GB/T 5523-2008Inspection of grains and oils - Determination of lipase activity of grain and oilseeds (English PDF)
粮油检验 粮食、油料的脂肪酶活动度的测定
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Issued by
SAMR; SAC
Level / Type
National · Recommended
Issue date
November 4, 2008
Implementation date
January 20, 2009
Scope
GB/T 5523-2008 is the English-translated version of 粮油检验 粮食、油料的脂肪酶活动度的测定.
GB/T 5523-2008 is the Chinese standard "Inspection of grains and oils - Determination of lipase activity of grain and oilseeds".
Its scope clause reads: This standard specifies the grain and oilseeds in lipase activity determination of terms and definitions, principles, reagents and materials, apparatus and appliances, sample Preparation, analysis step, and reproducibility of the results of calculation.
This standard applies to grain, oilseeds determination of Lipase activity. Its clauses include terms and definitions; reagents and materials.
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Document preview — GB/T 5523-2008
National Standard of the People's Republic of China
- ICS
- 67.040
- Classification
- X 04
- Replacing
- GB/T 5523-1985
Issued by: State Administration for Market Regulation; Standardization Administration of the PRC
Contents
- 1 Scope
- 2 Normative references
- 3 Terms and Definitions
- 5 Reagents and materials
1 Scope
This standard specifies the grain and oilseeds in lipase activity determination of terms and definitions, principles, reagents and materials, apparatus and appliances, sample
Preparation, analysis step, and reproducibility of the results of calculation.
This standard applies to grain, oilseeds determination of Lipase activity.
2 Normative references
The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent
Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research
Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard.
Preparation of GB/T 601 chemical reagent standard titration solution
GB 5491 grain and oilseeds testing for sampling, and sample method
GB/T 5497 grain and oilseeds - Determination of water test
GB/T 6682 analytical laboratory use specifications and test methods (GB/T 6682-2008, ISO 3696. 1987, MOD)
3 Terms and Definitions
The following terms and definitions apply to this standard.
3.1
The amount of a certain amount of lipase in the sample and the role of pure oil free fatty acids generated by the sample and 1g (dry basis) generated
Free fatty acids milligrams of potassium hydroxide consumed in Fig.
Principle 4
Under conditions of pH7.4, lipase extracted from the sample, and to act on an amount of pure oil, pure oil in the lower part of lipase
Decomposition divided into free fatty acids, and then titrated with potassium hydroxide solution.
5 Reagents and materials
The following reagents were analytical grade reagents.
5.1 Water
in line with GB/T 6682 laboratory water three requirements.
In 1000mL volumetric flask, with 95% (volume fraction) ethanol diluted volume to 1000mL, stored in polyethylene plastic bottles. Before use
Diluted formulation.
5.5
ethanol and anhydrous ether (4.1) mixture.
5.6
pH7.4 buffer.
A solution. Weigh-hydrogen phosphate, disodium 11.876g, dissolved in 1L of water.
......
This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 15 pages — is available in the English PDF.
Similar standards
Editions of GB/T 5523
| Edition | Title | Revision | Status |
|---|---|---|---|
| GB/T 5523-2008 | Inspection of grains and oils - Determination of lipase activity of grain and oilseeds | current edition | Current |
| GB/T 5523-1985 | Inspection of grains and oils - Determination of lipase activity of grain and oilseeds | previous edition | Superseded |
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