GB 19176-2010Sugar beet seed (English PDF)

Foreword

All the technical contents of this standard is mandatory. The standard reference to "international inspection of seed" 1996 edition, revised in accordance with relevant provisions of the "People's Republic of China Seed Law" and substitute GB 19176-2003 "sugar beet seed." This standard compared with GB 19176-2003, the main changes are as follows:

--- Amendments to the normative references;

--- Revised terms and definitions;

--- Modified seed category and some technical indicators;

--- Increasing the particle size measurement method;

--- Modify the packaging, labeling, transportation and storage requirements;

--- Amendments to Appendix A (normative) Clarity analysis;

--- Revised Appendix B (normative) germination test;

--- Revised Annex C (normative) triploid rate test. The Standard Appendix A, Appendix B, Appendix C are normative appendix. The standard proposed by the People's Republic of China Ministry of Agriculture. This standard by the National Standardization Technical Committee crop seeds. This standard was drafted. the Ministry of Agriculture Quality Supervision and Testing Center beets, beet Institute of Chinese Academy of Agricultural Sciences, the National Agricultural Technology Extension and Service Center, Xinjiang Yili Seed Management Station (Xinjiang Yili Crop Seed Quality Supervision and Testing Center), Heilongjiang Province, sweet Seed Management Station, Baotou Huazishiye Co., Ltd., Heilongjiang University. The main drafters of this standard. Wu Yumei, Chen Lianjiang, Wu Qingfeng, Bai Teng Qian Lv Xiaogang, Xia province, Qin tree only. This standard replaces the standards previously issued as follows:

--- GB 19176-2003. Sugar beet seeds

1 Scope

GB 19176-2010 is the Chinese national standard on sugar beet seed, in the field of agriculture. It carries no /T suffix, which in the Chinese system means compliance is mandatory: a product or a practice within its scope has to meet it to be lawfully made, sold or carried out in China. It was issued on 14 January 2011 by the General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China; Standardization Administration of China, and has been in force since 1 January 2012. Classification: ICS 65.020.20, CCS B61. This page is published from the official record of the standard held by the Chinese standards administration: the identification, the dates, the classification and the issuing body are taken from there. The clause text, the tables and the numeric limits are in the document itself, which is delivered complete in English translation.

This standard specifies the terms and definitions sugar beet (Betavulgarisvar.saccharifera) seeds, quality requirements, test side Method, inspection rules, packaging, labeling, transportation and storage requirements. This standard applies to the territory of People's Republic of China the production and sale of sugar beet seeds.

2 Normative references

The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard.

GB/T 3543.2 agricultural seed testing protocols for sampling

GB/T 3543.3 crop seed testing Clarity Analysis Rules for the inspection

GB/T 3543.6 crop seed moisture determination

GB/T 3543.7 crop seed testing other items test

GB 20464 General labeling of agricultural seeds

3 Terms and Definitions

The following terms and definitions apply to this standard.

3.1 For sugar beet seed production of raw materials.

3.2 Breeder seed breederseed Breeders bred stable hereditary characteristics consistent pedigree or seed strains.

3.3 Stock basicseed Breeder seed multiplication with the first generation to the third generation, seeds and confirmed to meet the specified quality requirements.

3.4 Diploid diploid In beet somatic cells contain two genomes (2x = 18 chromosomes).

3.5 Triploid triploid In beet somatic cells, containing three genomes (3x = 27 chromosomes).

3.6 Tetraploid tetraploid In beet somatic cells genome contains four (4x = 36 chromosomes).

3.7 Common polyploid exoploid Diploid and tetraploid strains mutual parental strain, according to a certain proportion of the natural seed of hybrid obtained.

3.8 Male polyploid polyploidbasedonCMS In male sterile diploid (or tetraploid) lines as female, with the pollen of tetraploid normal (diploid) of the parent strain, according to a certain ratio Example formulated natural hybrid hybrids.

3.9 Beet CMS beetmalesterile Because beet pollen mother cells degenerate without having the ability to pollinate, also known as sugar beet male infertility.

3.10 Single germ geneticmonogermseed Embryo containing only one seed, also known as single species of grain, fruit species, intraspecific ball single bud obtained by genetic.

3.11 Multi-germ multigermseed Intraspecific ball with more than two (including two) types of seed embryos, also known as multi-grain species, complex fruit species, multi-bud seed.

3.12 Polished species polishedseed Simply processed to remove the bulb surface calyx, in addition to the genetic nature, other characteristics (such as particle size, specific gravity, etc.) there are significant changes in the sugar beet seed.

