GB/T 18873-2008General guide of transmission electron microscope (TEM)- X-ray energy dispersive spectrometry(EDS) quantitative microanalysis for thin biological specimens (English PDF)
生物薄试样的透射电子显微镜-X射线能谱定量分析通则
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Issued by
SAMR; SAC
Level / Type
National · Recommended
Issue date
June 16, 2008
Implementation date
March 1, 2009
Scope
GB/T 18873-2008 is the English-translated version of 生物薄试样的透射电子显微镜-X射线能谱定量分析通则.
GB/T 18873-2008 is the Chinese standard "General guide of transmission electron microscope (TEM)- X-ray energy dispersive spectrometry(EDS) quantitative microanalysis for thin biological specimens".
Its scope clause reads: This standard specifies the transmission electron microscope -X-ray spectroscopy quantitative analysis of technical requirements and specifications thin biological specimens.
This standard applies to thin biological specimens contained in a transmission electron microscope, non-quantitative X-ray spectrum of light element analysis. Its clauses include terms and definitions; fundamentals; instruments and equipment; thin biological standards for selection.
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Document preview — GB/T 18873-2008
National Standard of the People's Republic of China
- ICS
- 71.040.99
- Classification
- N 53
- Replacing
- GB/T 18873-2002
Issued by: State Administration for Market Regulation; Standardization Administration of the PRC
Contents
- 1 Scope
- 2 Terms and definitions
- 3 Fundamentals
- 4 instruments and equipment
- 5 thin biological standards for selection
- 6 2 thin biological specimens
- 7 Preparation
- 8 Select Instrument Measurement Condition
- 9 Measurement and Analysis Step
- 10 error
- 11 The results of the release
- Appendix A (informative) Calculation method of biological tissue factor G
1 Scope
This standard specifies the transmission electron microscope -X-ray spectroscopy quantitative analysis of technical requirements and specifications thin biological specimens.
This standard applies to thin biological specimens contained in a transmission electron microscope, non-quantitative X-ray spectrum of light element analysis.
2 Terms and definitions
The following terms and definitions apply to this standard.
2.1
Means cut using a microtome to a thickness of 50nm ~ 300nm in the biological sample.
2.2
Means cut using a microtome to a thickness of 50nm ~ 300nm biological sample of a standard.
2.3
Biological samples and biological standards of chemical composition of elements factor. Available factor G of formula (1) is calculated.
G = sigma
Where.
3 Fundamentals
With a high energy electron beam irradiation thin biological specimens micro areas of focus in the region contained the elements of atomic excitation by high-energy electron beam
Send produce characteristic X-ray, which corresponds to the characteristic X-ray energy-related elements; integrated intensity of characteristic X-rays corresponding to the concentration of the element.
Spectrometer using a sample of the received characteristic X-ray peak intensity and background intensity (i.e., a continuous X-ray intensity) difference under the same conditions and
Characteristics of electron beam irradiation to obtain thin biological standards for X-ray peak intensity difference between background intensity determined by comparing the measured excitation thin biological specimens
The content of each element within the region.
4 instruments and equipment
--- Transmission electron microscope;
--- X-ray spectrometer;
--- Ultramicrotome.
5 thin biological standards for selection
5.1
thin biological standards for chemical composition should be as similar as possible with thin biological samples and chemical composition constant.
5.2
thin biological standards for the thickness of the analyzed thickness thin biological specimens should be similar, recommended ultramicrotome into a thickness less than […]
......
This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 15 pages — is available in the English PDF.
Referenced standards
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Editions of GB/T 18873
| Edition | Title | Revision | Status |
|---|---|---|---|
| GB/T 18873-2008 | General guide of transmission electron microscope (TEM)- X-ray energy dispersive spectrometry(EDS) quantitative microanalysis for thin biological specimens | current edition | Current |
| GB/T 18873-2002 | General guide of transmission electron microscope (TEM)- X-ray energy dispersive spectrometry(EDS) quantitative microanalysis for thin biological specimens | previous edition | Superseded |
This page sells the current edition, GB/T 18873-2008. Earlier editions are listed for reference only.
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