GB/T 28642-2012Rapid determination of salmonella spp in animal feeding stuffs - PCR method (English PDF)
饲料中沙门氏菌的快速检测方法 聚合酶链式反应（PCR）法
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Issued by
SAMR; SAC
Level / Type
National · Recommended
Issue date
July 31, 2012
Implementation date
November 1, 2012
Scope
GB/T 28642-2012 is the English-translated version of 饲料中沙门氏菌的快速检测方法 聚合酶链式反应（PCR）法.
GB/T 28642-2012 is the Chinese standard "Rapid determination of salmonella spp in animal feeding stuffs - PCR method". Its scope clause reads: This standard specifies the PCR method for rapid detection of Salmonella in feed for.
This standard applies to feed Salmonella qualitative screening. Its clauses include reagents and materials.
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Document preview — GB/T 28642-2012
National Standard of the People's Republic of China
- ICS
- 65.120
- Classification
- B 46
Issued by: State Administration for Market Regulation; Standardization Administration of the PRC
Contents
- 1 Scope
- 2 Normative references
- 4 Reagents and materials
- 4.2 PremixTaq buffer (2 ×)
- 4.3 agarose
- 4.5 buffered peptone water (BPW)
- 4.6 Soy peptone broth (RVS)
- 4.7 selenite cystine enrichment broth (SC)
- 4.9 Quality control strains
1 Scope
This standard specifies the PCR method for rapid detection of Salmonella in feed for.
This standard applies to feed Salmonella qualitative screening.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated edition applies to this article
Member. For undated references, the latest edition (including any amendments) applies to this document.
Laboratory use specifications and test methods GB/T 6682 Analysis
GB/T 13091 salmonella in feed detection method
GB/T 14699.1 feed samples
GB 19489 Laboratory Biosafety General Requirements
SN/T1193 genetic testing laboratory technical requirements
SN/T1870 pathogens in food real-time PCR detection methods
Principle 3
Use boiling water bath so that bacterial cells rupture, releasing genomic DNA, centrifugation cell wall visible precipitate in the supernatant as a template
Amplification and agarose gel electrophoresis to detect PCR amplification products.
4 Reagents and materials
Unless otherwise specified, reagents were analytical grade or biochemical reagents, test water meet the requirements of GB/T 6682's.
4.1
Salmonella detection primer (pair) sequence.
SalF. 5'-TCGCACCGTCAAAGGAACCGTAAAGC-3 '
SalR. 5'-GCATTATCGATCAGTACCAGCCGTCT-3 '
4.2 PremixTaq buffer (2 ×)
contains TaqDNA polymerase 1.25U/25µL, 4 mmolMg2, dNTP
Each 0.4mmol.
4.3 agarose
electrophoresis grade.
4.4
Marker2000.
4.5 buffered peptone water (BPW)
See A.1.
4.6 Soy peptone broth (RVS)
See A.2.
4.7 selenite cystine enrichment broth (SC)
See A.3.
4.9 Quality control strains
Salmonella positive standard strains.
......
This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 15 pages — is available in the English PDF.
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