GB 2726-2016Hygienic standard for cooked meat products (English PDF)

3.1 Reagents

3.1.1 Sodium chloride (NaCl).

3.1.2 Anhydrous sodium sulfate (Na2SO4).

3.1.3 Ethyl acetate (C4H8O2). chromatographic pure.

3.1.4 Anhydrous ethanol (C2H5OH). chromatographic pure.

3.1.5 Water-based, acidic, alcohol food simulant. the reagents used are in

accordance with the provisions of GB 31604.1.

3.1.6 Sunflower seed oil. see Table B.1 in Annex B for specifications.

4.5 Volumetric flask. 50 mL, 100 mL, 1000 mL.

4.6 Organic filter. 0.45 µm.

4.7 Pipettes. 2 mL, 5 mL, 10 mL.

4.8 Plugged centrifuge tube. 10 mL.

4.9 Adjustable pipettes (1 µL ~ 20 µL, 20 µL ~ 100 µL, 100 µL ~ 5000 µL).

5.1 Sample migration test

In accordance with the requirements of GB 5009.156 and GB 31604.1, the

sample shall be subjected to a migration test to obtain a food simulant test

solution. If the food simulant test solution cannot be immediately used for the

further test, the food simulant test solution shall be preserved at 0°C ~ 4°C

refrigerator in the dark.

It shall cool or recover the obtained food simulant test solution to room

temperature before the further test is carried out.

5.2 Preparation of test solution

5.2.1 Preparation of water-based, acidic, alcohol simulant test solutions

Pipet 2.0 mL of the water-based, acidic, alcohol simulants obtained from the

migration test in 10 mL plugged centrifuge tube. Respectively add 0.5 g of

sodium chloride and 2 mL of ethyl acetate into the centrifuge tube. After

vortex extraction (1500 r/min) for 1.5 min, put it aside for 2 min. Remove the

supernatant. Use anhydrous sodium sulfate to dry. Filter it through a 0.45 µm

filter for gas chromatography-mass spectrometry.

5.2.2 Preparation of sunflower seed oil simulant test solution

Pipet 2 g (to the nearest 0.1 mg) of sunflower seed oil food simulant obtained

from the migration test into the 10 mL plugged centrifuge tube. Add 2 mL of

ethyl acetate into the centrifuge tube. After vortex extraction (1500 r/min) for 1

min, put it aside for 2 min. Centrifuge in the high-speed centrifugation (4000

r/min) for 5 min. Immediately remove the supernatant. Filter it through a 0.45

µm filter for gas chromatography-mass spectrometry.

5.3 Preparation of blank test solution

Process the food simulant not contact with food contact material according to

the steps of 5.2.1 and 5.2.2.

The blank solution and food simulant test solution shall be sequentially

injected. The blank area is deducted so as to obtain the peak area of methyl

methacrylate.

5009.156 Obtain the amount of methyl methacrylate in the food simulant and

products. The result is retained to two digits after the decimal point.