GB/T 17999.4-2008SPF chicken - Microbiological surveillance - Part 4: Serum plate agglutination test for SPF chicken (English PDF)
SPF鸡 微生物学监测 第4部分：SPF鸡 血清平板凝集试验
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Issued by
SAMR; SAC
Level / Type
National · Recommended
Issue date
December 31, 2008
Implementation date
May 1, 2009
Scope
GB/T 17999.4-2008 is the English-translated version of SPF鸡 微生物学监测 第4部分：SPF鸡 血清平板凝集试验.
GB/T 17999.4-2008 is the Chinese standard "SPF chicken - Microbiological surveillance - Part 4: Serum plate agglutination test for SPF chicken". Its scope clause reads: This section GB/T 17999 specifies the technical requirements for chicken serum plate agglutination test.
Principle 2 Serum plate agglutination test is a bacterial antigen and the corresponding antibody binding, in the right amount of electrolyte participation, after a period of time appear Visible agglutination, often using a known standard liquid bacterial antigens corresponding detection antibody agglutination. Its clauses include reagents and materials.
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Document preview — GB/T 17999.4-2008
National Standard of the People's Republic of China
- ICS
- 11.220
- Classification
- B 41
- Replacing
- GB/T 17999.3-1999
Issued by: State Administration for Market Regulation; Standardization Administration of the PRC
Contents
- 1 Scope
- 3 Reagents and materials
- 3.1 Reagents
- 3.2 Material
1 Scope
This section GB/T 17999 specifies the technical requirements for chicken serum plate agglutination test.
Principle 2
Serum plate agglutination test is a bacterial antigen and the corresponding antibody binding, in the right amount of electrolyte participation, after a period of time appear
Visible agglutination, often using a known standard liquid bacterial antigens corresponding detection antibody agglutination.
3 Reagents and materials
3.1 Reagents
3.1.1
agglutination antigen.
3.1.2
negative sera, sera.
3.1.3
was tested serum.
3.2 Material
Glass pipette (TD10µL ~ 200µL) and tips.
Procedure 4
10min ~ 20min ago 4.1 Remove from the refrigerator antigen negative standard serum, the positive standard serum and serum were seized, to reach
Room temperature.
4.2
Mix well to draw a drop of diagnostic antigen (0.025mL ~ 0.05mL) with a pipette, vertical drops on the glass, then quickly
Next to dropping the same amount of antigen in the serum of the subject drop (0.025mL ~ 0.05mL).
4.3
with a toothpick serum mixed with the antigen coated sheet into 1cm ~ 2cm in diameter, constantly shaking the glass, 2min Vipassana
Police found.
4.4
The test shall be carried out at 20 °C ~ 25 °C conditions.
4.5
each batch of test serum should be set positive and negative serum and saline control.
5 results found
50% occurred within 2min (++) above as positive agglutination. No agglutination within 2min (-) were negative, between two of the above
Room as suspicious, reviewed the test, such as still suspicious, judged as positive. Agglutination interpretation criteria in Table 1.
......
This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 15 pages — is available in the English PDF.
Similar standards
Editions of GB/T 17999.4
| Edition | Title | Revision | Status |
|---|---|---|---|
| GB/T 17999.4-2008 | SPF chicken - Microbiological surveillance - Part 4: Serum plate agglutination test for SPF chicken | current edition | Current |
| GB/T 17999.3-1999 | SPF chicken - Microbiological surveillance - Part 4: Serum plate agglutination test for SPF chicken | previous edition | Superseded |
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Related Standards
GB/T 17999.1-2008 — SPF chicken - Microbiological surveillance - Part 1: General rules for the microbiological surveillance for SPF chicken
GB/T 17999.10-2008 — SPF chicken - Microbiological surveillance - Part 10: Indirect immunofluorescent assay for SPF chicken
GB/T 17999.2-2008 — SPF chicken - Microbiological surveillance - Part 2: Hemagglutination inhibition test for SPF chicken
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GB/T 17999.4-2008
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