GB/T 13917.7-2026Laboratory efficacy test methods and criteria for public health insecticides for pesticide registration - Part 7: Baits (English PDF)

4.2 Test Target

4.2.1 Flies Houseflies (Musca domestica) were reared according to the method in NY/T 1964.1 for sensitive strains. Test insects were selected on days 3-4 after emergence. Adult insects weighing (20.0±2.0) mg/female and (16.0±1.6) mg/male, with half females and half males.

4.2.2 Cockroaches German cockroaches (Blattella germanica) were reared according to the method in NY/T 1964.4, and 10-day-old test insects were selected. Adult insects aged 20 days, weighing (100.0±10.0) mg/female (non-podded) and (50.0±5.0) mg/male, with half females and half males.

4.2.3 Ants The Yellow House Ant (Monomoriumpharaonis), worker ants raised in the laboratory.

6.3 Test Procedure

6.3.1 Flies A square box apparatus (5.1) was used. The test bait and fly feed were placed diagonally on the bottom of the square box, while a cotton ball soaked in water was placed in the center. In a petri dish and sealed square box, 50 test insects are placed through the insect release hole (F), and the number of dead test insects is recorded after 24 hours. The test should be repeated at least three times. Repeat the experiment and set up a blank control group fed with normal feed. The experimental apparatus should be cleaned at the end of each experiment.

6.3.2 Cockroaches A square box apparatus (5.1) was used. Thirty cockroaches were placed into the sealed square box apparatus through the release hole (F), and the release hole was sealed tightly. The test insects were allowed to recover to normal. After the activity, place the test bait in one corner of the box, place cockroach food in the opposite corner, and place a petri dish containing water-soaked cotton balls in the center. Observe the mortality of the test insects daily. The mortality rate should be recorded, and dead test insects should be removed and counted. Observation should continue until the 12th day after feeding. The test should be repeated at least three times, with normal conditions included. A blank control group fed with feed. The experimental apparatus should be cleaned after each experiment.

6.3.3 Ants An enamel square dish (5.3) was used. A band of 95% ethanol and talc (1.1) suspension was applied to the upper edge of the inner wall of the enamel square dish opening. The mixture was left to stand for a while. After the alcohol has completely evaporated, 100 worker ants were placed in the dish. Once the ants resumed normal activity, the test ants were placed diagonally across the enamel square dish, approximately 50 mm from the edge. The bait and ant feed were placed in a petri dish with a cotton ball soaked in water in the center. Ant mortality was observed daily, and dead ants were removed and counted. Continue observation until day 7 after feeding. The test should be repeated at least three times, with a blank control group fed with normal feed. At the end of each trial, [further details should be provided]. Cleaning test apparatus.

7.Calculation The mortality rate and corrected mortality rate were calculated using formulas (1) and (2), and the results were rounded to two decimal places. The mean of the experimental data was used as statistical data. The mortality rate in the blank control group is < 5%, no correction is needed; the mortality rate in the blank control group is 5%-20%, correction should be performed; the mortality rate in the blank control group is >20%. The experiment is invalid.