samples nematodes F.

3.1 Baermann funnel method. selection of the diameter of 10cm ~ 14cm glass funnel, and then the following section of latex tubing, latex tube tops a seal Clip, filled with water in the funnel, the funnel placed on the shelves, the chopped plant material or soil sample with a layer 2 to 3 layers of gauze or high toughness Grade tissue wrap gently on the funnel, after 24h ~ 48h, nematodes due to its water-based and tend to sink under its own weight of milk under the hopper The hose with a small petri dish or test tube bonding under latex tube, release the clip seal collected nematode suspension. F.

3.2 platter funnel method. the sample is placed covered with 2 layers of gauze or tissue of small sieve tray, and then put into a small sieve tray filled with water funnel The other procedures were the same Berman funnel method. Small sieve tray diameter smaller than the diameter of the funnel 2cm ~ 3cm, depth of 2cm, mesh diameter of 0.2cm ~ 0.5cm. F.

3.3 shallow basin method. The sample was laid flat on a 2-layer gauze or tissue sieve plate, sieve bowl on the amount of water containing the bottom of the basin, Just soak in water samples is appropriate, after 24h ~ 48h, remove the bowl screen, the bottom of the pot nematode suspension was passed through a 400 mesh (pore size 38µm) of Sieve collected nematodes. F. 4 examination Separation of the resultant suspension of nematodes in a test tube and placed in water bath 60 °C ~ 65 °C in 2min ~ 3min kill nematodes. Already Kill nematodes promptly fixed with 4% formaldehyde, made into slides under the microscope morphological nematode specimens were observed and measured, and the rot Morphological characteristics rotten stem nematode comparison, if the match is determined nematode identified as D. destructor. F.

5.1 Form Female. parasites linear, hot kill parasites bent slightly to the ventral, lateral line 6. Head flat, slightly constricted, obviously stylet base ball, in Esophagus ball spindle with flap, back covering after esophageal intestinal glands short (and occasionally constricted). Single ovary, reach, sometimes extends up to the esophagus area, after Yin child Palace anal sac length is from 40% to 98% of the female pitch. Tail cone, usually curved belly, side round. Male. the front and tail shape of the female body shape. Bursa extends 50% to 90% of the tail, spicules length 24µm ~ 27µm. Female. L = 0.69mm ~ 1.89mm; Rao = 18 to 49; Chou = 4 to 12; Ba = 14 to 20; Ba '= 3-5; Vp = 77 to 84; mouth of the needle length = 10µm ~ 13µm. Male. L = 0.63mm ~ 1.35mm; Rao = 24 to 50; Chou = 4 to 11; Ba = 11 to 21; stylet length = 10µm ~ 12µm. Major surveying projects (DeMan formula). L

--- body length; Rao

--- length/maximum body width; Chou

--- Length/distance from the front end of the esophagus to the end; Vp front end

--- the distance vulva × 100/body length; Ba

--- body length/tail length; Ba '

--- tail length/width anus body. F. 6 equipment required Microscope 1; dissecting microscope 1 set; funnel 1; Iron frame 1; a shallow dish; sieve group; a bottom basin; latex tube 1; seal Clip one; several tubes; a plurality of dish; gauze 2; pick a needle. F. 7 fixative formulation Standard formaldehyde fixative. Formalin (40% formaldehyde). distilled water = 1.9. Double glycerol formaldehyde fixative. Formalin (40% formaldehyde). distilled water = 2.8.

Appendix G

(Normative) Pest Identification Report Sample Table G.

1 Pest Identification Report Sample Numbering. Plant Names cultivar name Plant growth of the number of samples Sampling site Source sample submission date inspection person Tel inspection unit Detection and identification methods. Detection and identification results. Remarks. Appraiser (Signed). Approved by (signature). Identification Seal. year month day

Note. This triplicate testing unit, examined units and quarantine agencies each.

Appendix H

(Normative) Quarantine certificate Table H. 1 quarantine certificate Numbering. Plant variety name or product name Number Area Place of origin Production unit or head of household contacts Address Phone Quarantine Results. Inspectors (Signed). year month day Plant quarantine agencies validation opinion Phytosanitary Agency (quarantine seal) year month day NOTE. This permit is valid for 1 year and keep it, is not transferable, to be transported to the plant or product, by virtue of permits for the "phytosanitary certificate to the phytosanitary agency book". Quarantine certificate (stub) Numbering. Plant variety name or product name Number Area Place of origin Production unit or head of household contacts Address Phone Quarantine Results. Inspectors (Signed). year month day Plant quarantine agencies validation opinion Phytosanitary Agency (quarantine seal) year month day