GB/T 31270.18-2014Test guidelines on environmental safety assessment for chemical pesticides—Part 18: Amphibian acute toxicity test (English PDF)

Foreword

GB/T 31270 "Test guidelines on environmental safety assessment for chemical pesticides" is divided into 21 parts.

- Part 1. Transformation in soils;

- Part 2. Hydrolysis;

- Part 3. Phototransformation;

- Part 4. Adsorption/Desorption in soils;

- Part 5. Leaching in soil;

- Part 6. Volatility;

- Part 7. Bioconcentration test;

- Part 8. Degradation in water-sediment systems;

- Part 9. Avian acute toxicity test;

- Part 10. Honeybee acute toxicity test;

- Part 11. Silkworm acute toxicity test;

- Part 12. Fish acute toxicity test;

- Part 13. Daphnia sp. acute immobilisation test;

- Part 14. Alga growth inhibition test;

- Part 15. Earthworm acute toxicity test;

- Part 16. Soil microorganism toxicity test;

- Part 17. Trichogramma acute toxicity test;

- Part 18. Amphibian acute toxicity test;

- Part 19. Effects on non-target plants;

- Part 20. Livestock short-term dietary toxicity test;

- Part 21. Macro-crustacean toxicity test.

This Part is Part 18 of GB/T 31270.

1 Scope

This Part of GB/T 31270 specifies the amphibian acute toxicity test's material, conditions, operation, quality control, data processing, test report and other basic requirements.

This Part is applicable to amphibian acute toxicity test for chemical pesticide registration. Other types of pesticides may refer for use.

This Part is not applicable to volatile and insoluble chemical pesticides.

2 Terms and definitions

The following terms and definitions are applicable to this document.

3 Test overview

The test methods for amphibian acute toxicity are static test, semi-static test, and flow-through test. It shall adopt suitable method according to the nature of the test substance. Respectively prepare different concentrations of the test substances. Observe and record tadpole's symptoms of poisoning and number of deaths every day during 96 h test. And calculate LC50 values and 95% confidence limits of 24 h, 48 h, 72 h and 96 h respectively.

4 Test methods

5 Test report

Test report shall at least contain the following items.

- Information of test substance, including test pesticide's common name, chemical name, structural formula, CAS number, purity, basic physical and chemical properties, origin, etc.;

- Test biology's name, origin, size and domesticated situation;

- Test conditions, including test temperature, illumination, dilution water's temperature, dissolved oxygen concentration and pH, etc.;

- Test liquid concentration, LC50 value of 24 h, 48 h, 72 h, and 96 h, 95% confidence limits, and calculation method;

- If tadpoles of the control group die or have abnormal reactions;

- Observed toxic effects, e.g., any abnormal behavior or poisoning symptom of test tadpoles;

- Classification of amphibian toxicity refers to Appendix B.

Appendix B (Informative)

Acute toxicity classification of pesticide to tadpole According to median lethal concentration LC50 (96h) of pesticide to tadpole, classify the acute toxicity of pesticide to tadpole into four grades, as shown in Table B.1. Table B.1 Toxicity classification of pesticides to amphibian Toxicity classification LC50 (96h) / (mg a.i./L)

Severe toxicity LC50 <= 0.1 High toxicity 0.1 < LC50 <= 1.0 Medium toxicity 1.0< LC50 <= 10 Low toxicity LC50 >10 References [1] NY/T 1667.1-2008 Terminology of pesticide registration management - Part 1: Basic terminology [2] NY/T 1667.2-2008 Terminology of pesticide registration management - Part 2: Product chemistry [3] OECD (2009). Guideline 231. The Amphibian Metamorphosis Assay, OECD Guidelines for the Testing of Chemicals.

[4] US EPA (1996). Tadpole / Sediment Subchronic Toxicity Test (OPPTS 850.1800). Ecological effects test guidelines.

[5] ASTM (2007). Standard Guide for Conducting Acute Toxicity Tests on Test Materials with Fishes, Macr. Oinvertebrates, and Amphibians. American Society for Testing and Materials, ASTM E729-96.

[6] OECD (l992). Guideline 203. Fish, Acute Toxicity Test. OECD Guidelines for the Testing of Chemicals.

[7] Cai Daoji. Research on Pesticide Environmental Toxicology. Beijing. China Environmental Science Press, 1999. […]