GB/T 18089-2008Isolation, identification and serum neutralization antibody test in bluetongue virus (English PDF)

Foreword

This standard replaces GB/T 18089-2000 "Bluetongue micro serum neutralization test and the isolation and identification of the virus." This standard and GB/T 18089-2000 compared to the main changes are as follows:

--- Standard name has been modified;

--- Remove the bluetongue virus identified in a virus neutralization test portion;

--- Increase in the identification of viral identification RT-PCR method. Appendix A of this standard is a normative appendix. The standard proposed by the People's Republic of China Ministry of Agriculture. This standard by the National Standardization Technical Committee on Animal Epidemic Prevention. This standard was drafted. People's Republic of China Yunnan Exit Inspection and Quarantine, Shenzhen People's Republic of China Entry-Exit Inspection and Quarantine Bureau. The main drafters of this standard. Zhou Xiaoli, Yang Jianming, Ai Jun, flower Qun Yi, Yang flower, Zhangqi Yan, Yan tree hair, Dong Jun, Ye Lingling Xu Vega. This standard replaces the standards previously issued as follows:

--- GB/T 18089-2000. Bluetongue virus isolation, identification and serum and Antibody detection technology

1 Scope

GB/T 18089-2008 is the Chinese national standard on isolation, identification and serum neutralization antibody test in bluetongue virus, in the field of health care technology. The /T suffix marks it as a recommended standard: it is not compulsory by itself, but it becomes binding as soon as a contract, a tender or a customer specification calls it up - which in practice is how most foreign buyers meet it. It was issued on 31 December 2008 by the General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China; Standardization Administration of China, and has been in force since 1 May 2009. Classification: ICS 11.220, CCS B41. This page is published from the official record of the standard held by the Chinese standards administration: the identification, the dates, the classification and the issuing body are taken from there. The clause text, the tables and the numeric limits are in the document itself, which is delivered complete in English translation.

This standard specifies the bluetongue virus isolation, identification and serum neutralizing antibody detection techniques. This standard applies to the bluetongue virus isolation, identification and animal infected with bluetongue virus serum neutralizing antibody test.

2 Symbols and Abbreviations

The following symbols and abbreviations apply to this standard. BHK21

--- baby hamster kidney cell line; BLP

--- buffered lactose peptone broth; BT

--- bluetongue; bp

--- base pairs; CPE

--- induced cytopathic effect; C6/36

--- mosquito cell lines;

--- Of DEPC diethylpyrocarbonate; SPF

--- specific pathogen; TCID50

--- median tissue culture infective dose; Vero

--- African green monkey kidney cell line.

3 Bluetongue virus isolation

3.1 Materials 10 days to 11-day-old SPF chicken embryos.

3.2 Equipment and Apparatus Chick opening, Egg or Egg lamp box, a mortar or tissue masher, inverted microscope.