GB/T 8381.7-2009The determination of olaquindox in feed - High performance liquid chromatography (English PDF)
饲料中喹乙醇的测定 高效液相色谱法
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Issued by
SAMR; SAC
Level / Type
National · Recommended
Issue date
May 12, 2009
Implementation date
September 1, 2009
Scope
GB/T 8381.7-2009 is the English-translated version of 饲料中喹乙醇的测定 高效液相色谱法.
GB/T 8381.7-2009 is the Chinese standard "The determination of olaquindox in feed - High performance liquid chromatography". Its scope clause reads: This standard specifies the method for the determination of olaquindox in feed by high performance liquid chromatography (HPLC).
This standard is applicable to the determination of olaquindox in mixed feed, concentrated feed and additive premix feed with a minimum limit of 1 mg/kg, The detection limit was 0.1 mg/kg.
Its clauses include normative reference documents; principle; reagents and solutions; instruments.
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Document preview — GB/T 8381.7-2009
National Standard of the People's Republic of China
- ICS
- 65.120
- Classification
- B 46
- Replacing
- GB/T 8381.7-2005
Issued by: State Administration for Market Regulation; Standardization Administration of the PRC
Contents
- 1 Scope
- 2 normative reference documents
- 3 principle
- 4 reagents and solutions
- 5 instruments
1 Scope
This standard specifies the method for the determination of olaquindox in feed by high performance liquid chromatography (HPLC).
This standard is applicable to the determination of olaquindox in mixed feed, concentrated feed and additive premix feed with a minimum limit of 1 mg/kg,
The detection limit was 0.1 mg/kg.
2 normative reference documents
The terms of the following documents are hereby incorporated by reference into this standard. Whichever is the date of the reference file, which is followed by all (Excluding corrigenda) or revisions do not apply to this standard, however, encourage the parties to reach an agreement under this standard
Whether you can use the latest version of these files. For undated references, the latest edition applies to this standard.
GB/T 6682 Analytical laboratory water specifications and test methods (GB/T 6682-2008, ISO 3696. 1987, MOD)
3 principle
The olaquindox in the sample was extracted with methanol solution, purified by solid phase extraction, reversed phase liquid chromatography, UV detection,
Quantitative Analysis of External Standard Method.
4 reagents and solutions
Unless otherwise specified, only the reagents identified as analytical reagents and Grade III water in accordance with GB/T 6682 are used in the analysis.
WARNING - Olaquindox is sensitive to light, should be operated in a dark and use a brown container.
4.1
Methanol (chromatographic purity).
4.2 Extract
Methanol + water = 5 + 95.
4.3 High Performance Liquid Chromatography Mobile Phase
Methanol and Ultra Pure Water (Using Binary Gradient).
4.4 eluent 1
0.02 mol/L hydrochloric acid. Remove 1.67 mL of hydrochloric acid to volume to 1000 mL.
4.5 eluent 2
0.1mol/L hydrochloric acid. Remove 8.33 mL of hydrochloric acid to 10 mL.
4.6 eluent 3
methanol + water = 5 + 95.
4.7 Eluent
Methanol + water = 40 + 60.
4.8 olaquindox standard stock solution
accurately weighed olaquindox standard 0.05020g (content >= 99.6%), in 50mL brown volumetric flask,
Ultrasonic solution, cooled to room temperature, constant volume to the scale, shake, so that the solution concentration of 1mg/mL, stored in -18 °C refrigerator, you can use a
Month.
4.9 Olaquindox standard working solution
accurate measurement of the standard stock solution (4.8) in the volumetric flask, diluted with eluent (4.7), followed by preparation into the concentration
Was 0.1 µg/mL, 1.0 µg/mL, 5.0 µg/mL, 10.0 µg/mL, 20.0 µg/mL, 50.0 µg/mL, 100.0 µg/mL
Solution, with the current use.
5 instruments
5.1 Centrifuge
3500r/min.
5.2 shaker
speed up to 110r/min.
5.3 screw thread centrifuge tube
50mL.
......
This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 15 pages — is available in the English PDF.
Similar standards
Editions of GB/T 8381.7
| Edition | Title | Revision | Status |
|---|---|---|---|
| GB/T 8381.7-2009 | The determination of olaquindox in feed - High performance liquid chromatography | current edition | Current |
| GB/T 8381.7-2005 | The determination of olaquindox in feed - High performance liquid chromatography | previous edition | Superseded |
This page sells the current edition, GB/T 8381.7-2009. Earlier editions are listed for reference only.
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