Valid

GB/T 43628-2023Method for identification of airborne pathogenic microorganism based on metagenomics sequencing (English PDF)

空气中病原微生物宏基因组测序鉴定方法

Preview PDF

This is a limited preview

Buy now to download the full PDF (17 pages)

Need quotation for your management?

Get a formal quotation for GB/T 43628-2023 in 30 minutes.

Issued by

SAMR; SAC

Level / Type

National · Recommended

Issue date

December 28, 2023

Implementation date

July 1, 2024

Scope

GB/T 43628-2023 is the English-translated version of 空气中病原微生物宏基因组测序鉴定方法.

GB/T 43628-2023 is the Chinese standard "Method for identification of airborne pathogenic microorganism based on metagenomics sequencing".

Its scope clause reads: This document specifies the test conditions, test procedures, quality control and identification of metagenomic sequencing and identification methods for airborne pathogenic microorganisms: This document is suitable for metagenomic sequencing identification and monitoring of pathogenic microorganisms such as bacteria, fungi, and viruses in the air:. Its clauses include normative reference documents; terms and definitions.

Found what you need?

Document preview — GB/T 43628-2023

National Standard of the People's Republic of China

ICS: 07.080
Classification: A 40

Issued by: State Administration for Market Regulation; Standardization Administration of the PRC

Preface
Introduction
1 Scope
2 Normative references
3 Terms and Definitions
4 Abbreviations
5 Principle
6 Reagents
7 Instruments and Equipment
8 Test step
9 Quality Control
10 Identification of airborne pathogenic microorganisms
Appendix A (informative) Nucleic acid extraction method
Appendix B (informative) Sequencing process
Appendix C (informative) Reference genome database and selection of pathogenic microorganisms
Appendix D (informative) Example 10 of comparative analysis of Yersinia pestis and SARS virus sequencing data

1 Scope

This document specifies the test conditions, test procedures, quality control and identification of metagenomic sequencing and identification methods for airborne pathogenic microorganisms:

This document is suitable for metagenomic sequencing identification and monitoring of pathogenic microorganisms such as bacteria, fungi, and viruses in the air:

2 Normative reference documents

The contents of the following documents constitute essential provisions of this document through normative references in the text: Among them, the dated quotations

For undated referenced documents, only the version corresponding to that date applies to this document; for undated referenced documents, the latest version (including all amendments) applies to this document:

GB/T 6682 Specifications and test methods for water used in analytical laboratories

GB/T 18204:3 Hygienic inspection methods for public places Part 3: Airborne microorganisms

GB 19489 General requirements for laboratory biosafety

GB/T 30989 High-throughput gene sequencing technical regulations

GB/T 39990 Technical conditions for particle bioaerosol sampler

GB/T 40974 Nucleic acid sample quality evaluation method

GB 41918 biological safety cabinet

WS589 Pathogenic Microbiology Laboratory Biosafety Label

3 Terms and definitions

The following terms and definitions apply to this document:

3:1

Microorganisms that invade organisms and cause infections or even infectious diseases, including bacteria, fungi, viruses, etc:

3:2 metagenome metagenome

The sum of the genetic material of all microorganisms in a specific environment, including the genomes of cultivable and uncultivable microorganisms:

[Source: JJF1265-2022,4:54]

3:3

Sequencing

Determination of the order and composition of the nucleotide bases (adenine, guanine, cytosine, thymine or uracil) in nucleic acid molecules:

Note: Sequences are usually expressed from 5' end to 3' end:

[Source: ISO 20397-2:2021,3:19]

3:4

Library

A group of nucleotide submolecules obtained from biological sources, artificial synthesis or cloning technology, such as genomic libraries and complementary

DNA library:

GB/T 44830 | GB/T 40179 | GB/T 40267 | GB/T 47334 | GB/T 43629.1 | […]

......
This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 17 pages — is available in the English PDF.

Referenced standards

Normative references

GB/T 18204

Editions of GB/T 43628

Edition	Title	Revision	Status
GB/T 43628-2023	Method for identification of airborne pathogenic microorganism based on metagenomics sequencing	current edition	Current

This page sells the current edition, GB/T 43628-2023. Earlier editions are listed for reference only.

How to Download GB/T 43628-2023

1
Add to cart
Click "Download PDF" on this page and choose "Buy this standard". You can add more standards before checkout.
2
Checkout
Enter your email and billing details. Payment is processed securely by Stripe (cards or bank transfer). Prefer bank details? Request a quotation (min. order $300).
3
Instant delivery (0–9 sec)
Delivery is automatic: within seconds of payment you'll receive an email with a secure download link. The link stays valid for 72 hours.
4
Invoice included
A tax invoice is attached to the confirmation email. Need a custom invoice? Contact us.