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GB 7411-2009Quarantine protocol for cotton seeds in producing areas (English PDF)

棉花种子产地检疫规程

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Issued by

General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China; Standardization Administration of China

Level / Type

National · Mandatory

Issue date

April 27, 2009

Implementation date

October 1, 2009

Scope

GB 7411-2009 is the English-translated version of 棉花种子产地检疫规程.

GB 7411-2009 is the Chinese national standard on quarantine protocol for cotton seeds in producing areas, in the field of agriculture. It carries no /T suffix, which in the Chinese system means compliance is mandatory: a product or a practice within its scope has to meet it to be lawfully made, sold or carried out in China. It was issued on 27 April 2009 by the General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China; Standardization Administration of China, and has been in force since 1 October 2009. Classification: ICS 65.020.01, CCS B16. This page is published from the official record of the standard held by the Chinese standards administration: the identification, the dates, the classification and the issuing body are taken from there. The clause text, the tables and the numeric limits are in the document itself, which is delivered complete in English translation.

Document preview — GB 7411-2009

National Standard of the People's Republic of China

ICS: 65.020.01
Classification: B16

Issued by: General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China; Standardization Administration of China

1 Scope
1.1 Mechanical cashmere
2 Terms and definitions
5 Application processing
6 Investigation Detection
6.1 Field Survey
9 Visa
Appendix A
Appendix B
Appendix C
Appendix D
Appendix E
Appendix F
Appendix G
Appendix H

Foreword

All the technical contents of this standard is mandatory. This standard replaces GB 7411-1987 "raw cotton (good) kind of quarantine rules." This standard and GB 7411-1987 compared to the main changes are as follows:

--- Revised standard, the English name, standard structure, some of the terms;

--- Of cotton seed quarantine procedures, investigation aspects of its testing, monitoring and epidemic indoor treatment was supplemented by regulations. The standard Appendix C, Appendix D, Appendix H are normative appendices, Appendix A, Appendix B, Appendix E, Appendix F, Appendix G for information only appendix. This standard by the National Standardization Technical Committee on plant quarantine and centralized. This standard was drafted. National Agricultural Technology Extension Service Center, Plant Protection Station of Hubei Province. The main drafters of this standard. Wang Yuxi, Xu Hong, Liu Hui, Liu Yuanming, Julie. This standard replaces the standards previously issued as follows:

--- GB 7411-1987. Cotton seed quarantine rules

1 Scope

This standard specifies the procedures and methods of cotton seed quarantine. This standard applies to plant quarantine agencies at all levels of cotton seed breeding base for the implementation of quarantine.

1.1 Mechanical cashmere

--- Foam acid cottonseed lint technique. After dilution of concentrated sulfuric acid to 8% to 10% concentration, according to the amount of sulfuric acid down ratio 1.6, according to the Cottonseed containing the level of cashmere rate, adding a certain amount of foaming agent and fully stirred, under the action of compressed air, so that the mixture bubble Mo-based, relying on capillary action lint absorption acid.

--- Cottonseed lint technical sulfuric acid. dilute sulfuric acid is diluted sulfuric acid from cashmere to a concentration of 8% to 10%, the amount of added activator, The cottonseed with dilute sulfuric acid solution in proportion

3.1 around, soak in a stirred tank agitated to cottonseed all wet, and then Centrifuge to separate the excess acid for reuse; and the attachment of cottonseed linters sulfuric acid was only about 10%. The foam mixed acid (or dilute sulfuric acid was mixed with) the cottonseed after drying, sulfuric acid in water evaporates, the concentration of sulfuric acid increased, Carbide surface cottonseed short fiber; through intense friction friction inside the machine, worn surface carbonation cottonseed short fiber, seeds become light. Light cashmere seed after selection and can be mixed with the drug coating. G.

