GB/T 35811-2018Method for analysis of forestry biomass—Determination of starch content (English PDF)

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009.

1 Scope

This standard specifies the terms and definitions, principles, reagents, solution preparation, equipment, and operation steps for starch determination in forestry biomass raw materials. Steps, result processing and presentation, repeatability.

This standard applies to forest products (such as wood, bamboo, rattan, etc.), forestry residues (such as branches, sawdust, sawdust, shoots, slats, and cuts).

The determination of starch in forestry biomass raw materials such as harvesting residues and processing residues such as heads, shells, and kernels.

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this document. For undated references, the latest version (including all amendments) applies to this document.

GB/T 5009.3-2010 National Food Safety Standard Determination of Moisture in Food

GB/T 6682-2008 Analytical laboratory water specifications and test methods

GB/T 30366-2013 biomass terminology

3 Terms and Definitions

The terms and definitions defined in GB/T 30366-2013 and the following apply to this document. For ease of use, the following is repeated Some terms and definitions in GB/T 30366-2013.

4 Principle

Dry-based starch in forestry biomass is hydrolyzed into small molecule sugars by the action of alpha-amylase, and hydrolyzed by hydrochloric acid into single-reduced ones.

Sugar, which can be converted to starch content by reducing sugar content, can be used for quantitative analysis of starch.

5 Reagents

5.1 All reagents were of analytical grade except those specified for the reagents.

5.2 Iodine (I2).

5.3 Potassium iodide (KI).

5.4 Alpha-amylase. The activity unit is 3000~5000.

5.5 Anhydrous ethanol (C2H5OH).