3.13 Pelleted seeds peletedseed In order to meet a single seed precision sowing the seeds of beets made no significant difference in size and shape similar to spherical. Pelleted seeds In addition to adding sub pelleting substances, it may contain pesticides, fungicides, dyes or other additives.

4 Quality requirements

4.1 polyembryonic sugar beet seeds Polyembryonic sugar beet seeds shall meet the minimum requirements of Table 1. Table 1 sugar beet seed quality requirements polyembryonic Seeds category Germination rate/% not lower than Cleaness/% not lower than Triploid rate /% not lower than Moisture /% not higher than Particle size/mm Diploid Stock 80 98.0 - 14.0 >=

6 Inspection rules

6.1 for sampling OK for sampling methods and seed lot shall implement the provisions of GB/T 3543.2 of.

6.2 Quality judgment rules Quality judgment rules shall implement the provisions of GB 20464. 7 packaging, labeling, transportation and storage

7.1 Packaging Sugar beet seeds can be used plastic bags, cardboard boxes, paper bags, woven bags, sacks and other packaging or packaging. Pelleted seeds in one unit Smallest packaging unit, each unit 100 000. Graded particle size in the predetermined range, the total mass of the mass percentage of the total of the parts And shall not be less than 98.5%. Other types of sugar beet seeds, each smallest packaging unit, provisions shall be not less than the diameter of the seed 70.0%.

7.2 mark Bags and cartons (including each box individually wrapped) beet seed sales should be attached with the label. The label shall comply with GB 20464 Provisions.

7.3 Transport Prohibit harmful, toxic or other polluting materials mixed storage, mixed operation, to prevent moisture. Transport vehicles should have tarpaulin Gaiyan ship Transport should have under the cushion.

7.4 Storage Beet seed storage place to have rain, moisture, fire prevention, ventilation and other conditions, the warehouse has subsidiaries Shaichang prohibited and flammable, explosive Goods and supplies of fertilizers, pesticides and other common storage. Beet seed stack seed storage should be convenient for sampling. Seed should pass before the library of the inspection, unqualified Seeds are not allowed out of the library.

Appendix A

(Normative) Clarity Analysis A.1 apparatus and appliances Decimator; different aperture sieve set (including oscillator); balance. a sense of the amount of 0.1g, 0.01g and 0.001g. A.2 Determination Program Using a sample analysis. Check A.2.1 of heavy impurities Weigh at least (single germ 125.0g) (M) to send test samples 250.0g, the pick and sugar beet seed size or quality significantly different And seriously affect the outcome of impurities, such as earth, small stones or other large seeds and other weighing (m), and then the heavy impurities (m) separated into its He seeds (m1) and impurities (m2). A.2.2 dispensing test sample Clarity test samples analyzed (a) should have been singled out for examination of samples of heavy impurities dispensation (according to GB/T 3543.2 in laboratory The method of dispensing test sample) about 50.000g, weighed. A.2.3 sample separation The test sample is placed in a predetermined aperture test sieve sieve treatment. Seeds Filter sifting 2min (pelleted seeds 1min); hand-sifting methods Reciprocating 20 times (10 times back and forth seed coat), converted to 90 °, and then back and forth 20 times (10 times back and forth again pelleted seeds), after the end of the beat. However After the sieve net seed (sugar beet bulbs and broken bulbs) of impurities such as small stones, clods, mouse bird droppings, beet plant stems and leaves and small off Flowers, debris and other non-beet seeds and other plant seeds picked, separated; but also other plant seeds under the sieve pick from impurities separated. Finally, the impurities on the screen and the screen, all other plant seeds are combined, so separates the sample into pure seeds, other plant species Child three components and impurities. Then three components were weighed (accurate to 0.001g), in grams (g) that the conversion percentage becomes. Standard test sieve specifications. round hole sieve sieve sieve and long holes are high 50mm, diameter 200mm. Hole sieve hole diameter greater than or equal 2.0mm, 0.25mm each to a differential; long hole sieve hole length 20mm, large than or equal to 2.0mm, with 0.5mm as a differential; Inspection germ with round hole screen, multi-germ test with a long hole sieve. A.3 Results Calculation and representation Execution according to the provisions GB/T 3543.3 of.

