GB/T 5521-2008Inspection of grain and oils - Determination of alpha-amylase activity in cereal and cereal products - Colorimetric method (English PDF)
粮油检验 谷物及其制品中α-淀粉酶活性的测定 比色法
This is a limited preview
Buy now to download the full PDF (15 pages)
Need quotation for your management?
Get a formal quotation for GB/T 5521-2008 in 30 minutes.
Issued by
SAMR; SAC
Level / Type
National · Recommended
Issue date
November 4, 2008
Implementation date
January 1, 2009
Scope
GB/T 5521-2008 is the English-translated version of 粮油检验 谷物及其制品中α-淀粉酶活性的测定 比色法.
GB/T 5521-2008 is the Chinese standard "Inspection of grain and oils - Determination of alpha-amylase activity in cereal and cereal products - Colorimetric method".
Its scope clause reads: This standard specifies the colorimetry and cereal products alpha- amylase activity terms and definitions, principles, reagents and materials, equipment, operation Procedures, calculation results, precision and test reports.
This standard applies to the determination of cereals and cereal products alpha- amylase activity can also be used to determine derived from fungi and bacteria alpha- amylase powder Activity. Its clauses include terms and definitions; reagents and materials.
Found what you need?
Document preview — GB/T 5521-2008
National Standard of the People's Republic of China
- ICS
- 67.040
- Classification
- B 20
- Replacing
- GB/T 5521-1989
Issued by: State Administration for Market Regulation; Standardization Administration of the PRC
Contents
- 1 Scope
- 2 Normative references
- 3 Terms and Definitions
- 5 Reagents and materials
1 Scope
This standard specifies the colorimetry and cereal products alpha- amylase activity terms and definitions, principles, reagents and materials, equipment, operation
Procedures, calculation results, precision and test reports.
This standard applies to the determination of cereals and cereal products alpha- amylase activity can also be used to determine derived from fungi and bacteria alpha- amylase powder
Activity.
2 Normative references
The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent
Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to this part of the research agreement
Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard.
GB/T 6682 analytical laboratory use specifications and test methods (GB/T 6682-2008, ISO 3696. 1987, MOD)
3 Terms and Definitions
The following terms and definitions apply to this standard.
3.1
beta- amylase can be completely degraded starch product.
3.2
Samples extracted from 1g enzyme 1L solvent, under specified conditions, the degradation of beta- limit dextrin substrate per 1.024 × 10-5 units
Solution, alpha- amylase activity of the sample as a unit.
Principle 4
alpha- beta- amylase degradation limit dextrin substrate solution, the enzymatic reaction process, respectively, at different times aliquots of the reaction mixture was added iodine
Solution. With the reaction time, the reaction mixture of iodine and reduce color intensity to determine enzyme activity.
5 Reagents and materials
Unless otherwise indicated, the reagents used were of analytical grade. Test water line with GB/T 6682 in the three water requirements.
5.1
iodine.
5.2
potassium iodide.
5.3
calcium chloride.
5.4
anhydrous sodium acetate.
5.5
sulfuric acid.
5.6
glacial acetic acid.
5.7
soluble starch.
NOTE. to use Lintner starch or of comparable quality domestic soluble starch.
......
This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 15 pages — is available in the English PDF.
Similar standards
Editions of GB/T 5521
| Edition | Title | Revision | Status |
|---|---|---|---|
| GB/T 5521-2008 | Inspection of grain and oils - Determination of alpha-amylase activity in cereal and cereal products - Colorimetric method | current edition | Current |
| GB/T 5521-1989 | Inspection of grain and oils - Determination of alpha-amylase activity in cereal and cereal products - Colorimetric method | previous edition | Superseded |
This page sells the current edition, GB/T 5521-2008. Earlier editions are listed for reference only.
How to Download GB/T 5521-2008
- 1
Add to cart
Click "Download PDF" on this page and choose "Buy this standard". You can add more standards before checkout.
- 2
Checkout
Enter your email and billing details. Payment is processed securely by Stripe (cards or bank transfer). Prefer bank details? Request a quotation (min. order $300).
- 3
Instant delivery (0–9 sec)
Delivery is automatic: within seconds of payment you'll receive an email with a secure download link. The link stays valid for 72 hours.
- 4
Invoice included
A tax invoice is attached to the confirmation email. Need a custom invoice? Contact us.
Related Standards
GB/T 10003-2026 — Biaxially oriented polypropylene (BOPP) film
GB/T 10004-2026 — Plastic/Plastic laminated films and pouches for packaging
GB/T 10007-2026 — Rigid cellular plastics - Determination of shear properties
Secure payment via Stripe
Payments accepted
GB/T 5521-2008
$135.00