1.2 manual from cashmere 90% of the industrial concentrated sulfuric acid per 500g detachable cottonseed 2.5kg ~ 3.5kg. Cottonseed hot sun after the first five times the volume of cotton in earthenware container (jar, pot, etc.) inside, then slowly pour the appropriate amount of sulfuric acid, while Down while mixing until all the fuzz is dissolved sulfate (burned), cotton was glossy black to the general cashmere no more than 15min. After cashmere Immediately with plenty of water to rinse the seeds until the rinse water was yellow, not sour (no sour tongue licking, tongue Ma does not prevail). Seeds sown directly after washing or dry stalls after sowing. G. 2 cottonseed pharmaceutical treatment

--- Dressing Methods. 50% carbendazim WP, 70% PCNB or wettable powders, or 70% thiophanate Wettable powder, 0.8% by weight of cotton seed amount of dressing;

--- Soaking. selection of 50% carbendazim wettable powder 25g ~ 50g or 50% of the enemy Nixon WP 20g, water 400g ~ Rub mixed with 500g cotton 500g, dry after planting. Or 5% of water poisoning bacteria agent 300 times to 500 times soaking 24h, fish Dry direct seeding;

--- "402" Temperature of Soaking law. first quantitative hot water into the tank, and the water temperature is adjusted to about 65 °C, then poured into a predetermined concentration "402" liquid, stir, and finally into the sulfated lint cottonseed, cottonseed per 100kg with 250kg ~ 300kg dry liquid (Amount of liquid is

2.5 times to 3 times the amount of cottonseed). Sacks or wooden lid seal, hot water dip liquid were bored species. In the immersion process stuffy To be stirred for 2 to 3 times, the upper and lower temperature consistent, always keep the water temperature between 55 °C ~ 60 °C, soaked for half an hour that is kind of boring fishing Out. May vary with the seeding process, you can also remove and spread to dry in the sun field use. G. 3 disease point Soil disinfection technology During the cotton growing or post-harvest cotton flower before pulling the lever, pick up the sick deformed and diseased plants around the net, topsoil (1.67cm ~ 3.34cm deep) collected Disease center point, and the center point of the disease process calibration 1m2 points around as a ridge of soil within 0cm ~ 30cm soil scarification (conditional poached Soil disease), carried out before winter pharmaceutical treatment, chemical treatment method is.

--- Dazomet treatment. per square meter (40cm deep) put 50% or wettable powder 140g original powder 70g, and turn loose soil Hunban uniform, then 15kg ~ 25kg water Aided. And with dry fine soil tightly closed Disease point;

--- Chloropicrin treatment. cotton bud stage, spraying per square meter, the first point in the disease three to five drilling hole depth 20cm, pitch strain 20cm, each with a straw hole injection 10mL; the blooming period, per square meter drilling 9 to 12, each hole is still injection 10mL, then Opening closed with soil;

--- Farm aqueous ammonia. ammonia 16% of agricultural ammonia

1.9 times per square meter 45kg.

2 Terms and definitions

The following terms and definitions apply to this standard.

2.1 Plants, plant products or other regulated articles official visual inspection to determine if pests are present, compliance with phytosanitary Regulations.

2.2 To determine if pests are present or in addition to the visual inspection for the official identification of the pest carried out.

2.3 For areas threatened by its potential economic importance, but not in place in the region, or, having incurred but not widely distributed and official proof Pest governance.

3 should quarantine pests

3.1 administrative department of agriculture of the State Council published the National Agricultural plant quarantine pests.

3.2 provincial administrative department of agriculture announced supplementary agricultural plant quarantine pests. Principle

4 Morphological characteristics of quarantine pests in cotton fields and its host range, geographical distribution, biological characteristics, damage symptoms and transmission (cf. See Appendix A) is the standard scientific basis.

5 Application processing

5.1 Site acceptance According to cotton seed production units and individuals to apply, according to conventional census and survey results, the site of origin to decide whether to issue a qualified quarantine It proved that seed production quarantine supervision and guidance (see Appendix B).

5.2 quarantine accepted Phytosanitary agency audit cotton seed production units and individuals of the application and related materials provided to decide whether to accept it. Decide Accepted phytosanitary quarantine agencies to develop plans.

6.1 Field Survey

6.1.1 investigation time According quarantine pests occurrence, cotton growing and climatic conditions of the investigation to determine the time and frequency. Verticillium generally squaring flowering and boll stage Lianyin rain 7d ~ 10d, respectively, the survey 1, increase the number of surveys where necessary.

