GB/T 19167-2020Diagnostic techniques for infectious bursal disease (English PDF)

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009.

This standard replaces GB/T 19167-2003 "Infectious Bursal Disease Diagnosis Technology". Compared with GB/T 19167-2003, except for editorial amendments In addition, the main technical changes are as follows:

---Unify "infectious bursal disease" and "infectious bursal disease" as "infectious bursal disease";

---Added normative reference documents (see Chapter 2);

---Added abbreviations (see Chapter 3);

---Added equipment and reagents (see Chapter 5, Chapter 6, Chapter 7, Chapter 8, Chapter 9);

---Added clinical diagnosis (see Chapter 4);

---Added laboratory diagnostic sample collection (see Chapter 5);

--- Deleted the direct immunofluorescence method (see 2.5.2 of the 2003 edition);

--- Deleted the enzyme-linked immunosorbent assay (see Chapter 5 of the 2003 edition);

---Added RT-PCR detection method (see Chapter 7, Appendix C, Appendix D);

---Added real-time fluorescent RT-PCR detection method (see Chapter 8, Appendix E);

--- The detection of serum antibodies has been deleted (see Chapter 4 of the 2003 edition);

---Added cell culture isolation virus detection method (see 9.4.1, Appendix F);

--- Increased the comprehensive judgment of the diagnosis result (see Chapter 10).

Please note that certain contents of this document may involve patents. The issuing agency of this document is not responsible for identifying these patents.

Introduction

Infectious bursaldisease (IBD), also known as Gumborodisease, is composed of double-stranded RNA Virus (Birnaviridae) avian double-stranded RNA virus (Avibirnavirus) caused by infectious bursal disease virus (IBDV). Turkey, Duck, guinea fowl and ostrich can all be infected with IBDV. Chickens aged 3 to 6 weeks have acute and severe disease and have a high mortality rate, while chickens aged 1 to 3 weeks Often presents subacute or subclinical symptoms. The virus invades the bursa of fabric can cause severe immunosuppression. It is known that IBDV has two serotypes, namely Serotypes 1 and 2, only type 1 is pathogenic to chickens, turkeys and ducks are subclinical infections, and type 2 is not found to be pathogenic.

This disease has characteristic clinical symptoms and eye pathological changes. A preliminary diagnosis can be made. The diagnosis should be confirmed by pathogen detection.

After 2003, the rapid development of IBD diagnostic technology at home and abroad. Some more convenient and accurate new diagnostic techniques have become IBD diagnosis An important means of interruption and prevention. The purpose of this revision of IBD diagnostic technology is twofold. First, it is basically consistent with international standards and changes the country. Domestic standards are significantly lagging behind international standards; the second is to incorporate some new technologies that have been proven to be mature and feasible through years of practice at home and abroad.

In the coming years, we will change the condition that the detection technology in the original standard is less and significantly lags behind the actual application. Revising this standard is conducive to the diagnosis of IBD in my country And prevention is also conducive to enhancing the authority and practicality of national standards.

This standard refers to the OIE "Terrestrial Animal Diagnostic Test and Vaccine Standard Manual" (2016 Edition), and combines the new research results of related technologies in my country Make revisions.

1 Scope

This standard specifies the technical requirements for clinical diagnosis and laboratory diagnosis of infectious bursal disease.

This standard applies to the diagnosis, detection, monitoring and epidemiological investigation of chicken infectious bursal disease. Agar gel immunodiffusion test Test, reverse transcription polymerase chain reaction (RT-PCR), real-time fluorescent reverse transcription polymerase chain reaction (real-time fluorescent RT-PCR), virus analysis The isolation diagnosis method is suitable for the detection of clinical suspected samples and virus separation, and the agar gel immunodiffusion test method is also suitable for immune antibodies Level detection.

2 Normative references

The following documents are indispensable for the application of this document. For dated reference documents, only the dated version applies to this document. For undated references, the latest version (including all amendments) applies to this document.

GB 19489 Laboratory Biosafety General Requirements

3 Abbreviations

The following abbreviations apply to this document.

AGID. Agargel immunodiffusion test (Agargelimmunodiffusiontest)

CEF. Chickembryofibroblastcel

CPE. Cytopathiceffect

IBD. Infectious Bursaldisease

IBDV. Infectious bursaldisease virus

PBS. Phosphate buffered saline (Phosphatebufferedsaline)

PCR. Polymerase chain reaction (Polymerasechain reaction)

RT-PCR. Reverse transcription-polymerase chain reaction (Reversetranscription-polymerasechain reaction)

Real-time RT-PCR. Real-time fluorescent reverse transcription polymerase chain reaction (Real-timereverse transcription-polymerase chainreaction)

SPF. No specific pathogen (Specificpathogenfree)

TAE. Trihydroxymethylaminomethane-acetic acid-ethylenediaminetetraaceticacid)

4 Clinical diagnosis