GB 27953-2020General requirements on disinfectant for infectious focus (English PDF)

7.1 Selection of commonly-used disinfectant

7.1.1 Determine the commonly used disinfectants according to the types and resistance of contaminated pathogens

7.1.1.1 Prion contaminants: select chlorine disinfectant or sodium hydroxide, combined with pressure steam for sterilization method.

7.1.1.2 Spore contaminants (such as Bacillus anthracis, Clostridium tetani, etc.): Select disinfectants containing chlorine, peroxides, bromine and formaldehyde, etc.

7.1.1.3 Mycobacteria (such as Mycobacterium tuberculosis, Mycobacterium leprae), hydrophilic viruses (such as poliovirus, norovirus, adenovirus, rotavirus, hepatitis A virus, hepatitis E virus and pathogens that cause hand, foot and mouth disease), mycoplasma, chlamydia, rickettsiae and other pathogen contaminants: Select disinfectants containing chlorine, bromine, peroxides, aldehydes and iodine, etc.

7.1.1.4 Bacterial propagules (such as Vibrio cholerae, Shigella shigae, Corynebacterium diphtheriae, Salmonella typhi and Paratyphoid, Brucella, Neisseria gonorrhoeae, etc.), Nonlipid-coated viruses (such as influenza virus, measles virus, Hantavirus, etc.) and the contaminants of pathogens such as spirochetes: select disinfectants containing chlorine, bromine, peroxides, aldehydes, iodine, alcohols, guanidines, quaternary ammonium salts, etc.

7.1.1.5 For contaminants of pathogens (such as hepatitis B virus, hepatitis C virus, hepatitis D virus, human immunodeficiency virus, etc.) that are susceptible to organic matter and cause serious diseases, high-level disinfectants shall be used, such as disinfectants containing chlorine, bromine and peroxide, etc.

7.1.1.6 For contaminants of pathogens of special infectious diseases (such as SARS- Coronavirus, MERS-Coronavirus, Ebola virus, highly pathogenic avian influenza virus, H7N9 avian influenza virus, Yersinia pestis and rabies virus), it shall be carried out according to the relevant guideline formulated by the country.

7.1.1.7 For contaminants of unidentified pathogens, determine the applicable disinfectant according to 7.1.1.2.

7.1.2 Commonly-used disinfectants determined according to the disinfection objects contaminated by pathogens

7.1.2.1 Commonly-used surface disinfectants: chlorine-containing, bromine-containing and peroxide disinfectants, etc.

7.1.2.2 Commonly-used air disinfectants: peroxide disinfectants (such as peracetic acid, chlorine dioxide, hydrogen peroxide, ozone, etc.).

7.1.2.3 Commonly-used sewage disinfectants: chlorine-containing, bromine- containing and peroxide disinfectants, etc.

7.1.2.4 Commonly-used disinfectants for tableware: chlorine-containing, bromine- containing and peroxide disinfectants, etc.

7.1.2.5 Commonly-used disinfectants for excreta, secretions and corpse: chlorine- containing and peroxide disinfectants.

7.2 Use methods of commonly-used disinfectants

7.2.1 Disinfection methods used by prion disinfectants Table 1 -- Applicable Objects, Dosage and Use Method of Chlorine-Containing Disinfectants Disinfection objects Spore contaminants Mycobacterium and hydrophilic virus contaminants Contaminants of bacterial propagules and nonlipid-coated virus Use method Dosage Use method Dosage Use method Dosage Environment surface Rubbing, immersing, spraying 10000mg/L~15000mg/L available chlorine; acting for 2h; dosage of 100mL/m2 ~ 300mL/m

2 Rubbing, immersing, spraying 1000mg/L~2000mg/L available chlorine; acting for 1h; dosage of 100mL/m2~300mL/m

2 Rubbing, immersing, spraying 500mg/L~1000mg/L available chlorine; acting for 1h; dosage of 100mL/m2~300mL/m

2 Tableware Immersing 5000mg/L~10000mg/L available chlorine acts for 1h Immersing 1000mg/L~2000mg/L available chlorine acts for 0.5h immersing 250mg/L~500mg/L available chlorine acts for 0.5h Excreta, secretions Immersing Thin excreta and vomit: 1L of thin excreta and vomit is added with 50g of bleaching powder or 2L of 20000mg/L available chlorine disinfectant solution; stir evenly, and stand for 6h Formed feces: add 2 portions of 50000mg/L available chlorine disinfectant solution into 1 portion of feces; mix evenly, act for 6h Urine: every 1L of urine is added with 5g of bleaching power or 1.5g of calcium hypochlorite or immersing Thin excreta and vomit: 1L of thin excreta and vomit is added with 50g of bleaching powder or 2L of chlorine-containing disinfectant solution with 20000mg/L available chlorine; stir evenly, and stand for 2h Formed feces: add 2 portions of 50000mg/L available chlorine disinfectant solution into 1 portion of feces; mix evenly, act for 2h Urine: every 1L of urine is added with 5g of bleaching power or 1.5g of calcium hypochlorite or Immersing Thin excreta and vomit: 2L of thin excreta and vomit is added with 50g of bleaching powder or 2L of chlorine-containing disinfectant solution with 20000mg/L available chlorine; stir evenly, and stand for 2h Formed feces: add 2 portions of 50000mg/L available chlorine disinfectant solution into 1 portion of feces; mix evenly, act for 2h Urine: every 2L of urine is added with 5g of bleaching power or 1.5g of calcium hypochlorite or 100mL Table 2 -- Applicable Objects, Dosage and Use Method of Peroxide Disinfectants Disinfection objects Spore contaminants Mycobacterium and hydrophilic virus contaminants Contaminants of bacterial propagules and nonlipid-coated virus Use method Dosage Use method Dosage Use method Dosage Environment surface Rubbing, immersing, spraying 10000mg/L peracetic acid acts for 2h; and the dosage is 100mL/m2 ~ 300mL/m2. Or 60,000mg/L hydrogen peroxide acts for 2h. Or 2000mg/L chlorine dioxide acts for 2h Rubbing, immersing, spraying 5000mg/L peracetic acid acts for 1h; and the dosage is 100mL/m2 ~ 300mL/m2. Or 30,000mg/L hydrogen peroxide acts for 1h. Or 500mg/L~1000mg/L chlorine dioxide acts for 1h Rubbing, immersing, spraying Use 5000mg/L peracetic acid for the wall surface of mud floor; and spray 2000mg/L peracetic acid for the non-mud floor surface; the dosage is 100mL/m2 ~ 300mL/m2. Rub, immerse and disinfect for 1h. Or 30000mg/L hydrogen peroxide acts for 1h. Or 500mg/L chlorine dioxide acts for 0.5h Aerosol spray 20000mg/L peracetic acid acts for 1h ~ 2h, dosage 8mL/m

7.2.7 Use methods of formaldehyde fumigation

7.2.7.1 Overview The formaldehyde fumigation disinfection method may be used for disinfection of contaminated facilities and large equipment.

7.2.7.2 Heating fumigation 7.2.7.2.1 The fumigation and disinfection suitable for contaminants of bacterial spores, mycobacteria and hydrophilic virus: under the closed conditions at temperature >=18°C, relative humidity >=70%; the dosage is 25mL/m3 ~ 50 mL/m3 formaldehyde; conduct heating fumigation for 12h~24h. 7.2.7.2.2 The fumigation and disinfection suitable for contaminants of propagules and nonlipid-coated virus: under the conditions at temperature >=18°C and relative humidity >=70%; the dosage is

12.5 mL/m3~25 mL/m3 formaldehyde; conduct heating fumigation for 12h~24h.

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