GB/T 43628-2023Method for identification of airborne pathogenic microorganism based on metagenomics sequencing (English PDF)

Foreword

This document complies with the provisions of GB/T 1:1-2020 "Standardization Work Guidelines Part 1: Structure and Drafting Rules of Standardization Documents" Drafting:

Please note that some content in this document may be subject to patents: The publisher of this document assumes no responsibility for identifying patents:

This document is proposed and coordinated by the National Technical Committee for Standardization of Biochemical Testing (SAC/TC387):

This document was drafted by: China Institute of Metrology, Shenzhen BGI Life Sciences Research Institute, China Institute of Testing Technology Biological Research Institute, Beijing Huironghe Technology Co:, Ltd:, Nanjing Metrology Supervision and Inspection Institute:

The main drafters of this document: Wang Jing, Pei Na, Li Manli, Fu Boqiang, Zhou Lihua, Li Junhua, Zhang Yongzhuo, Liang Tianzhu, Zheng Jinlin, Ye Deping, Zhu Rui:

Introduction

Detection and health safety of airborne pathogenic microorganisms in daily environments, public places, medical and health environments, and biosafety laboratories Environmental protection is closely related: Pathogenic microorganisms can spread in the air through bioaerosols and cause the spread of infectious diseases: airborne pathogens The accurate identification of microorganisms is of great significance for the prevention and control of infectious diseases and the monitoring and protection of the environment: due to airborne pathogens Microorganisms have characteristics such as lineage diversity, species complexity, and rapid variability, making Acer's high-throughput, fast, and accurate Gene sequencing methods have technical advantages in the identification of pathogenic microorganisms in the air:

In response to the need for standardization of metagenomic sequencing and identification methods for pathogenic microorganisms in the air, this document provides information on important pathogenic microorganisms in the air:

Detection and identification methods are carried out in key aspects such as air microbial aerosol sampling, pre-processing methods, library preparation, sequencing, and data result analysis:

Regulations have been formulated to meet the standardized use of metagenomic sequencing methods for airborne pathogenic microorganisms to improve China’s monitoring of pathogenic microorganisms: ability:

Metagenomic sequencing and identification method of airborne pathogenic microorganisms

1 Scope

This document specifies the test conditions, test procedures, quality control and identification of metagenomic sequencing and identification methods for airborne pathogenic microorganisms:

This document is suitable for metagenomic sequencing identification and monitoring of pathogenic microorganisms such as bacteria, fungi, and viruses in the air:

2 Normative reference documents

GB/T 6682

GB/T 18204

GB 19489

GB/T 30989

GB/T 39990

GB/T 40974

GB 41918

3 Terms and definitions

The following terms and definitions apply to this document: 3:1 Microorganisms that invade organisms and cause infections or even infectious diseases, including bacteria, fungi, viruses, etc: 3:2 metagenome metagenome The sum of the genetic material of all microorganisms in a specific environment, including the genomes of cultivable and uncultivable microorganisms: [Source: JJF1265-2022,4:54] 3:3 Sequencing Determination of the order and composition of the nucleotide bases (adenine, guanine, cytosine, thymine or uracil) in nucleic acid molecules:

Note: Sequences are usually expressed from 5' end to 3' end:

[Source: ISO 20397-2:2021,3:19] 3:4 Library A group of nucleotide submolecules obtained from biological sources, artificial synthesis or cloning technology, such as genomic libraries and complementary DNA library: