GB/T 17999.7-2008SPF chicken - Microbiological surveillance - Part 7: Embryo susceptibility test for SPF chicken (English PDF)
SPF鸡 微生物学监测 第7部分：SPF鸡 胚敏感试验
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Issued by
SAMR; SAC
Level / Type
National · Recommended
Issue date
December 31, 2008
Implementation date
May 1, 2009
Scope
GB/T 17999.7-2008 is the English-translated version of SPF鸡 微生物学监测 第7部分：SPF鸡 胚敏感试验.
GB/T 17999.7-2008 is the Chinese standard "SPF chicken - Microbiological surveillance - Part 7: Embryo susceptibility test for SPF chicken". Its scope clause reads: This section GB/T 17999 specifies the technical requirements for susceptibility testing of embryos.
This section applies to SPF chickens were detected avian encephalomyelitis virus antibodies. Its clauses include reagents and equipment; procedure.
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Document preview — GB/T 17999.7-2008
National Standard of the People's Republic of China
- ICS
- 11.220
- Classification
- B 41
- Replacing
- GB/T 17999.6-1999
Issued by: State Administration for Market Regulation; Standardization Administration of the PRC
Contents
- 1 Scope
- 2 Normative references
- 4 Reagents and Equipment
- 5 Procedure
1 Scope
This section GB/T 17999 specifies the technical requirements for susceptibility testing of embryos.
This section applies to SPF chickens were detected avian encephalomyelitis virus antibodies.
2 Normative references
The following documents contain provisions which, through reference GB/T 17999 in this section constitute provisions of this section. For dated reference documents
Member, all subsequent amendments (not including errata content) or revisions do not apply to this section, however, encouraged to reach under this section
Parties to research agreement to use the latest versions of these documents. For undated reference documents, the latest versions apply to this section.
GB/T 17999.1-2008 SPF chickens microbiological monitoring - Part 1. SPF chicken microbiological monitoring General
Principle 3
VanRoekel (CVCCAV35) strain is a strain of avian embryos adapt strains highly neurotropic. None of the anti
Chicken flocks body pathogenic, causing muscular dystrophy, motor decreases, toes curling characteristic lesions. Immune or infected flocks
After the chick embryo yolk sac inoculation of virus resistance.
4 Reagents and Equipment
4.1 Reagents
4.1.1
AE-VanRoekel virus (EID50 determination see Appendix A).
4.1.2
SPF eggs.
4.1.3
is (see sampling ratio GB/T 17999.1-2008) seized eggs.
4.2 Equipment
4.2.1
1mL syringe.
4.2.2
the 7th needle.
4.2.3
incubator.
4.2.4
clean bench.
4.2.5
Small incubator.
5 Procedure
5.1
Take the subject eggs, standard SPF eggs hatch to 6 days of age.
5.2
Take 6-day-old chick embryos were seized, each embryo by yolk sac inoculation (inoculation method see Appendix A) 0.2mL containing 100EID50 the AE-Van
Roekel virus was inoculated in complete sealing wax, and set 6-day-old SPF chicken embryos inoculated with the virus and not the virus inoculation were used as a standard positive,
Negative control.
5.3
inoculated chicken embryos, placed in 37 °C ~ 37.5 °C incubator hatching, once a day, according to the embryo, remove dead embryo, the first 12d after inoculation
Remove all the chicken to be seized.
......
This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 15 pages — is available in the English PDF.
Similar standards
Editions of GB/T 17999.7
| Edition | Title | Revision | Status |
|---|---|---|---|
| GB/T 17999.7-2008 | SPF chicken - Microbiological surveillance - Part 7: Embryo susceptibility test for SPF chicken | current edition | Current |
| GB/T 17999.6-1999 | SPF chicken - Microbiological surveillance - Part 7: Embryo susceptibility test for SPF chicken | previous edition | Superseded |
This page sells the current edition, GB/T 17999.7-2008. Earlier editions are listed for reference only.
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Related Standards
GB/T 17999.1-2008 — SPF chicken - Microbiological surveillance - Part 1: General rules for the microbiological surveillance for SPF chicken
GB/T 17999.10-2008 — SPF chicken - Microbiological surveillance - Part 10: Indirect immunofluorescent assay for SPF chicken
GB/T 17999.2-2008 — SPF chicken - Microbiological surveillance - Part 2: Hemagglutination inhibition test for SPF chicken
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