GB/T 18648-2020Diagnostic techniques for African swine fever (English PDF)

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009.

This standard replaces GB/T 18648-2002 "African swine fever diagnostic technology", compared with GB/T 18648-2002, except for editorial changes In addition, the main technical changes are as follows:

---Added abbreviations (see Chapter 3);

---Added biosecurity measures (see Chapter 4);

---Added clinical diagnosis (see Chapter 5);

---Added laboratory diagnostic sample collection and processing (see Chapter 6);

---Added the fluorescent PCR method (see Chapter 8), the fluorescent RAA method (see Chapter 9), and the highly sensitive fluorescent immunoassay method (see Chapter 10). Chapter), sandwich ELISA antigen detection method (see Chapter 11), blocking ELISA antibody detection method (see Chapter 13), sandwich ELISA ELISA antibody detection methods (see Chapter 14), indirect immunofluorescence methods (see Chapter 15) and other laboratory diagnostic methods;

--- Added comprehensive judgment (see Chapter 16);

---Added the preparation method of sample preservation solution (see Appendix A) and the preparation method of polymerase chain reaction solution (see Appendix B);

---Modified the preparation method of enzyme-linked immunosorbent assay solution (see Appendix C, Appendix C of the 2002 edition).

Please note that certain contents of this document may involve patents. The issuing agency of this document is not responsible for identifying these patents.

1 Scope

This standard specifies the clinical diagnosis of ASF, the collection and processing of laboratory diagnostic samples, as well as common PCR methods and fluorescent PCR methods. Method, fluorescent RAA method, high-sensitivity fluorescent immunoassay method, sandwich ELISA antigen detection method, indirect ELISA antibody detection method, blocking Laboratory diagnostic methods such as ELISA antibody detection methods, sandwich ELISA antibody detection methods, and indirect immunofluorescence methods.

This standard applies to the diagnosis and monitoring of ASF in domestic pigs and wild boars.

2 Normative references

The following documents are indispensable for the application of this document. For dated reference documents, only the dated version applies to this document. For undated references, the latest version (including all amendments) applies to this document.

GB 19489 Laboratory Biosafety General Requirements

3 Abbreviations

The following abbreviations apply to this document.

ASF. African Swine Fever (AfricanSwineFever)

ASFV. African swine fever virus (AfricanSwineFeverVirus)

DEPC. Diethyl Pyrocarbonate (DiethyPyrocarbonate)

EDTA. Ethylenediaminetetraacetic Acid (Ethylenediaminetetraacetic Acid)

ELISA. Enzyme Linked Immunosorbent Assay (EnzymeLinkedImmunosorbentAssay)

FAM. 6-Carboxy-Fluorescein (6-Carboxy-Fluorescein)

OD. Optical Density (OpticalDensity)

PBS. Phosphate Buffered Saline (PhosphateBufferedSaline)

PCR. Polymerase Chain Reaction (PolymeraseChain Reaction)

RAA. Recombinase-mediated isothermal nucleic acid amplification technology (RecombinaseAidedAmplification)

SPF. No specific pathogen (SpecificPathogenFree)

TMB. Tetramethyl-Benzidine (3,3',5,5'-Tetramethyl-Benzidine)

4 Biosecurity measures

When performing ASF laboratory diagnosis, such as sample processing, nucleic acid extraction, etc., should be implemented in accordance with GB 19489.

5 Clinical diagnosis