GB/T 47187-2026Microbeam analysis - Volume electron microscopy - FIB-SEM imaging method for the 3D structure of biological samples (English PDF)

3.1

By performing continuous ultrathin cutting on millimeter-scale samples, and then collecting sequential electrical data from the obtained continuous ultrathin slices or continuously exposed surfaces, the following methods were employed.

This technique involves reconstructing high-resolution, large-scale three-dimensional images to obtain three-dimensional structural information within a certain volume of a sample.

3.2 Focused ion beam (FIB)

A focused ion beam is used to bombard the sample surface, and the scanning or processing trajectory, step size, and dwell time of the ion beam are controlled by a computer.

The system integrates intervals and cycle counts to enable the imaging, etching, induced deposition, and implantation of materials for analytical processing.

[Source. GB/T 45468-2025, 3.1, with modifications]

3.3

Micro-nano fabrication and imaging instruments with FIB and scanning electron microscopy imaging systems.

Note. This instrument consists of an ion optical system, a pattern generator, an electron optical system, a sample chamber, a signal acquisition and processing system, and a vacuum system.

[Source. GB/T 45468-2025, 3.2, with modifications]

3.4 Heavy metal staining

Biological samples are stained with heavy metal compound staining agents to cause different amounts of heavy metals to adsorb onto cellular structures or macromolecules in the samples.

Metals are used to enhance the image contrast of ultrastructures in samples.