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GB 19169-2003Pure culture of Auricularia auricula (English PDF)

黑木耳菌种

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Issued by

General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China

Level / Type

National · Mandatory

Issue date

June 4, 2003

Implementation date

December 1, 2003

Scope

GB 19169-2003 is the English-translated version of 黑木耳菌种.

GB 19169-2003 specifies the terms and definitions, quality requirements, test methods, inspection rules, and labelling, marking, packaging, transport and storage of the pure culture (spawn) of Auricularia auricula (black wood ear), and applies to its production, distribution and use.

It covers the three levels of the Chinese propagation system - stock culture in test tubes, pre-culture spawn and cultivation spawn - with sensory requirements for each (container, plug, fill, inoculum, mycelium appearance, exudates, contaminants, antagonism and sectors, ear buds, odour), microbiological requirements (hyphal morphology with clamp connections, no contaminants) and growth rates at 26 °C ± 2 °C: 10 to 15 days to cover the slant for stock culture, 40 to 45 days and 35 to 40 days to fill the container for pre-culture spawn and spawn.

Test methods cover sensory inspection, microscopic and cultural checks for moulds and bacteria, growth rate and a comparative cultivation test of the agronomic characters of the stock culture against the parent strain; sampling rates are 10 %, 5 % and 1 % by level, and retained samples are kept at 4 °C to 6 °C. Normative Annexes A and B give the culture media.

It is a first edition, issued on 4 June 2003 and implemented on 1 December 2003. Classification: ICS 65.020.20, CCS B39. The document runs to 10 pages, delivered complete in English translation.

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Document preview — GB 19169-2003

National Standard of the People's Republic of China

ICS: 65.020.20
Classification: B39

Issued by: General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China

Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Quality requirements
5 Sampling
6 Test methods
7 Inspection rules
8 Labelling, marking, packaging, transport and storage
Annex A (normative) Commonly used culture media for mother cultures and their formulas
Annex B (normative) Commonly used culture media for mother spawn and cultivated spawn and their formulas

3 Terms and definitions

4 Quality requirements

5 Sampling

6 Test methods

7 Inspection rules

8 Labelling, marking, packaging, transport and storage

Foreword

Annex A and Annex B of this standard are both normative annexes.

This standard was proposed by and is under the jurisdiction of the Ministry of Agriculture of the People's Republic of China.

Drafting organization of this standard: Huazhong Agricultural University.

Main drafters of this standard: Lü Zuozhou, Chen Liguo, Wang Zhuoren.

......
This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 10 pages — is available in the English PDF.

Referenced standards

Normative references

GB/T 4789.28

Cited by

LY/T 1207-2021Auriculaia auricula

Similar standards

GB 19170-2026|GB 19171-2026|GB 19172-2026|GB 4404.2-2026|GB/T 17315-2026|GB/T 17318-2026

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