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GB/T 8314-2013Tea - Determination of free amino acids content (English PDF)

茶 游离氨基酸总量的测定

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Issued by

SAMR; SAC

Level / Type

National · Recommended

Issue date

December 31, 2013

Implementation date

June 22, 2014

Scope

GB/T 8314-2013 is the English-translated version of 茶 游离氨基酸总量的测定.

GB/T 8314-2013 is the Chinese standard "Tea - Determination of free amino acids content". Its scope clause reads: This standard specifies the apparatus and appliances, reagents and solutions, operating methods and results Determination of free amino acids in tea calculated method.

This standard applies to the determination of total free amino acid in tea. Its clauses include terms and definitions; reagents and solutions.

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Document preview — GB/T 8314-2013

National Standard of the People's Republic of China

ICS
67.140.10
Classification
X 04
Replacing
GB/T 8314-2002

Issued by: State Administration for Market Regulation; Standardization Administration of the PRC

Contents

  • 1 Scope
  • 2 Normative references
  • 3 Terms and Definitions
  • 6 Reagents and solutions

1 Scope

This standard specifies the apparatus and appliances, reagents and solutions, operating methods and results Determination of free amino acids in tea calculated method.

This standard applies to the determination of total free amino acid in tea.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein

Member. For undated references, the latest edition (including any amendments) applies to this document.

GB/T 8302 sampling tea

Sample Preparation and Determination of dry matter content GB/T 8303 Tea grated

GB/T 8312 Determination of caffeine in tea

3 Terms and Definitions

The following terms and definitions apply to this document.

3.1 Free amino acids

Tea flooding alpha- amino acids having a free state in the physical form.

Principle 4

alpha- amino acid at pH8.0 conditions were hot with ninhydrin to form a purple complex measured by spectrophotometry at a specific wavelength

Its content.

5 apparatus and appliances

5.1 Analysis of balance

a sense of the amount of 0.001g.

5.2

Spectrophotometer.

5.3 colorimetric tube

a plug, 25mL.

6 Reagents and solutions

6.1

Unless otherwise indicated, the methods used were of analytical grade reagents (AR), distilled water.

6.2 pH8.0 phosphate buffer

6.2.1 1/15mol/L disodium hydrogen phosphate

Weigh 23.9g twelve - hydrated disodium hydrogen phosphate (Na2HPO4 · 12H2O), add water to dissolve into

1L volumetric flask, dilute to the mark, shake.

......
This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 15 pages — is available in the English PDF.

Referenced standards

Similar standards

Editions of GB/T 8314

EditionTitleRevisionStatus
GB/T 8314-2013Tea - Determination of free amino acids contentcurrent editionCurrent
GB/T 8314-2002Tea - Determination of free amino acids contentprevious editionSuperseded

This page sells the current edition, GB/T 8314-2013. Earlier editions are listed for reference only.

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