GB/T 35808-2018Method for analysis of forestry biomass—Determination of cellulase activity (English PDF)

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009.

1 Scope

This standard specifies the terms and definitions, principles, reagents and materials, apparatus and instruments used in the determination of cellulase activity under given conditions And equipment, standard curve drawing, sample preparation, measurement procedures, calculation of results, repeatability requirements, test reports.

This standard applies to the determination of cellulase activity in forestry biomass materials analysis.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version applies to this document. For undated references, the latest edition (including all amendments) applies to this document.

GB/T 6682 analysis of experimental water specifications and test methods

GB/T 30366-2013 Biomass terms

3 Terms and definitions

GB/T 30366-2013 defined and the following terms and definitions apply to this document. For ease of use, the following list is repeated GB/T 30366-2013 some of the terms and definitions.

4 Principle

Cellulase at a certain temperature and pH conditions, the hydrolysis of carboxymethyl cellulose produced by cellobiose, glucose and other reducing sugars can be alkaline Under the conditions of 3,5-dinitrosalicylic acid (DNS) reduction, the generation of brown-red amino compounds, maximum absorption at 540nm wavelength, The amount of reducing sugar produced by enzymolysis within a certain range is proportional to the absorbance of the reaction solution, and the amount of reducing sugar produced can be measured by a colorimetric method Cellulase activity was calculated.

5 Reagents and materials

5.1 All reagents used in this standard unless otherwise specified, are of analytical grade, water are in line with the provisions of GB/T 6682 secondary water.