GB 19171-2026Spawn of Agaricus bisporus (English PDF)

5.1 Basic Requirements

5.1.1 Species Requirements Mother culture, original culture, cultivated culture, and liquid culture should meet the requirements of variety characteristics.

5.1.2 Biological Requirements It should comply with the requirements in Table 2.

5.2 Mother Seed

5.2.1 Sensory Requirements It should comply with the requirements in Table 3.

5.2.2 Mycelial growth rate On potato dextrose agar (PDA) medium, cultured at 24°C±1°C, the culture medium surface was fully covered in 15-20 days.

5.3 Original and Cultivated Varieties

5.3.1 Sensory Requirements It should meet the requirements of Table 4, and the culture medium should be in close contact with the bottle (bag) wall without drying out.

5.3.2 Mycelial growth rate Incubate on grain culture medium, granular culture medium, or composted substrate culture medium at 24°C±1°C until the culture medium is fully confluent. More than 45 days.

5.4 Liquid bacterial cultures

5.4.1 Sensory Requirements It should comply with the requirements in Table 5.

5.4.2 Physicochemical Indicators It should comply with the requirements in Table 6.

6.3 Biological testing

6.3.1 Mycelial Examination The water-sealed slides of the culture were observed using an optical microscope with a magnification of at least 10×

40.Each sample should be observed for no less than [number missing]. 50 views.

6.3.2 Microbial Detection Prepare PDA or nutrient broth culture medium according to the formulas specified in Appendix A, A.1 and A.2, following the methods described in NY/T 1284. test.

6.3.3 Examination of insects and their eggs Test according to the method described in NY/T 1284.

6.4 Sensory inspection The amount of mother culture medium added, the appearance of the inoculum (excluding contaminating bacterial colonies), and the color and morphology of the liquid inoculum were visually inspected under natural light. Test; measure the length or thickness of the mother culture medium using appropriate measuring tools; inspect for contaminating microorganisms visually under natural light, using a magnifying glass or microscope if necessary. Observation and inspection; odor is tested by smell.

6.5 Test of mycelial growth rate

6.5.1 Mother Seed Prepare PDA medium according to the formula specified in A.1.After inoculation, incubate at 24°C±1°C and record the number of days required for the medium to reach full growth. Calculate the average of 3 repeated values.

6.5.2 Original species and cultivated species Prepare grain culture medium, granular culture medium, or composted substrate culture medium according to the formula specified in A.3, A.4, or A.5, and inoculate with the culture medium. The culture was then incubated at 24°C±1°C, and the number of days required for the culture medium to fully mature was recorded. The average value of the three replicates was calculated.

6.6 Physicochemical Indicators Testing of Liquid Microbial Strains

6.6.1 pH Prepare the liquid culture medium according to the formula specified in A.6.After inoculation and fermentation, measure a certain volume aseptically. The pH of the culture medium is measured using pH test paper or a pH meter.

6.6.2 Mycelial wet weight A random volume of culture medium sample was taken, centrifuged at 6000 r/min for 15 min, the supernatant was discarded, and the sample was weighed using a balance of appropriate precision. Hyphae quality. Calculate the mass-volume fraction of mycelia in the culture medium sample.

6.6.3 Mycelial volume ratio A random volume of culture medium sample was taken, allowed to stand for >=60 min, and the mycelial volume was measured using a measuring instrument of appropriate precision. The mycelial occupancy in the culture medium was then calculated. Volume ratio of nutrient solution sample (expressed as a percentage).

7.1 Batching

7.1.1 Mother Seed Products of the same variety, under the same culture conditions, and at the same inoculation time are considered as one inspection batch.

7.1.2 Original strain, cultivated strain, liquid inoculum Products of the same strain, from the same strain, using the same inoculation method, and at the same inoculation time are considered as one inspection batch.

7.2 Sampling Samples were randomly selected from each batch for testing. The sampling quantities for mother culture, primary culture, and cultivated culture were 10%, 5%, and 1% of the total amount of culture in that batch, respectively. However, each The batch sampling quantity should not be less than 10 vials (bottles, bags); for batches exceeding 100 vials (bottles, bags), two-stage sampling may be used. Each batch of liquid culture should be sampled. test.

7.3 Types of Inspection

7.3.1 Factory Inspection Each batch of products should undergo factory inspection. Factory inspection items include container requirements and sensory requirements; for liquid microbial cultures, physicochemical indicators should also be tested. Unless otherwise agreed by the purchasing party regarding the product, the agreement between the supplier and the purchasing party shall prevail.

7.3.2 Type Testing Type testing shall be conducted on all items specified in Chapter

4.Type testing shall be conducted in any of the following circumstances.

a) When production resumes after a shutdown of more than 6 months;

b) When the results of two consecutive sampling inspections differ significantly;

c) When changes in raw materials or alterations to the production process may affect product quality;

d) When there is a significant difference between the factory inspection and the previous type inspection;

e) When the national administrative regulatory department requests a type test;

f) During normal production, periodic inspections shall be conducted at least once a year.

7.4 Judgment Rules If all inspected items meet the requirements, the batch is deemed (qualified); if any basic requirement is not met, the batch is deemed (unqualified). If sensory evaluation or mycelial growth rate fails to meet standards, the producer is allowed to make corrections and apply for a re-inspection once; the re-inspection will still be conducted according to the original requirements, and the re-inspection results will be used. As the basis for the final judgment.