6.1.2 Survey Methods Visual survey of the whole cotton seed production base (point), to determine the sampling field. Each species sampled area of not less than the total local Breeding 10% of the area. Verticillium targeted selection of low-lying, easy to water, even for the cotton fields as a sample survey fields. The method uses a parallel jump sampling interval of 4 1 line survey line survey Tanabe left five set points continuous survey 20 points per interval 30 ~ 50 and then take the second point of the investigation. Every field of sampling points is not less than 5.00, the total number of trees less than 100 investigations.

6.2 Field Inspection Conduct on-site field according to a quarantine pest hazards like preliminary inspection to quarantine pests and visual field damage characteristic morphology Characteristics as the basis, can be directly identified, will fill in the results "pest survey sampling record sheet" (see Appendix C) and "harmful biological sample KAM Set Report "(see Appendix D). Suspicious keep pests after sampling, sample records cultivar name on the sample bag, planting location, acquisition time, picking up a person, as Indoor testing. Verticillium in accordance with Appendix A of A.

2.1 symptoms described investigation, dwarf, deciduous and other cotton Verticillium suspected Choice Three to sixth disc is not broken or woody fruiting petiole observation, in line with the symptoms described cotton count, enter "pest survey sampling record Table "(see Appendix C). Load sample bag suspected of cotton, properly preserved. Variety described the sample name on the sample bag, planting location, acquisition time Room gatherers.

6.3 Save the image field Conditional shot quarantine sub-species of the object field panorama, suspected diseased photographs, close-ups of symptoms save.

7 indoor testing

7.1 Detection Methods Suspicious pest identification methods in accordance with the appropriate indoor identification, according to standard criteria, there is no standard according to the target Pest feature selection conventional detection methods. Verticillium suspected diseased plants, in accordance with Annex A, Appendix E of the method of vascular discoloration of cotton were cultured and identified.

7.2 Result judgment Results quarantine judgment based on test results and laboratory test results to identify filled with "harmful biological sample appraisal report" (see Appendix D) and "quarantine inspection results notice." If necessary, preserved specimens (see Appendix F).

8 disease treatment

After field investigation found quarantine pests, guiding production units and individuals to implement quarantine treatment. For cotton verticillium wilt disease field seeds can be carried out under the supervision of plant quarantine department after seed disinfection (see Appendix G), limit Use the outbreak in the region can not be transferred to non-endemic area.

9 Visa

9.1 or indoor testing through field investigation, found no quarantine pests of cotton production base (point), the issue of "quarantine certificate" (see Appendix H).

9.2 found quarantine pests, quarantine insecticide treatment shall be issued "quarantine certificate"; quarantine insecticide treatment failure And not the issue of "quarantine certificate" and inform the applicant or quarantine individuals.

10 File Management For raw survey data in quarantine work, tables, and other data files properly preserved specimens, save time less than 2 years.

Appendix A

(Informative) Verticillium characteristic symptoms and biological characteristics A.

1 Classification and nomenclature A.

1.1 Verticillium name.

--- Chinese name. Verticillium;

--- English name. Verticiliumwiltofcotton; A.

1.2 Verticillium genus of imperfect fungi Amon (Deuteromycotina), Trichosporon Gang (Hyphomycetes), Trichosporon mesh (Hyphomyce- A. 2 field symptoms and morphological characteristics of the pathogen A.

2.1 Field symptoms Cotton throughout the growing period can be victims, field general onset after budding, blooming period reached the peak incidence of diseased plants wilting leaves withered. Common symptoms can be divided into the following types.

a) macula type. Plant basal leaves show symptoms first, and gradually up the Department for Development. The incidence of diseased leaves margin slightly up volume, between the main vein Now yellow patches, irregular shape, after expanding lesion, deeper color, but close to the main vein and leaf remains green, like a watermelon, Diseased leaves do not fall off;

b) type of leaf blight. Diseased leaves have brown spots or localized dry spot blight palmate leaves withered after shedding, but generally do not form a light rod;

c) acute wilting type. In the boll period, acute symptoms, between the blades of the main vein green soaked chlorotic spots, sagging after heavy rain occurs soon wilting;

d) deciduous type. Diseased upper leaves first symptoms, chlorosis, downward curl, wilt down, quickly shedding light pole in front diseased dead Serve, Leaf, bud, young bolls are off. Verticillium wilt diseased root, stem vascular bundles become brown, but light color than wilt. A.

