GB 20287-2006Microbial inoculants in agriculture (English PDF)

3.1 Microbial inoculants in agriculture

The live bacteria preparations, which are processed from target microorganisms (effective bacteria), through industrialized production and propagation.

5.1 Bacteria species

The microbial strains, which are used in production, shall be safe and effective.

The producer shall provide the classification and identification report of the bacterial species, including complete information such as the scientific name of the genera and species, morphology,

5.2 Product appearance (sensory)

Powder products shall be loose. Granular products shall have no obvious mechanical impurities, be uniform in size, have water absorption.

5.3 Product technical index

5.3.1 The technical index of agricultural microbial inoculants products is as shown in Table 1, wherein the technical index of organic material decomposing agents are implemented in accordance with Table 2.

5.3.2 See Table 3 for harmlessness index of the microbial inoculants in agriculture.

6.1 Instrument and equipment

6.1.1 Biological microscope

6.1.2 Constant temperature incubator

6.1.3 Constant temperature drying oven

6.1.4 Ultra-clean workbench or clean room

6.1.5 Electronic balance (or precision balance)

6.1.6 Shaker

6.1.7 Steam sterilization pot

6.1.8 Test sieve

6.1.9 Acidity meter

6.2 Reagents

6.3 Testing of product parameters

6.3.1 Determination of appearance (sensory)

Take a small amount of samples. Place them in a white enamel tray (or white plastic palette). Carefully observe the color, shape, texture of the sample.

6.3.2 Determination of effective viable bacteria count

Use the plate counting method. Select an appropriate medium, according to the type of microorganisms tested.

If the dilution method [Most Probable Number (MPN) 5 pipe method] is used, it is performed in accordance with the provisions of Appendix C.

6.3.2.4 Colony count

Take the dilution plate, which appears 20 ~ 300 colonies, as the counting criteria (10 ~ 150 colonies for filamentous fungi). Respectively count the number of effective viable bacteria and the number of miscellaneous bacteria. When there is only one dilution AND the average number of colonies is between 20 ~ 300, the average number of colonies is used for calculation.

6.3.3 Determination of the number of fungi

Use Martin medium. The determination method is the same as 6.3.2.

6.3.4 Determination of the rate of miscellaneous bacteria

Except for the effective bacteria in the sample, all other bacteria are miscellaneous bacteria. The rate of miscellaneous bacteria in the sample is calculated according to formula (3).

6.3.7.3 Particle samples

The sample is first crushed and pass through a 1.0 mm test sieve. Make measurement, according to the method of 6.3.7.2.

6.3.8 Determination of the number of fecal coliforms

It shall meet the requirements of GB/T 19524.1.

6.3.9 Determination of the mortality of ascaris eggs

It shall meet the requirements of GB/T 19524.2.

6.3.10 Determination of cellulase activity and protease activity

It shall meet the requirements of Appendix D.

6.3.11 Determination of arsenic, cadmium, lead, chromium, mercury

It shall meet the requirements of 5.12 ~ 5.17 in GB 18877-2002.

6.3.12 Inspection of shelf life

Before the shelf life indicated in the product manual, measure the corresponding indicators of the product, according to the methods 6.3.1 ~ 6.3.11.

7.1 Sampling

The products, which are processed from each fermenter's bacteria liquid (or each batch of solid fermentation), as a batch, to carry out sampling inspection.

During the sampling process, it shall strictly avoid the contamination of bacteria.

7.1.1 Sampling tools

Sterile plastic bags (bottles), metal spoons, samplers, measuring cylinders, kraft paper bags, glue, sampling seals, sampling sheets, etc.

7.1.2 Sampling method and quantity

Generally, the sampling is carried out in the finished product warehouse, through the random method.

7.2 Inspection classification

7.2.1 Exit-factory inspection

7.2.1.2 The exit-factory inspection items are the items specified in 5.2 and 5.3.1, but do not include the shelf life.

7.2.2 Type inspection

7.3 Judgment rules

7.3.1 Any products, which meet the requirements of one of the following clauses, are qualified products

7.3.2 Any products, which meet the requirements of one of the following clauses, are unqualified products

8.1 Packaging

Choose appropriate packaging materials, containers, forms, methods, according to different product dosage forms, to meet the basic requirements of product packaging.

8.2 Markings

The content, which are marked on the label, shall comply with the provisions of national laws and regulations.

8.2.1 Product name and trademark

It shall indicate the general name of the product, that has been specified by the national standards and industry standards. For the name of the product OR the name of the product with a special purpose, it may be marked, under the general name of the product, by the font of smaller size.

8.2.2 Product specifications

It shall indicate the net weight of the product in each package; use the national legal unit of measurement. The error range of the marked net weight shall not exceed ±5% of its stated value.

8.2.3 Implemented standard of product

It shall indicate the standard number implemented by the product.

8.2.4 Registration certificate number of product

It shall indicate the valid registration certificate number of product.

8.2.5 Name and address of manufacturer

It shall indicate the name, address, postal code, contact number of the manufacturer, who has been registered in accordance with the law AND can bear the responsibility for product quality. For the imported products, it may be exempted from the marking of the name and address of the manufacturer;

8.2.6 Date of production or production batch number

It shall indicate the production date or production batch number of the product, on the production certificate or product packaging.

8.2.7 Shelf life

Use "shelf life _____ months (OR several days, years)" for presentation.

8.3 Transportation

There shall be covers, during transportation, to prevent exposure to rain, sun, high temperature. Take appropriate measures, when the temperature is below 0 °C, to ensure product quality. Handle with care, to avoid package damage. It is strictly forbidden to mix and transport it with other items, which are toxic and harmful to the microbial inoculants.

8.4 Storage

The products shall be stored in a cool, dry and ventilated warehouse. It shall not be stacked in the open air, to prevent the exposure to sun and rain; avoid the influence of adverse conditions. […]