GB/T 17999.3-2008SPF chicken - Microbiological surveillance - Part 3: Serum neutralization test for SPF chicken (English PDF)
SPF鸡 微生物学监测 第3部分：SPF鸡 血清中和试验
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Issued by
SAMR; SAC
Level / Type
National · Recommended
Issue date
December 31, 2008
Implementation date
May 1, 2009
Scope
GB/T 17999.3-2008 is the English-translated version of SPF鸡 微生物学监测 第3部分：SPF鸡 血清中和试验.
GB/T 17999.3-2008 is the Chinese standard "SPF chicken - Microbiological surveillance - Part 3: Serum neutralization test for SPF chicken". Its scope clause reads: This section GB/T 17999 specifies the technical requirements and test serum.
This section applies to the following SPF chickens virus neutralizing antibody detection. AEV (AvianEncephalomyelitis Virus), infectious bronchitis virus (InfectiousBronchitisVirus), infectious laryngotracheitis virus (InfectiousLaryngo- tracheitisVirus), infectious bursal disease virus (InfectiousBursalDiseaseVirus).
Principle 2 Virus neutralization test means an antibody binding with the corresponding biological activity, the loss of their biological activity and response, and Anti Should not only highly specific, and has a strict relation between the amount of, and therefore can be used in tests to identify the virus, it can also be used to quantify the relative antibodies Testing. Its clauses include reagents and equipment.
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Document preview — GB/T 17999.3-2008
National Standard of the People's Republic of China
- ICS
- 11.220
- Classification
- B 41
- Replacing
- GB/T 17999.2-1999
Issued by: State Administration for Market Regulation; Standardization Administration of the PRC
Contents
- 1 Scope
- 3 Reagents and Equipment
- 3.1 Reagents
- 3.2 Equipment
- 4.1 Test preparation
1 Scope
This section GB/T 17999 specifies the technical requirements and test serum.
This section applies to the following SPF chickens virus neutralizing antibody detection. AEV (AvianEncephalomyelitis
Virus), infectious bronchitis virus (InfectiousBronchitisVirus), infectious laryngotracheitis virus (InfectiousLaryngo- tracheitisVirus), infectious bursal disease virus (InfectiousBursalDiseaseVirus).
Principle 2
Virus neutralization test means an antibody binding with the corresponding biological activity, the loss of their biological activity and response. And Anti
Should not only highly specific, and has a strict relation between the amount of. And therefore can be used in tests to identify the virus, it can also be used to quantify the relative antibodies
Testing.
3 Reagents and Equipment
3.1 Reagents
3.1.1 Virus
VanRoekel avian strain (CVCCAV35), infectious bronchitis virus H52 (CVCC AV1513), infectious bursal disease virus strain A80 (CVCVAV2321), infectious laryngotracheitis virus A96 (CVCCAV200).
3.1.2 virus dilutions
tryptone phosphate broth (see Appendix A).
3.1.3
penicillin/streptomycin (double-antibody) solution (see Appendix A).
3.1.5
SPF embryo.
3.2 Equipment
3.2.1
Egg drilling (hole punch).
3.2.4
mixer.
3.2.5
Eggtester.
Procedure 4
4.1 Test preparation
4.1.1 serum sample
Subject serum and positive control sera 56 °C, 30min ~ 45min inactivated complement, stored at -20 °C condition standby.
4.1.2 SPF Peidan ready
According embryo embryo viability check, select vessels clear, the embryo is healthy embryo swing law, each test item is required Peidan 24.
4.1.3 diluted virus
4.1.3.1
take sterile tubes 4 ~ 9, was added to each tube dilution 4mL, penicillin/streptomycin solution 0.5mL.
4.1.3.2
The first one added to the virus solution 0.5mL, for successive 10-fold dilution. Dilution of various viruses in Table 1.
......
This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 15 pages — is available in the English PDF.
Similar standards
Editions of GB/T 17999.3
| Edition | Title | Revision | Status |
|---|---|---|---|
| GB/T 17999.3-2008 | SPF chicken - Microbiological surveillance - Part 3: Serum neutralization test for SPF chicken | current edition | Current |
| GB/T 17999.2-1999 | SPF chicken - Microbiological surveillance - Part 3: Serum neutralization test for SPF chicken | previous edition | Superseded |
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Related Standards
GB/T 17999.1-2008 — SPF chicken - Microbiological surveillance - Part 1: General rules for the microbiological surveillance for SPF chicken
GB/T 17999.10-2008 — SPF chicken - Microbiological surveillance - Part 10: Indirect immunofluorescent assay for SPF chicken
GB/T 17999.2-2008 — SPF chicken - Microbiological surveillance - Part 2: Hemagglutination inhibition test for SPF chicken
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GB/T 17999.3-2008
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