2.2 Pathogen morphology Colonies on PAD medium white to gray, fluffy, black granular Microsclerotia produce. Conidiophores erect, septate, colorless To pale, (110µm ~ 130µm) × 2.5µm, each section whorls 3 to 4 small terrier (Verticillium) on the stem, may have a ~ 4, also the top student Small Terrier (top sticks). Small-scale Terrier (16µm ~ 35µm) × (1µm ~ 2.5µm), sporulation bottle small stem ends of the continuous production of conidia, Fugitive gathered into a ball-shaped head spores. Conidia colorless, single cellular, elliptical, cylindrical, size (2.5µm ~ 8µm) × (1.4µm ~ 3.2µm). Microsclerotia irregular spherical or elongated, dark brown. Size (50µm ~ 200µm) × (15µm ~ 50µm). A. 3 biological characteristics and transmission A.

3.1 Biological characteristics The optimal growth temperature Verticillium fungus is 20 °C ~ 25 °C, at 10 °C ~ 30 °C can grow at 33 °C not the vast majority of strains Growth, but there are high temperature strain can grow slowly. Soil moisture content of 20% in favor of the formation of micro-sclerotia, more than 40% negative effect on their shape to make. Microsclerotia resistant to 80 °C -30 °C high temperature and low temperature. Microsclerotia germination optimum temperature of 25 °C ~ 30 °C. A.

3.2 transmission Verticillium pathogen mainly micro-sclerotia overwinter in the soil, but also in cottonseed inside and outside, plant debris and contaminated the cotton seed hulls, cottonseed Bread and no decomposition of farmyard manure in winter. Contaminated seed is an important way distant of disease, in addition to contaminated soil, manure, cottonseed meal, cottonseed Housing, water and other farm machinery, and also pass the disease pathway.

Appendix B

(Informative) Cotton seed production prevention measures B.

1 Base Selection Base should be established in the region through strict investigation without Verticillium occurred. In the area of cotton verticillium wilt occurs, the base should have a large field of disease-free area. Surrounding fields with isolated disease-free conditions (away from traffic arteries And villages, not connected with the surrounding cotton fields, the terrain is higher), it can be found after the quarantine Upland Rotation. B. 2 seed sources and disinfection Base seed from disease-free areas, the "phytosanitary certificate", and without quarantine objects that pass the quarantine. Before sowing seed treatment use of effective implementation of the disinfection process. B. 3 prevention measures Base does not assume a variety of cotton varieties (lines) Regional Trial task. The base is really necessary to introduce new varieties and breeding material, it should be reported to the local plant quarantine department. The introduction of materials strict disinfection treatment, Under quarantine in the nursery environmental conditions conducive to the onset, try to grow more than 2 years, we found no quarantine in order to use at the base. Do not use without a cake and hot-pressed cotton scraps to field the base. Ward and prevent disease-free interval string irrigation water. Do not use the contaminated soil transplanting seedlings, seedling raising soil to go through dazomet disinfection. Prohibit the introduction of contaminated soil from the ward melons, vegetables and other seedlings. Base should use special tools to manage or hoe, special sun flower, special warehouse storage, ginning plane. B. 4 disease treatment Cotton Wilt found cottonseed without breeding. Tian strict blockade disease. prepare strain mark; removal and destruction of diseased plants and focus on plant debris, weeding host; timely disinfection disease point soil.

Appendix C

(Normative) Pest survey sampling record sheet Table C. 1 pest survey sampling record sheet Numbering. Production/operator address and zip code Contact/contact person in charge phone Survey Sampling Location Date sample Numbering Botanical name (Chinese name and scientific name) Variety name plant Growth Period Investigation on behalf of The number or area plant source Descriptions of the symptoms. Occurrence and Prevention and the reasons. Sampling methods, location and sampling ratio. Remarks. Sampling units (stamp). Preparer (Signed). year month day Production/operator Site person in charge year month day

Note. This unitary two together, the first joint sampling unit archive, the second cross-linking examined units.

Appendix D

(Normative) Pest Identification Report Sample Table D.

