GB/T 17999.8-2008SPF chicken - Microbiological surveillance - Part 8: Examination of salmonella pullorum for SPF chicken (English PDF)
SPF鸡 微生物学监测 第8部分：SPF鸡 鸡白痢沙门氏菌检验
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Issued by
SAMR; SAC
Level / Type
National · Recommended
Issue date
December 31, 2008
Implementation date
May 1, 2009
Scope
GB/T 17999.8-2008 is the English-translated version of SPF鸡 微生物学监测 第8部分：SPF鸡 鸡白痢沙门氏菌检验.
GB/T 17999.8-2008 is the Chinese standard "SPF chicken - Microbiological surveillance - Part 8: Examination of salmonella pullorum for SPF chicken". Its scope clause reads: This section GB/T 17999 specifies the technical requirements for Salmonella testing.
This section applies to SPF chickens were separated and identified Salmonella pullorum. Serum plate agglutination test carried out to detect Salmonella antibody, the result is difficult to judge, it can be part of this re-examination.
Principle 2 Take sample to be assayed (intestinal contents, related to organ homogenates, live poultry cloacal swabs) was inoculated into enrichment medium enrichment is carried out, then the culture Yang was transferred to the selection, identification of the culture medium, the selection of suspicious colonies were inoculated on nutrient agar, the culture was subjected to biochemical tests take And serology tests to identify Salmonella. Its clauses include reagents and equipment; steps.
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Document preview — GB/T 17999.8-2008
National Standard of the People's Republic of China
- ICS
- 11.220
- Classification
- B 41
- Replacing
- GB/T 17999.7-1999
Issued by: State Administration for Market Regulation; Standardization Administration of the PRC
Contents
- 1 Scope
- 3 Reagents and Equipment
- 3.1 Materials
- 3.2 Equipment
- 4 steps
- 4.1 Sampling
- 4.2 Isolation
- 4.3 Pathogen Identification
1 Scope
This section GB/T 17999 specifies the technical requirements for Salmonella testing.
This section applies to SPF chickens were separated and identified Salmonella pullorum.
Serum plate agglutination test carried out to detect Salmonella antibody, the result is difficult to judge, it can be part of this re-examination.
Principle 2
Take sample to be assayed (intestinal contents, related to organ homogenates, live poultry cloacal swabs) was inoculated into enrichment medium enrichment is carried out, then the culture
Yang was transferred to the selection, identification of the culture medium, the selection of suspicious colonies were inoculated on nutrient agar, the culture was subjected to biochemical tests take
And serology tests to identify Salmonella.
3 Reagents and Equipment
3.1 Materials
3.1.1 Medium
Nutrient broth, DHL agar, the SS agar, bismuth sulfite agar (the BS), triple sugar iron medium (the TSI), nutrient agar, semisolid
Agar.
3.1.2 biochemical reagents
Sugar fermentation medium, peptone water, nitrate media, reagents oxidase, amino acid decarboxylase test medium, urea medium (Results
Determination see Appendix A).
3.1.3 Salmonella diagnostic sera
A-F O polyvalent serum, O9 serum factor, O12 serum factor, H-a serum factor, H-d serum factor, H-g. m factor and serum
H-g. p factor serum.
3.2 Equipment
37 °C incubator.
4 steps
4.1 Sampling
Aseptically ovary, liver, spleen and small intestine and cecum, live poultry cloacal swabs.
4.2 Isolation
The collected samples were placed in sterile homogenizer mortar or milled into a homogenized, add a small amount of nutrient broth dilution. Take culture or live poultry cloacal
Cavity swabs were on the SS or BS and DHL agar plate streaked, set (36 ± 1) °C cultured 24h ~ 48h. In DHL If there is a medium brown transparent small colonies; or appear colorless translucent small circular colonies on agar plates SS; or BS agar
Board a black or dark green small colonies, was suspicious colonies. No If, after 24h ~ 48h culture of suspicious colonies, then take enrichment
Repeat streaking cultures incubated for 1 times.
4.3 Pathogen Identification
4.3.1 microscopy
Suspected colonies, smears for Gram stain, specific operations, see Appendix A. Examination shows Gram-negative bacilli, the size of […]
......
This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 15 pages — is available in the English PDF.
Similar standards
Editions of GB/T 17999.8
| Edition | Title | Revision | Status |
|---|---|---|---|
| GB/T 17999.8-2008 | SPF chicken - Microbiological surveillance - Part 8: Examination of salmonella pullorum for SPF chicken | current edition | Current |
| GB/T 17999.7-1999 | SPF chicken - Microbiological surveillance - Part 8: Examination of salmonella pullorum for SPF chicken | previous edition | Superseded |
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Related Standards
GB/T 17999.1-2008 — SPF chicken - Microbiological surveillance - Part 1: General rules for the microbiological surveillance for SPF chicken
GB/T 17999.10-2008 — SPF chicken - Microbiological surveillance - Part 10: Indirect immunofluorescent assay for SPF chicken
GB/T 17999.2-2008 — SPF chicken - Microbiological surveillance - Part 2: Hemagglutination inhibition test for SPF chicken
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