GB/T 33839-2017Methods of transmission electron microscope for biological specimen containing carbon nanomaterials involving biological effect (English PDF)

Foreword

This standard is drafted in accordance with the rules given in GB/T 1.1-2009.

Introduction

It is unclear whether the widely used carbon-based nanomaterials will pose a hazard to biological systems. The main reason there are three. one is the The biological effects of carbon-based nanomaterials on a scale are not clear, including. whether they can enter the organism, how to enter the organism, into the organism Whether it affects the function of the organism; the second is to enter the biological nano-materials testing the lack of adequate technical and test standards; Third, nano-materials There is no standard to be tested for the safety effects of the organism, resulting in the clarity of the safety of the nanomaterials to the organism. Therefore, the development of these three The standard system is an important basis for nanomaterials in life science research.

There are many ways to study the biological effects of carbon-based nanomaterials, but the ultrastructures of cells are more intuitive by electron microscopy.

Therefore, the study of carbon-based nano-materials on biological tissue cells and even the biological structure of the impact of the current or mainly based on electron microscopy The relevant detection standard is the key to the application of carbon-based nanomaterials in biomedicine. In order to standardize the preparation of carbon-based nanomaterials biological samples Procedures, the correct guidance of transmission electron microscopy detection work, it is necessary to develop this standard.

1 Scope

This standard specifies the use of transmission electron microscopy (hereinafter referred to as transmission electron microscopy) detection of carbon-containing nano-materials containing biological samples of the ultrastructure The technology and norms.

This standard is applicable to the detection and detection of bio-thin specimens of carbon-based nanomaterials by transmission electron microscopy.

2 Normative reference documents

The following documents are indispensable for the application of this document. For dated references, only the dated edition applies to this document. For undated references, the latest edition (including all modifications) applies to this document.

GB/T 18873-2008 Transmission electron microscopy of biofilm samples - Quantitative analysis of X-ray energy spectrum

GB/T 19619-2004 Glossary of terms for nanomaterials

3 Terms and definitions

GB/T 19619-2004 Definitions and the following terms and definitions apply to this document.

4 Principle of the method

The surface physical and chemical properties of the carbon-based nanomaterials make them easy to form agglomerate, which is dispersed and contacted with the organism. Carbon - based nanomaterials and organisms After exposure, the use of ultra-micro-structure sample preparation method, made of biological thin sample. The carbon nanomaterials were detected by transmission electron microscopy The ultrastructural changes of cells and organelles produced to determine the biological effects of carbon-based nanomaterials on organisms. With high energy electron beam irradiation Biological thin sample of the tiny area, the incident electron beam through the thin sample, the transmission electrons carry a biological thin sample internal information, imaging The ultrastructural image of the bio-thin specimen is formed.