Appendix B

(Normative) Germination Test Using paper cassette paper between folds law. B.1 instruments, appliances and reagents Number of tablets; germination box (size.200mm × 110mm × 75mm); sprouting wrinkled paper (mass per square meter of 70g ~ 90g, suck Water was 220% ~ 250%); germination boxes or germination chamber; sodium hypochlorite; thiram like. B.2 Test Procedure B.2.1 Sampling After thorough mixing of the net from the seeds by hand or number of tablets to take 400 random number seed. We should be careful not to pick the seeds in order to avoid Free bias the results. Usually 100 to a repeat, repeated four times. B.2.2 Rinse seeds Seed samples will be available for inspection on the mesh bag, with 20 °C ~ 25 °C water rinse (more germ 2h, single germ 4h). B.2.3 seed disinfection Rinse the good seeds into the 0.3% to 0.5% aqueous solution of thiram seed soaking 10min. B.2.4 dried seeds Seeds were air-dried at room temperature and ventilation. Multi-germ dried 10min ~ 30min, a single germ dried 1h. B.2.5 germination box disinfection Germination boxes placed before using a 0.1% aqueous sodium hypochlorite solution to soak 3min ~ 5min, washed with water and then dried. B.2.6 boxing method First coated paper laid on the bottom of the germination box, and then folds the paper expand on the germination cover paper. Then quantitative sprayer 32mL ~ 34mL (single germ 30mL ~ 32mL) distilled water sprayed evenly germinate paper. Finally put two seeds in each of the folds of the pitch to all Even between adjacent folds seed position to be staggered, each box 100 seeds. Folding cover cover paper, reclosable lid, put a plastic bag, placed in germination boxes Germination rack (chamber) inside. B.2.7 germination temperature Germination using a constant temperature, germination boxes (room) temperature during germination germination should be as uniform as possible. Predetermined temperature at 23 °C ~ 25 °C. B.2.8 germination Light There are carried out in the light conditions, light intensity 1000lx ~ 1500lx, illumination time of 8h. B.2.9 Test duration Test duration 10d. Initial counting time 4d, the last time the count was 10d. The number of normal seedlings are calculated. B.2.10 Seedling Identification Each plant seedlings must be prescribed standards (B.2.10.1 ~ B.2.10.2) were identified. To identify the main structure of seedling development has Proceeds to a certain period. In the counting process, well-developed seedlings should sprout normal folds in the paper are detected, the suspected or damage, abnormal Shaped or uneven seedling, usually column to the last count. Or moldy seeds should germinate seedlings badly decomposed paper wrinkles removed, and at any time count. B.2.10.1 normal seedlings Complete seedlings, seedlings with minor defects or secondary infections are normal seedlings. Identification of normal seedlings specific criteria are as follows: B.2.10.1.1 complete Seedlings The main structure seedlings grew well, a complete, uniform and healthy.

a) an elongated primary roots, usually covered with hairs, the end of the fine tip;

b) has an upright, slender and a hypocotyl elongation capacity;

c) having two expansion was leafy green cotyledons. B.2.10.1.2 seedlings with minor defects The main structure of the seedlings appear some minor flaws, but in other aspects of a balanced growth, and the same test in quite the full seedlings. B.2.10.1.3 secondary infection seedlings Caused by a fungal or bacterial infection, so that the main structure of morbidity and seedling rot, but there is evidence that the source of the disease does not come from the seeds themselves. B.2.10.2 abnormal seedlings The entire seedling deformity; fracture; cotyledons than root to grow; two seedlings together; yellowing or whitening; thin; edema-like; the primary root sense Dye caused by decay. B.3 retests When the test in the following cases should be re-tested. B.3.1 When they find the test conditions, when the seedlings have identification or counting errors, the same method should be used to re-test. B.3.2 When germination test gap between repeated four times exceeds the maximum allowable gap Table B.1, the same method should be used to re-test Experience. If the re-test results consistent with the first, the gap does not exceed the maximum allowable gap Table B.2, two tests will be flat Mean reporting on the results of a single; if re-test and the first results are not consistent, the gap exceeds the maximum allowable gap B.2 table shown, The same method is used for the third test, the results of two tests until a consistent date and the average of the two tests to fill in Results list. B.3.3 In case of power failure, germination boxes (chamber) can not maintain the conditions necessary for seed germination requirements, the batch of samples should be re-measured. Table maximum allowable gap the same germination test was repeated four times between B.1 (2.5% significant level of two measured)% The average germination rate 50% to 50% of The maximum allowable gap 93 to 94 91 to 92 89 to 90 87 to 88 84 to 86 81 to 83 78 to 80 73 to 77 67 to 72 56 to 66 51 to 55 7-8 9-10 11 to 12 13 to 14 15 to 17 18 to 20 21 to 23 24 to 28 29 to 34 35 to 45 46 to

