GB/T 23195-2008Method for the determination of catalase in bee pollen - Ultaraviolet spectrophotometry (English PDF)

1 Scope

This standard specifies the method for determination of bee pollen of catalase.

This standard is applicable by measuring catalase in bee pollen.

2 Normative references

The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent

Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research

Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard.

GB/T 6682 analytical laboratory use specifications and test methods (GB/T 6682-2008, ISO 3696. 1987, MOD)

Principle 3

Hydrogen peroxide absorbs strongly at a wavelength of 240nm, catalase can decompose hydrogen peroxide, the reaction solution in a certain range of absorbance

Inner circle with the reaction time reduced, can be determined according to the rate of change in absorbance measured catalase activity.

4 Reagents

Unless otherwise indicated in the analysis using only analytical reagent and in line with GB/T 6682 requirements distilled or deionized water.

5 Equipment

6 Preparation of test solution

Weigh 1g sample to the nearest 1mg, has been placed with the right amount of liquid nitrogen ice-cold porcelain mortar while adding liquid nitrogen side grinding, the sample to be fully RESEARCH […]