1 Pest Identification Report Sample Numbering. Plant Names cultivar name Plant growth of the number of samples Sampling site Source sample submission date inspection person Tel inspection unit Detection and identification methods. Detection and identification results. Remarks. Appraiser (Signed). Approved by (signature). Identification Seal. year month day

Note. This triplicate testing unit, examined units and quarantine agencies each.

Appendix E

(Informative) Isolation and Detection of Verticillium E. 1 medium 2% water agar plate medium. agar 20g, water 1000mL. PSA plate medium. potato 200g, sugar 15g, agar 20g, water 1000mL. Cottonseed meal agar medium. cottonseed cake flour 10g, 95% ethanol (alcohol) 17mL, streptomycin 40µg/mL, agar 7.5g, water 1000mL. E.

2 Isolation and Detection The discoloration of the vascular bundles of cotton stalks cut into small pieces 0.5cm long, running water 24h, or with 2% to 3% available chlorine hypochlorous acid Sodium liquid surface disinfection 1min ~ 2min, after sterile water, planted in 2% water agar plates, incubated at 22 °C ~ 25 °C 10d ~ 15d. Generating dissecting microscope to check whether the disease stalk segment ends Round branched conidiophores. If so, then transplanted to the medium level of PSA Board, continued to train. When generating Microsclerotia, compared with Verticillium full. It may be surface sterilized stem disease, planting beds in cottonseed meal agar On medium plate, cultured 10d ~ 15d, according to Verticillium and Microsclerotia formed, identified as Verticillium full. E. 3 moisturizing culture and detection Several small pieces of vascular discoloration taking cotton stalk, after clean with 70% ethanol (alcohol) soaked surface disinfection 3min, won 0.1% Mercuric chloride (corrosive sublimate 1g, concentrated hydrochloric acid 2.5mL, water 1000mL) disinfection 1min ~ 3min, and washed three times with sterile water. Fresh cotton can First in 95% ethanol (alcohol) in the prepreg after burning off the residue ethanol (alcohol) on the alcohol lamp flame, torn skin. Stem After disinfection treatment 3cm ~ 5cm long cut into small pieces and placed drops of water droplets on a sterile glass slide, each put three or four, into a double absorbent paper pad Petri dish, cover pan lid, at 22 °C ~ 22.5 °C or 27 °C ~ 30 °C incubator or room moisturizing culture 2d ~ 5d, white mycelium grows When picked drop a small amount of mycelium in sterile water droplets water droplets slide, gently stir the dispersion was observed under biological microscope, according to dendritic round spore Sub stem form, identified as Verticillium spores.

Appendix F

(Informative) Specimen preparation and preservation F. 1 dried specimens Quarantine pest specimen after drying, fill in the label, indicating the source of specimens (host, acquisition time, place, name of the variety, gatherers) And at the time, producers and the like. Specimen box corners repellents placed in a dry, cool and lockers for storage. F. 2 specimen liquid immersion Immediately after boiling water scalded to death by insect larvae into 70% ethanol (medical alcohol) soaking save.

Appendix G

(Informative) Cotton seed treatments G. 1 sulfuric acid from cashmere law G.

Appendix H

(Normative) Quarantine certificate Table H. 1 quarantine certificate Numbering. Plant variety name or product name Number Area Place of origin Production unit or head of household contacts Address Phone Quarantine Results. Inspectors (Signed). year month day Plant quarantine agencies validation opinion Phytosanitary Agency (quarantine seal) year month day NOTE. This permit is valid for 1 year and keep it, is not transferable, to be transported to the plant or product, by virtue of permits for the "phytosanitary and quarantine agencies to plant certificate". Quarantine certificate (stub) Numbering. Plant variety name or product name Number Area Place of origin Production unit or head of household contacts Address Phone Quarantine Results. Inspectors (Signed). year month day Plant quarantine agencies validation opinion Phytosanitary Agency (quarantine seal) year month day

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This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 24 pages — is available in the English PDF.

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Editions of GB 7411

Edition	Title	Revision	Status
GB 7411-2009	Quarantine protocol for cotton seeds in producing areas	current edition	Current
GB 7411-1987	Plant quarantine rules for producing areas of stock (superior) cotton seed	previous edition	Superseded

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