50 Table B.2 of the same or different from the laboratory germination tests allow the gap between the same or different samples for examination (2.5% significant level of two measured)% The average germination rate 50% to 50% of The maximum allowable gap 98 to 99 95 to 97 91 to 94 85 ~ 90 77 to 84 60-76 51 to 59 2-3 4-6 7-10 11 to 16 17 to 24 25 to 41 42-50 B.4 Calculation and Expression Test results are expressed as a percentage of grains. When four replicates a percentage of normal seedlings were tested within the maximum allowable gap (See Table B.1), it is represented by its average germination percentage. Normal seedlings, abnormal seedlings and non-germinated seeds percentage of the sum must be 100, the average percentage rounded to the nearest integer. Completing the germination results, if any of them once the result is zero, then the symbol "-0 - "Fill in the grid. Germination rate = All normal seedlings sprouting bulbs final number Planting for ball number × 100% (B.1) B.5 tolerance Allow the same gap between the germination test was repeated four times to perform in accordance with Table B.1; the same or different from the same or different laboratory samples for examination Allowable gap between germination test performed in table B.2; germination testing in accordance with the allowable gap in the sampling table, uniform inspection, arbitration inspection, periodic inspection B.3 perform predetermined value refers to the quality standards, contracts, and other technical specifications of the label. Table B.3 germination test compared with a predetermined value allowable gap (5% significance level of a measurement)% Standard germination rate 50% to 50% of Allowable gap 96 to 98 92-95 87 to 91 80 to 86 71 to 79 58 to 70 51 to 57 3-5 6-9 10 to 14 15 to 21 22 to 30 31 to 43 44 to 50

Appendix C

(Normative) Triploid rate test Acetic acid lichen red staining, young radicle tip number of chromosomes in the nucleus test to determine the triploid rate. C.1 and reagent preparation Kano fixative. ethanol acetate A three ice mix. Softeners. A concentrated hydrochloric acid plus a mix of 95% ethanol. 2% acetic acid lichen red solution. Weigh 2.0g lichen red reagent, 45% glacial acetic acid dissolved in 100mL of solution. 45% glacial acetic acid. Pretreatment solution. 8-hydroxy quinoline or p-dichlorobenzene and the like. C.2 apparatus and appliances Germination boxes; biological microscope; slides; cover sheet; weighing bottles. C.3 inspection procedures C.3.1 germination and coverage Take about 100 test samples were germinated in young radicle tip cell division peak period (usually 2d ~ 3d after germination), select White Color, stout, length 8mm ~ 15mm 3mm ~ 5mm of root tip portion. C.3.2 pretreatment Will be taken into the root tip containing 8-quinolinol or a saturated solution of p-dichlorobenzene weighing bottle, capped, at room temperature 20 °C ± 2 °C Under treatment 2h. Or apical load weighing bottle filled with distilled water, placed in the refrigerator at 2 °C ~ 3 °C condition pretreatment 24h, the cells Split stay in mitotic metaphase chromosomes shorten make thicker, easy to observe. C.3.3 fixed and isolated (softened) The pretreated apical rinsed with distilled water twice to three times after discharge Ruka Nuo fixative 2h ~ 3h; then the material after fixation Remove, rinse with distilled water into the softener, the process was transparent to the root tip is moderate (about 5min) at room temperature. C.3.4 stained with producer The isolated good material washed with distilled water twice or three times, cut the apical 2mm ~ 3mm on a slide, dropping 2% acetic acid Lichen red staining solution 5min ~ 15min. 45% acetic acid and then drop to the dichroic 1min ~ 5min. Stamped sheet, above the filter mat After the paper gently knock pressure, so that the weight of thin square root tip is appropriate. C.3.5 Test Looking first at low magnification position of dividing cells, then transferred to high-powered microscope, identified the number of chromosomes split phase, three times determined body. A test sample to be effective examination of 50 tablets each check out the number of chromosomes split effective after two phases is determined. C.4 Results Calculation and representation Test results expressed as a percentage of triploid number of pieces of sheet 50 effectively. The results rounded to the nearest integer. P = T50 × 100% (C.1) Where. P

--- triploid rate; T

--- triploid number of pieces, pieces;

--- Effective amount specified sheet 50, sheet.