GB/T 17999.10-2008SPF chicken - Microbiological surveillance - Part 10: Indirect immunofluorescent assay for SPF chicken (English PDF)

Foreword

GB/T 17999 "SPF chickens microbiological monitoring" is divided into 10 parts.

--- The first part 1. SPF chicken microbiological monitoring General; Part

--- Article 2. SPF chicken hemagglutination inhibition test; Part

--- Section 3. SPF chicken serum neutralization test; Part

--- Chapter 4. SPF chicken serum plate agglutination test; Part

--- Article 5. SPF chicken agar diffusion test;

--- Part 6. SPF chickens ELISA; Part

--- Article 7. SPF chicken embryo susceptibility testing; Part

--- Article 8. SPF chicken Salmonella testing;

--- Part 9. SPF chickens tube agglutination test; Part

--- Article 10. SPF chickens indirect immunofluorescence assay. This section GB/T 17999 Part 10. This Part replaces GB/T 17999.9-1999 "SPF chickens indirect immunofluorescence assay." This section compared with the GB/T 17999.9-1999 The main changes are as follows:

--- Increasing the normative Appendix A "Preparation of Reagents" and informative Appendix B "cell counting method";

--- A detailed definition of the experimental conditions and the establishment of sample to be tested criteria. Appendix A of this section is normative appendix, Appendix B is an informative annex. This section proposed by the People's Republic of China Ministry of Agriculture. This part of the National Animal Epidemic Prevention Standardization Technical Committee (SAC/TC181) centralized. This section is drafted. Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, China Animal Health and Epidemiology Center, Jinan Spa law Adams Poultry Limited. The main drafters of this section. song even East Liujia Sen, Han Lingxia, satellite Shao, Zhu fruit, single Zhong Fang, Jiang Qian, Secretary Chand, Yu Haibo, Mengqing Wen. This part of the standard replaces the previous editions are.

--- GB/T 17999.9-1999. SPF chickens microbiological monitoring Part 10. SPF chickens indirect immunofluorescence assay

1 Scope

GB/T 17999.10-2008 is the Chinese national standard on spf chicken - microbiological surveillance - part 10: indirect immunofluorescent assay for spf chicken, in the field of health care technology. The /T suffix marks it as a recommended standard: it is not compulsory by itself, but it becomes binding as soon as a contract, a tender or a customer specification calls it up - which in practice is how most foreign buyers meet it. It was issued on 31 December 2008 by the General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China; Standardization Administration of China, and has been in force since 1 May 2009. Classification: ICS 11.220, CCS B41. This page is published from the official record of the standard held by the Chinese standards administration: the identification, the dates, the classification and the issuing body are taken from there. The clause text, the tables and the numeric limits are in the document itself, which is delivered complete in English translation.

This section GB/T 17999 specifies the indirect immunofluorescence test technical requirements. This section applies to SPF chickens chicken infectious anemia virus (ChickenInfectiousAnaemiaVirus, CIAV) antibody Testing. Principle

2 Indirect immunofluorescence test for virus-infected cells as antigen and serum to be tested in the specific antibody binding, and then labeled with a fluorescent dye Anti-antibody binding, the formation of the antigen - antibody - anti-antibody complexes. Fluorescent dye under the action of ultraviolet light or violet light, a visible fluorescence excitation Light, and therefore appear to explain the presence of fluorescent markers, but also reflects the presence of specific antibodies. Often using a known antigen and a fluorescent dye Labeled anti-antibody to detect the corresponding antibodies.