GB/T 40974-2021Quality evaluation methods of the nucleic acid (English PDF)
核酸样本质量评价方法
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Issued by
SAMR; SAC
Level / Type
National · Recommended
Issue date
November 26, 2021
Implementation date
June 1, 2022
Scope
GB/T 40974-2021 is the English-translated version of 核酸样本质量评价方法.
GB/T 40974-2021 sets out the Chinese national methods for evaluating the quality of nucleic acid samples. For DNA samples it covers how to assess concentration, purity and integrity, and it does the same for RNA samples, so that a laboratory can judge whether an extracted sample is fit for further analysis.
It applies to nucleic acid samples of human, animal, plant and microbial origin, but not to small-molecule nucleic acid samples. The standard also defines the basic terms it relies on, including nucleotide, nucleic acid, deoxyribonucleic acid (DNA), ribonucleic acid (RNA) and plasmid.
Biobanks, clinical and research laboratories and sample providers use it. It was issued on 26 November 2021 and has applied since 1 June 2022.
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Document preview — GB/T 40974-2021
National Standard of the People's Republic of China
- ICS
- 07.080
- Classification
- C 40
Issued by: State Administration for Market Regulation; Standardization Administration of the PRC
Contents
- Foreword
- 1 Scope
- 2 Normative references
- 3 Terms and definitions
- 4 Quality evaluation method
- 4.1 DNA sample
- 4.1.1 DNA concentration
- 4.1.2 DNA purity
- 4.1.3 DNA integrity
- 4.2 RNA sample
- 4.2.1 RNA concentration
- 4.2.2 RNA purity
- 4.2.3 RNA integrity
Foreword
This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for Standardization Work Part 1: Structure and Drafting Rules of Standardization Documents" Drafting.
1 Scope
This document describes the quality assessment methods for the concentration, purity and completeness of nucleic acid samples.
This document is applicable to the quality evaluation of nucleic acid samples from human, animal, plant, and microbial sources.
This document is not applicable to the quality evaluation of small molecule nucleic acid samples.
2 Normative references
There are no normative references in this document.
3 Terms and definitions
The following terms and definitions apply to this document.
3.1 Nucleotide
The phosphate ester of nucleoside is the basic unit of nucleic acid.
Note: The connection position is different, there are 2'-nucleotide (nucleoside-2'-phosphate), 3'-nucleotide (nucleoside-3'-phosphate), 5'-nucleotide (nucleoside-5 '-Phosphoric acid) three kinds.
3.2 Nucleic acid
A class of biological macromolecules connected by nucleotides or deoxynucleotides through 3',5'-phosphodiester bonds. Very important creatures The function is mainly to store heritage information and transmit genetic information. Including two types of ribonucleic acid and deoxyribonucleic acid.
3.3 Deoxyribonucleic acid; DNA
Biological macromolecules with genetic information. It consists of four main deoxynucleotides (deoxy adenine monophosphate dAMP, deoxy monophosphate Guanine dGMP, deoxycytosine monophosphate dCMP and deoxythymine monophosphate dTMP) are connected by 3',5'-phosphodiester bonds. become.
Note: Their composition and arrangement are different, showing different biological functions, such as. coding function, copy and transcription regulation functions.
3.4 Ribonucleic acid; RNA
A type of nucleic acid. A polymer composed of nucleotides connected by 3',5'-phosphodiester bonds.
Note: Different types of RNA have different chain lengths and perform various biological functions. For example, RNA related to protein biosynthesis has messenger RNA (mes-sengerRNA, mRNA), transfer RNA (transferRNA, tRNA) and ribosomal RNA (ribosomeRNA, rRNA); and post-transcription Work-related RNAs include small nuclear RNA (smalnuclearRNA, snRNA), small nucleolar RNA (smalnucleolarRNAs, snoRNAs); and The RNA related to biological regulation includes microRNAs (miRNA), small interfering RNA (smalinterferingRNA, siRNA) and so on.
3.5 Plasmid
A self-replicating circular double-stranded DNA molecule in bacterial cells that can stably exist outside the chromosomes and be passed on to the offspring. It is generally not integrated into the host chromosome.
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This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 15 pages — is available in the English PDF.
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