GB/T 39884-2021Examination methods for three components in cannabis in suspected drugs—Gas chromatography and gas chromatography-mass spectrometry (English PDF)

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009.

1 Scope

This standard specifies the qualitative inspection method and gas phase method for the effective ingredients in the solid samples of suspected drugs by gas chromatography-mass spectrometry (GC-MS)

Principles, reagents and materials, instruments and equipment, operating methods, and result evaluation and expression of chromatographic (GC) quantitative inspection methods.

This standard applies to the qualitative analysis of THC, cannabidiol and cannabidiol in solid samples of suspected drugs, and the determination of THC in solid samples. Quantitative analysis.

2 Normative references

The following documents are indispensable for the application of this document. For dated reference documents, only the dated version applies to this document. For undated reference documents, the latest version (including all amendments) is applicable to this document.

GB/T 27418 Evaluation and Expression of Uncertainty in Measurement

GA/T 122 Terminology for toxicological analysis

3 Terms and definitions

The terms and definitions defined in GA/T 122 are applicable to this document.

4 Principle

Extract the tetrahydrocannabinol, cannabidiol, and cannabidiol in solid samples of suspected drugs. Use gas chromatography-mass spectrometry to detect The retention time, characteristic ion fragments and ion abundance ratio are used as the basis for qualitative judgment; gas chromatography is used for detection, and the chromatographic peak area is used as the quantitative basis.

According to the data, quantitative analysis was carried out using external standard single point method or internal standard standard curve method.

5 Reagents

Unless otherwise specified, the reagents used in the analysis are chromatographically pure.

5.3 Standard substance stock solution. 0.1mg/mL tetrahydrocannabinol standard stock solution, 1.0mg/mL cannabinol standard stock solution, 1.0mg/mL large The standard stock solution of ephedrine can be stored under refrigeration at 0°C~4°C, and the validity period is 12 months.

5.4 Internal standard stock solution for quantification by internal standard standard curve method. Weigh an appropriate amount of tribenzylamine, make the volume with methanol, and prepare 0.5mg/mL tribenzylamine.

5.5 Standard working solution for qualitative use. pipette THC standard stock solution, cannabidiol standard stock solution, and cannabidiol standard stock solution respectively Each appropriate amount was diluted with methanol to prepare a mixed standard working solution of tetrahydrocannabinol, cannabidiol, and cannabidiol, all 0.05mg/mL. Store under refrigeration at 0°C~4°C, valid for 1 month.

5.6 Tetrahydrocannabinol qualitative control standard working solution. pipette an appropriate amount of the tetrahydrocannabinol standard substance stock solution, dilute with methanol, and prepare