GB/T 30989-2014Technical regulation of high-throughput gene sequencing (English PDF)

1 Scope

This standard specifies the terms and definitions of high-throughput gene sequencing techniques, principle sequencing Technical indicators related to the main instrument, sequencing reagents

And it requires preparation and sequencing methods.

This standard applies to animal tissues, blood, feces, oral mucosa, hair, plant tissue, soil, microorganisms and other biological samples high

Throughput gene sequencing.

This standard applies to unidirectional sequencing, direct sequencing, sequencing of the three types of mixed sample sequencing, expression profiling also apply to sequencing, ChIP test

Sequence, Cancer Research and parting, miRNA sequenced microbial genomes element, the type of pathogen detection, immunological and immunological diseases work, methylated

Sequencing, sequence polymorphism detection, clinical genetic diagnosis, personalized sequencing and genome sequencing.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein

Member. For undated references, the latest edition (including any amendments) applies to this document.

Laboratory use specifications and test methods GB/T 6682 Analysis

General requirements GB/T 27025-2008 testing and calibration laboratories

JB/T 6777 UV-Vis spectrophotometer

YY/T 0087 electrophoresis apparatus

YY0569 II level biological safety cabinet

YY/T 0657 Medical Centrifuge

YY91037 electric heated water bath

ASTME1342-1997 Save bacteria, fungi with freezing, freeze-drying and low temperature curing method, protists, viruses, genetic factors

And standard animal and plant tissues Practice (StandardPracticeforPreservationbyFreezing, Freeze-Drying, andLowTemperatureMaintenanceofBacteria, Fungi, Protista, Viruses, GeneticElements, andAn- imalandPlantTissues)

3 Terms and Definitions

The following terms and definitions apply to this document.