GB/T 31270.17-2025Test guidelines on environmental safety assessment for chemical pesticides - Part 17: Trichogramma acute toxicity test (English PDF)

Foreword

This Document was drafted as per the rules specified in GB/T 1.1-2020 Directives for Standardization - Part 1: Rules for the Structure and Drafting of Standardizing Documents.

This Document is Part 17 of GB/T 31270 Test Guidelines on Environmental Safety Assessment for Chemical pesticides. GB/T 31270 published the following parts:

--- Part 1: Soil Metabolism Test;

--- Part 2: Hydrolysis Test;

--- Part 3: Phototransformation;

--- Part 4: Adsorption/Desorption in Soils;

--- Part 5: Leaching Test in Soil;

--- Part 6: Volatility;

--- Part 7: Bioconcentration Test;

--- Part 8: Aquatic Sediment System Metabolism Test;

--- Part 9: Avian Short-Term Dietary Toxicity Test;

--- Part 10: Honeybee Acute Toxicity Test;

--- Part 11: Silkworm Acute Toxicity Test;

--- Part 12: Fish Acute Toxicity Test;

--- Part 13: Daphnia Sp. Acute Immobilisation Test;

--- Part 14: Alga Growth Inhibition Test;

--- Part 15: Earthworm Acute Toxicity Test;

--- Part 16: Soil Microorganism Toxicity Test;

--- Part 17: Trichogramma Acute Toxicity Test;

--- Part 18: Amphibian Acute Toxicity Test;

--- Part 19: Effects on Non-Target Plants;

--- Part 20: Livestock Short-Term Dietary Toxicity Test;

Warning: Personnel using this Document shall have practical experience working in a formal laboratory. This Document does not address all potential safety issues. Users are responsible for taking appropriate safety and health precautions and ensuring compliance with relevant national regulations.

1 Scope

This Document describes the acute toxicity test method of Trichogramma in the environmental safety assessment for chemical pesticides.

This Document applies to acute toxicity testing of Trichogramma for chemical pesticide registration. This Document does not apply to volatile pesticides.

2 Normative References

The provisions in following documents become the essential provisions of this Document through reference in this Document. For the dated documents, only the versions with the dates indicated are applicable to this Document; for the undated documents, only the latest version (including all the amendments) is applicable to this Document.

GB/T 6682 Water for analytical laboratory use - Specification and test methods

NY/T 2882.7 Guidance on environmental risk assessment for pesticide registration - Part 7: Non-target arthropod

3 Terms and Definitions

For the purposes of this Document, the following terms and definitions apply.

4 Principle

The test substance was prepared into a series of test solutions of different concentrations; quantitatively and uniformly added to finger-shaped tubes of a certain area or sprayed onto glass plates or leaf surfaces, and then dried before being transferred to Trichogramma. The number of dead Trichogramma is investigated 24 h after poisoning, and the LR50 and its 95% confidence interval for the Trichogramma are determined.

5 Test Conditions

The test shall be conducted at 25 °C ± 2 °C, relative humidity 60% ~ 80%, and in the dark.

6 Reagents and Materials

7 Apparatus

7.1 Electronic balance (with sensitivity >= 0.0001 g).

7.2 Artificial climate chamber and other culture equipment.

7.3 Pipettes.

7.4 Temperature and humidity monitoring equipment such as temperature and humidity recorders.

7.5 Roller tube machine (suitable for finger tube poisoning method).

7.6 Spraying equipment (uniform spraying, quantitative calculation capability, suitable for glass plate and plant leaf poisoning methods).

8 Test Substance and Reference

9 Test Procedures