GB/T 5513-2019Inspection of grain and oils—Determination of reduced sugar and non-reduced sugar in grain (English PDF)
粮油检验 粮食中还原糖和非还原糖测定
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Issued by
SAMR; SAC
Level / Type
National · Recommended
Issue date
May 10, 2019
Implementation date
December 1, 2019
Scope
GB/T 5513-2019 is the English-translated version of 粮油检验 粮食中还原糖和非还原糖测定.
GB/T 5513-2019 is the Chinese standard "Inspection of grain and oils—Determination of reduced sugar and non-reduced sugar in grain". Its scope clause reads: This standard specifies the terms and definitions for the determination of reducing sugars and non-reducing sugars in food, as well as the methods of potassium ferricyanide and Ferien reagents.
The principles, reagents, instruments and utensils, procedure, calculation of results and repeatability of reducing sugars and non-reducing sugars were determined.
This standard is applicable to the determination of reducing sugar and non-reducing sugar content in commercial grain. The potassium ferricyanide method is mainly suitable for wheat flour reduction.
Determination of sugars and non-reducing sugars. Its clauses include terms and definitions; potassium ferricyanide method.
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Document preview — GB/T 5513-2019
National Standard of the People's Republic of China
- ICS
- 67.040
- Classification
- X 04
- Replacing
- GB/T 5513-2008
Issued by: State Administration for Market Regulation; Standardization Administration of the PRC
Contents
- 1 Scope
- 2 Normative references
- 3 Terms and definitions
- 4 potassium ferricyanide method
1 Scope
This standard specifies the terms and definitions for the determination of reducing sugars and non-reducing sugars in food, as well as the methods of potassium ferricyanide and Ferien reagents.
The principles, reagents, instruments and utensils, procedure, calculation of results and repeatability of reducing sugars and non-reducing sugars were determined.
This standard is applicable to the determination of reducing sugar and non-reducing sugar content in commercial grain. The potassium ferricyanide method is mainly suitable for wheat flour reduction.
Determination of sugars and non-reducing sugars.
2 Normative references
The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article.
Pieces. For undated references, the latest edition (including all amendments) applies to this document.
Preparation of GB/T 601 chemical reagent standard titration solution
GB/T 5490 General rules for grain and oil inspection
GB/T 6682 Analytical laboratory water specifications and test methods
3 Terms and definitions
The following terms and definitions apply to this document.
3.1 Reducing sugar reducedsugar
A sugar having a free hemiacetal hydroxyl group or a hemi-ketal hydroxyl group in its molecular structure.
3.2 Non-reducing sugar
A sugar which does not contain a free hemiacetal hydroxyl group or a hemi-ketal hydroxyl group in the molecular structure.
4 potassium ferricyanide method
4.1 Principle
The reducing sugar reduces potassium ferricyanide to potassium ferrocyanide in an alkaline solution and is itself oxidized to the corresponding sugar acid. Excess potassium ferricyanide
In the presence of acetic acid, iodine is precipitated by the action of potassium iodide, and the precipitated iodine is titrated with a sodium thiosulfate standard solution. By calculating redox sugar
The amount of potassium ferricyanide used is checked to show the percentage of reducing sugar in the sample.
4.2 Reagents
Unless otherwise stated, this standard uses only reagents that are confirmed to be analytically pure.
4.2.1 Water
Meet the requirements of Class III water in GB/T 6682.
4.2.3 Acetate buffer
Dissolve 3.0 mL of glacial acetic acid, 6.8 g of anhydrous sodium acetate and 4.5 mL of concentrated sulfuric acid, then dilute with water until
Mix 1000 mL and mix.
......
This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 15 pages — is available in the English PDF.
Similar standards
Editions of GB/T 5513
| Edition | Title | Revision | Status |
|---|---|---|---|
| GB/T 5513-2019 | Inspection of grain and oils—Determination of reduced sugar and non-reduced sugar in grain | current edition | Current |
| GB/T 5513-2008 | Inspection of grain and oils—Determination of reduced sugar and non-reduced sugar in grain | previous edition | Superseded |
This page sells the current edition, GB/T 5513-2019. Earlier editions are listed for reference only.
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