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GB/T 42117-2022Diagnostic techniques for ovine Theileria infection (English PDF)

羊泰勒虫病诊断技术

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Issued by

SAMR; SAC

Level / Type

National · Recommended

Issue date

December 30, 2022

Implementation date

July 1, 2023

Scope

GB/T 42117-2022 is the English-translated version of 羊泰勒虫病诊断技术.

GB/T 42117-2022 sets out how theileriosis in sheep is diagnosed. It fixes the technical requirements for the clinical diagnosis and for the overall judgement, and describes two laboratory methods for the two Theileria species it names: microscopic examination of a blood smear, and a polymerase chain reaction test.

Its clinical clause lists the typical signs - depression, a persistent fever with the body temperature rising to 40 °C to 42 °C, conjunctival congestion and swelling, anaemia or yellowing of the mucous membranes, swollen, firm and tender prescapular and prefemoral lymph nodes, weight loss, and light to dark yellow urine without blood.

It covers pathogen detection, latent infection and clinical cases, requires the sampling and testing work to comply with GB 19489 on laboratory biosafety, and quotes GB/T 6682. Issued on 30 December 2022, it has applied since 1 July 2023.

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Document preview — GB/T 42117-2022

National Standard of the People's Republic of China

ICS
11.220
Classification
B 41

Issued by: State Administration for Market Regulation; Standardization Administration of the PRC

Contents

  • Foreword
  • 1 Scope
  • 2 Normative references
  • 3 Terms and Definitions
  • 4 Abbreviations
  • 5 Biosafety Requirements
  • 6 Clinical Diagnosis

Foreword

This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for Standardization Work Part 1: Structure and Drafting Rules for Standardization Documents" drafting.

1 Scope

This document specifies the technical requirements for the clinical diagnosis and comprehensive judgment of sheep Theileriasis, and describes the pathogen of sheep Theileriasis (Theileria reuei) and Theileria yoshii) blood smear microscopy detection and polymerase chain reaction detection method.

This document is applicable to the detection of the pathogen of sheep Theileria, recessive infection of Theileria and diagnosis of clinical cases.

2 Normative references

The content in the following documents constitutes the essential provisions of this document through normative references in the text, among which, the dated references For documents, only the version corresponding to the date is applicable to this document; for undated reference documents, the latest version (including all amendments) is applicable to this document.

GB/T 6682 Analytical test water specifications and test methods

GB 19489 General Requirements for Laboratory Biosafety

3 Terms and Definitions

This document does not have terms and definitions that need to be defined.

4 Abbreviations

The following abbreviations apply to this document. TAE. Nucleic acid electrophoresis buffer (Tris-Acetic acid-EDTA)

5 Biosafety Requirements

The experimental operations involved in the sample collection, processing and testing process shall comply with the relevant provisions of GB 19489.

6 Clinical Diagnosis

6.1 Typical clinical symptoms

6.1.1 The animal is depressed, and its body temperature rises to 40°C~42°C, showing persistent fever.

6.1.2 Conjunctival hyperemia and swelling, anemia or visible yellow staining of mucous membrane.

6.1.3 The anterior shoulder and anterior femoral lymph nodes are swollen, firm and sensitive to palpation.

6.1.4 Weight loss, anemia, light yellow or dark yellow urine, but no hematuria.

......
This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 15 pages — is available in the English PDF.

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