GB/T 22487-2008Principle of aquafeed safety evaluation - Acute toxicity test (English PDF)

3.1

The test substance is dissolved in a certain concentration gradient diluted in water and then placed in the test of aquatic animals.

3.2

The test substance once or several times or feed fed to test animals.

3.3

The test was dubbed a certain concentration of the solution, according to the weight of the test animals with a syringe directly into the test solution for a certain amount of abdominal or

Intramuscular injection.

3.4

After contact toxicity, causing animal mortality rate was 50% water concentration of the test substance, the concentration is an estimated value derived through statistics.

The unit is the amount of test substance per liter of water, in milligrams per liter (mg/L) or grams per liter (g/L) indicated.

3.5

After injection or orally administered test substance is expected within a certain period of time can cause the test substance dose mortality rate of 50%. The dose

After the statistical estimates derived. The unit is the amount of test substance per kg of body weight or injected into the intake, in milligrams per kilogram of body weight (Mg/kg body weight), grams per kilogram (g/kg body weight) or milliliters per kilogram of body weight (mL/kg body weight) indicated.

3.6

After using the maximum concentration and maximum administered orally or by injection capacity of at least 10 test aquatic animals, continuous observation 7d ~ 14d, no […]