GB/T 23197-2022Diagnostic techniques for avian infectious bronchitis (English PDF)

Foreword

This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for Standardization Work Part 1: Structure and Drafting Rules for Standardization Documents" drafting.

This document replaces GB/T 23197-2008 "Diagnostic Techniques for Chicken Infectious Bronchitis". Compared with GB/T 23197-2008, except In addition to structural adjustments and editorial changes, the main technical changes are as follows.

---Changed "clinical diagnosis" (see Chapter 5, Chapter 2 of the 2008 edition);

--- Added "sample collection" and "sample processing" (see 6.3 and 6.5);

---Changed the "virus isolation and identification" (see Chapter 7, Chapter 3 of the 2008 edition);

---Changed the "viral RT-PCR" and updated the detection primers (see 8.3, Chapter 4 of the 2008 edition);

--- Added "viral real-time fluorescent RT-PCR" (see Chapter 9);

--- Increased "virus genotype identification" (see Chapter 10);

---Changed the "blood coagulation and hemagglutination inhibition test" (see Chapter 11, Chapter 5 of the 2008 edition);

---Changed the "tracheal ring tissue culture serum neutralization test" (see Chapter 12, Chapter 6 of the 2008 edition).

Introduction

IB is an acute, highly contagious infectious disease of chickens. IB can occur in chickens of various ages, chickens less than 5 weeks old Especially susceptible, and the symptoms are more obvious. The clinical features of IB are mainly manifested as dyspnea, rales, coughing and sneezing; decreased egg production, egg quality declines; chicks appear to die. IB is widely prevalent all over the world and seriously endangers the production of chicken industry.

IBV belongs to Coronaviridae, Coronaviridae, and Gammavirus in classification, and is a single-stranded positive-sense RNA virus whose RNA is easily In terms of variation, IBV has diversity. Most IBV strains can cause specific lesions in the trachea, but some strains can cause kidney lesions and Genital tract lesions. At present, the IBV strains that have been isolated at home and abroad belong to more than 30 serotypes, and the cross-immunity between IBV strains of each serotype Low protection.

The diagnosis of IB is mainly carried out by etiological and serological tests. Due to the widespread use of IBV live strain vaccines in production to prevent this disease, this is Clinically, it increases the difficulty of diagnosing IB.

This document standardizes the clinical diagnosis technology of IB; standardizes the pathogenic diagnosis technology of IB, including virus isolation and identification, virus RT-PCR, virus real-time fluorescent RT-PCR, virus genotype identification; standardized IB serological diagnostic techniques, including hemagglutination and hemagglutination inhibition tests Test, tracheal ring tissue culture serum neutralization test. These diagnostic techniques are also recommended in the OIE Manual of Terrestrial Animal Diagnostic Tests and Vaccines.

1 Scope

This document specifies the clinical diagnosis, virus isolation and identification, virus RT-PCR, and virus real-time fluorescence of chicken infectious bronchitis (IB). Technical requirements for RT-PCR, virus genotype identification, hemagglutination and hemagglutination inhibition test, tracheal ring tissue culture serum neutralization test.

This document is applicable to the diagnosis, quarantine, surveillance and epidemiological investigation of IB.

2 Normative references

The content in the following documents constitutes the essential provisions of this document through normative references in the text, among which, the dated references For documents, only the version corresponding to the date is applicable to this document; for undated reference documents, the latest version (including all amendments) is applicable to this document.

GB 19489 General Requirements for Laboratory Biosafety

3 Terms and Definitions

The following terms and definitions apply to this document.

4 Abbreviations

The following abbreviations apply to this document.