GB/T 26517-2011Determination of 24 preservatives in cosmetics by high performance liquid chromatography method (English PDF)

4.1 Methanol

chromatographically pure.

4.2

salicylic acid, purity of not less than 99.0%; ethyl 4-hydroxybenzoate, purity of not less than 99.0%; propyl 4-hydroxybenzoate, the purity is not

Less than 99.0%; 4-hydroxybenzoic acid ester, a purity of not less than 99.0%; methyl 4-hydroxybenzoate, purity of not less than 99.0%; methyl isobutyl chloro

Thiazolidinone, purity of not less than 99.0%; methylisothiazolinone, purity of not less than 99.0%; benzyl alcohol, purity of not less than 99.0%; phenoxy

Ethanol, purity of not less than 99.0%; p- chloro -m- cresol, purity of not less than 98.0%; triclosan, purity of not less than 97.0%; triclocarban,

A purity of not less than 99.0%; benzoate, a purity of not less than 99.0%; ethyl benzoate, purity of not less than 99.0%; phenyl benzoate, pure

Not less than 99.0%; 2-bromo-2-nitropropane-1,3-diol, a purity of not less than 98.0%; 2,4-dichloro-3,5-dimethylphenol, purity of not less than

99.0%; 4-hydroxy benzoate, purity of not less than 99.0%; o- phenylphenol, purity of not less than 98.0%; chlorine xylenol, purity is not

Less than 98.0%; 4-hydroxy benzoate, isobutyl purity of not less than 99.0%; benzyl chlorophenol, purity of not less than 99.0%; p purity not less

To 99.0%; sorbic acid, purity of not less than 99.0%.

Note. 2,4-dichloro-3,5-di-p-cresol are not included in the supervision Wei Fa [2007] No. 1 "Hygienic Standard for Cosmetics" Table 4 "cosmetic components restricted preservative" in.

4.3 preservative Standard stock solution

Accurately weigh each preservative (4.2) 0.5g, accurate to 0.0001g, were placed in 50mL beaker, add

An appropriate amount of methanol dissolved and transferred to 100mL volumetric flask, diluted with methanol to the mark, mix, that was 5000mg/L of each standard stock preservative

Preparation of liquid.

4.4 preservative mixed standard stock solution

Pipette are standard stock solution of 2mL (4.3) to 100mL volumetric flask with methanol to

Scale, that was each preservative concentration was 100mg/L mixed standard stock solution. Preserve refrigerator can be used for three months.

4.5 Preservatives standard solution

methanol mixed standard stock solution (4.4) respectively dubbed a series of concentration of 0.5µg/mL,

1µg/mL, 5µg/mL, 10µg/mL, the standard working solution 20µg/mL, and stored in the refrigerator, may be used one week.

0.001g, in a 50 mL beaker, dissolved in water and transferred to 1000 mL volumetric flask with water to volume, mix, that was

GB/T 29679-2013 | GB/T 29665-2013 | GB/T 26516-2011 | GB/T 29680-2026 | GB/T 26513-2023 | […]