GB/T 9695.31-2008Meat products - Determination of total sugars content (English PDF)

Foreword

GB/T 9695 consists of the following components.

--- GB/T 9695.1 "Meat and meat products - Determination of free fat";

--- GB/T 9695.2 "Meat and meat products - Determination of fatty acid";

--- GB/T 9695.3 "Meat and meat products - Determination of iron content";

--- GB/T 9695.4 "Meat and meat products - Determination of total phosphorus content";

--- GB/T 9695.5 "Meat and meat pH measurement";

--- GB/T 9695.6 "meat Carmine colorant determination";

--- GB/T 9695.7 "Meat and meat products - Determination of total fat content";

--- GB/T 9695.8 "Meat and meat products - Determination of chloride content";

--- GB/T 9695.9 "Meat and meat products polyphosphate determination";

--- GB/T 9695.10 "meat and meat products BHC and DDT residues determination";

--- GB/T 9695.11 "Meat and meat products - Determination of nitrogen content";

--- GB/T 9695.13 "Meat and meat products - Determination of calcium content";

--- GB/T 9695.14 "meat starch content determination";

--- GB/T 9695.15 "Meat and meat products - Determination of moisture content";

--- GB/T 9695.17 "Meat and meat products - Determination of glucono-lactone -delta-content";

--- GB/T 9695.18 "Meat and meat products - Determination of ash";

--- GB/T 9695.19 "Meat and meat sampling method";

--- GB/T 9695.20 "Meat and meat products - Determination of zinc";

--- GB/T 9695.21 "Meat and meat products - Determination of magnesium content";

--- GB/T 9695.22 "Meat and meat products - Determination of copper content";

--- GB/T 9695.23 "Meat and meat products - Determination of hydroxyproline content";

--- GB/T 9695.24 "Meat and meat cholesterol content determination";

--- GB/T 9695.25 "Meat and meat products - Determination of vitamin PP";

--- GB/T 9695.26 "Meat and meat products - Determination of vitamin A content";

--- GB/T 9695.27 "Meat and meat products - Determination of vitamin B1";

--- GB/T 9695.28 "Meat and meat products - Determination of vitamin B2";

--- GB/T 9695.29 "Meat vitamin C content determination";

--- GB/T 9695.30 "Meat and meat products - Determination of vitamin E content";

--- GB/T 9695.31 "Meat total sugar content determination."

This section GB/T 9695 of Part 31.

This Part replaces GB/T 9695.31-1991 "Determination of total sugar content of meat."

This section compared with the GB/T 9695.31-1991 The main changes are as follows.

--- According to GB/T 1.1-2000 "Standardization Guide Part 1: Standard structure and writing rules" and GB/T 20001.4-2001 "Rules for drafting standards - Part 4: Chemical analysis method" has been restructured and text modify;

--- According to GB/T 1.1-2000, delete the English name of "Methodfor";

--- Chapter 1 increased the scope of application of the second law of explanation;

--- Chapter 2 increase Normative references GB/T 6682-2008;

--- Section 3 is more accurately expressed in the "absorbance value is proportional to the concentration of sugar in the same" pre-added "within a certain range";

--- Chapter 4, "the reagents were of analytical grade, water is distilled water or equivalent purity" to "unless otherwise specified, the test Agents were of analytical grade, "added the method of preparation portion of the solution, increasing the clause" 4.1 Water. should be consistent with GB/T 6682-2008 three water requirements ";

--- Chapter 8 changed the formula of expression;

--- With "9 Precision" and its contents instead of "9 to allow poor" and its contents;

--- Added "Test Report" chapter;

--- Added a second Law "direct titration."

This part of the National Meat egg products Standardization Technical Committee.

This section is drafted. Shenzhen Academy of Metrology and Quality Inspection, China Chamber of Commerce Business Standard Centre.

1 Scope

GB/T 9695 provisions of this part of the determination of the total sugar content of meat products.

This part of the first method for the determination of total sugar content of meat products; second method is suitable for meat products contain no starch, total sugar content Determination.

2 Normative references

The following documents contain provisions which, through reference to GB/T 9695 to the present, constitute provisions of this section. For dated reference documents Member, all subsequent amendments (not including errata content) or revisions do not apply to this section, however, encouraged to reach under this section Parties to research agreement to use the latest versions of these documents. For undated reference documents, the latest versions apply to this section.

GB/T 6682-2008 analytical laboratory use specifications and test methods (ISO 3696. 1987, MOD)

GB/T 9695.19 Meat and meat products - Sampling Methods

3 Principle

The first method spectrophotometry.

After a sample of sugar by hot water extraction, dehydration with sulfuric acid, furfural or furfural derivatives. Phenolic compound with an aromatic condensation product A yellow substance at a maximum absorption at 470nm, within a certain range absorbance value is proportional with the concentration of sugar, as measured sugar content.

4 Reagents

Unless otherwise specified, the reagents were of analytical grade.

4.1 Water. should be consistent with GB/T 6682-2008 in three water requirements.

4.2 phenol solution. Weigh 5g phenol dissolved in 100mL of water. Dark storage.

4.3 of concentrated sulfuric acid (rho20~1.84g/mL).

4.4 Glucose standard solution. Accurately weigh 1.000g dried 2h of 96 °C ± 2 °C of pure glucose, dissolved in water was added 5mL Hydrochloric acid, and water volume to 1000mL. This is equivalent to 1.0mg per milliliter solution of glucose.

4.5 amylase solution. Weigh 0.5g amylase dissolved in 100mL of water.

4.6 iodine - potassium iodide solution. Weigh potassium iodide 3.6g, 1.3g of iodine dissolved in water and diluted to 100mL.

5 Apparatus

5.1 Conventional laboratory instruments.

5.2 meat grinder. pore size of not more than 4mm.

5.3 Spectrophotometer.

6 Sample

6.1 according to GB/T 9695.19 sampled.

6.2 Take a representative sample of not less than 200g, twice with a meat cutter and mix.